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Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed
INTRODUCTION: Two-line hybrid rice based on Photoperiod/thermo-sensitive genic male sterile (P/TGMS) lines has been developed and applied widely in agriculture due to the freedom in making hybrid combinations, less difficulty in breeding sterile lines, and simpler procedures for breeding and produci...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10435883/ https://www.ncbi.nlm.nih.gov/pubmed/37600184 http://dx.doi.org/10.3389/fpls.2023.1217893 |
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author | Zhang, Xiuli Wang, Qing Fan, Guojian Tang, Li Shao, Ye Mao, Bigang Lv, Qiming Zhao, Bingran |
author_facet | Zhang, Xiuli Wang, Qing Fan, Guojian Tang, Li Shao, Ye Mao, Bigang Lv, Qiming Zhao, Bingran |
author_sort | Zhang, Xiuli |
collection | PubMed |
description | INTRODUCTION: Two-line hybrid rice based on Photoperiod/thermo-sensitive genic male sterile (P/TGMS) lines has been developed and applied widely in agriculture due to the freedom in making hybrid combinations, less difficulty in breeding sterile lines, and simpler procedures for breeding and producing hybrid seed. However, there are certain risks associated with hybrid seed production; if the temperature during the P/TGMS fertility-sensitive period is lower than the critical temperature, seed production will fail due to self-pollination. In a previous study, we found that the issue of insufficient purity of two-line hybrid rice seed could be initially addressed by using the difference in tolerance to β-triketone herbicides (bTHs) between the female parent and the hybrid seeds. METHODS: In this study, we further investigated the types of applicable herbicides, application methods, application time, and the effects on physiological and biochemical indexes and yield in rice. RESULTS: The results showed that this method could be used for hybrid purification by soaking seeds and spraying plants with the bTH benzobicylon (BBC) at safe concentrations in the range of 37.5-112.5 mg/L, and the seeds could be soaked in BBC at a treatment rate of 75.0 mg/L for 36-55 h without significant negative effects. The safe concentration for spraying in the field is 50.0-400.0 mg/L BBC at the three-leaf stage. Unlike BBC, Mesotrione (MST) can only be sprayed to achieve hybrid purification at concentrations between 10.0 and 70.0 mg/L without affecting yield. The three methods of hybrid seed purification can reach 100% efficiency without compromising the nutritional growth and yield of hybrid rice. Moreover, transcriptome sequencing revealed that 299 up-regulated significant differentially expressed genes (DEGs) in the resistant material (Huazhan) poisoned by BBC, were mainly enriched in phenylalanine metabolism and phenylpropanoid biosynthesis pathway, it may eliminate the toxic effects of herbicides through this way. DISCUSSION: Our study establishes a foundation for the application of the bTH seed purification strategy and the three methods provide an effective mechanism for improving the purity of two-line hybrid rice seeds. |
format | Online Article Text |
id | pubmed-10435883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104358832023-08-19 Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed Zhang, Xiuli Wang, Qing Fan, Guojian Tang, Li Shao, Ye Mao, Bigang Lv, Qiming Zhao, Bingran Front Plant Sci Plant Science INTRODUCTION: Two-line hybrid rice based on Photoperiod/thermo-sensitive genic male sterile (P/TGMS) lines has been developed and applied widely in agriculture due to the freedom in making hybrid combinations, less difficulty in breeding sterile lines, and simpler procedures for breeding and producing hybrid seed. However, there are certain risks associated with hybrid seed production; if the temperature during the P/TGMS fertility-sensitive period is lower than the critical temperature, seed production will fail due to self-pollination. In a previous study, we found that the issue of insufficient purity of two-line hybrid rice seed could be initially addressed by using the difference in tolerance to β-triketone herbicides (bTHs) between the female parent and the hybrid seeds. METHODS: In this study, we further investigated the types of applicable herbicides, application methods, application time, and the effects on physiological and biochemical indexes and yield in rice. RESULTS: The results showed that this method could be used for hybrid purification by soaking seeds and spraying plants with the bTH benzobicylon (BBC) at safe concentrations in the range of 37.5-112.5 mg/L, and the seeds could be soaked in BBC at a treatment rate of 75.0 mg/L for 36-55 h without significant negative effects. The safe concentration for spraying in the field is 50.0-400.0 mg/L BBC at the three-leaf stage. Unlike BBC, Mesotrione (MST) can only be sprayed to achieve hybrid purification at concentrations between 10.0 and 70.0 mg/L without affecting yield. The three methods of hybrid seed purification can reach 100% efficiency without compromising the nutritional growth and yield of hybrid rice. Moreover, transcriptome sequencing revealed that 299 up-regulated significant differentially expressed genes (DEGs) in the resistant material (Huazhan) poisoned by BBC, were mainly enriched in phenylalanine metabolism and phenylpropanoid biosynthesis pathway, it may eliminate the toxic effects of herbicides through this way. DISCUSSION: Our study establishes a foundation for the application of the bTH seed purification strategy and the three methods provide an effective mechanism for improving the purity of two-line hybrid rice seeds. Frontiers Media S.A. 2023-08-03 /pmc/articles/PMC10435883/ /pubmed/37600184 http://dx.doi.org/10.3389/fpls.2023.1217893 Text en Copyright © 2023 Zhang, Wang, Fan, Tang, Shao, Mao, Lv and Zhao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Xiuli Wang, Qing Fan, Guojian Tang, Li Shao, Ye Mao, Bigang Lv, Qiming Zhao, Bingran Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
title | Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
title_full | Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
title_fullStr | Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
title_full_unstemmed | Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
title_short | Utilizing differences in bTH tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
title_sort | utilizing differences in bth tolerance between the parents of two-line hybrid rice to improve the purity of hybrid rice seed |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10435883/ https://www.ncbi.nlm.nih.gov/pubmed/37600184 http://dx.doi.org/10.3389/fpls.2023.1217893 |
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