Protocol for designing and bioprinting multi-layered constructs to reconstruct an endothelial-epithelial 3D model

3D bioprinting has opened new possibilities and elevated tissue engineering complexity. Here, we present a protocol to design a 3D model with two cell lineage layers (A549 and HUVEC) to recreate multi-cell constructs. We describe the steps for slicing the constructs, handling hydrogels, and detailin...

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Detalles Bibliográficos
Autores principales: Backes, Eduardo Henrique, Zamproni, Laura Nicoleti, Delgado-Garcia, Lina Maria, Pinto, Leonardo Alves, Lemes, Robertha Mariana Rodrigues, Bartolomeo, Cynthia Silva, Porcionatto, Marimélia Aparecida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436237/
https://www.ncbi.nlm.nih.gov/pubmed/37585294
http://dx.doi.org/10.1016/j.xpro.2023.102467

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436237/
https://www.ncbi.nlm.nih.gov/pubmed/37585294
http://dx.doi.org/10.1016/j.xpro.2023.102467

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