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Protocol for designing and bioprinting multi-layered constructs to reconstruct an endothelial-epithelial 3D model
3D bioprinting has opened new possibilities and elevated tissue engineering complexity. Here, we present a protocol to design a 3D model with two cell lineage layers (A549 and HUVEC) to recreate multi-cell constructs. We describe the steps for slicing the constructs, handling hydrogels, and detailin...
Autores principales: | Backes, Eduardo Henrique, Zamproni, Laura Nicoleti, Delgado-Garcia, Lina Maria, Pinto, Leonardo Alves, Lemes, Robertha Mariana Rodrigues, Bartolomeo, Cynthia Silva, Porcionatto, Marimélia Aparecida |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436237/ https://www.ncbi.nlm.nih.gov/pubmed/37585294 http://dx.doi.org/10.1016/j.xpro.2023.102467 |
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