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Tryptophan-PNA gc Conjugates Self-Assemble to Form Fibers
[Image: see text] Peptide nucleic acids and their conjugates to peptides can self-assemble and generate complex architectures. In this work, we explored the self-assembly of PNA dimers conjugated to the dipeptide WW. Our studies suggest that the indole ring of tryptophan promotes aggregation of the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436247/ https://www.ncbi.nlm.nih.gov/pubmed/37486977 http://dx.doi.org/10.1021/acs.bioconjchem.3c00200 |
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author | Mosseri, Andrea Sancho-Albero, María Mercurio, Flavia Anna Leone, Marilisa De Cola, Luisa Romanelli, Alessandra |
author_facet | Mosseri, Andrea Sancho-Albero, María Mercurio, Flavia Anna Leone, Marilisa De Cola, Luisa Romanelli, Alessandra |
author_sort | Mosseri, Andrea |
collection | PubMed |
description | [Image: see text] Peptide nucleic acids and their conjugates to peptides can self-assemble and generate complex architectures. In this work, we explored the self-assembly of PNA dimers conjugated to the dipeptide WW. Our studies suggest that the indole ring of tryptophan promotes aggregation of the conjugates. The onset of fluorescence is observed upon self-assembly. The structure of self-assembled WWgc is concentration-dependent, being spherical at low concentrations and fibrous at high concentrations. As suggested by molecular modeling studies, fibers are stabilized by stacking interactions between tryptophans and Watson-Crick hydrogen bonds between nucleobases. |
format | Online Article Text |
id | pubmed-10436247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104362472023-08-19 Tryptophan-PNA gc Conjugates Self-Assemble to Form Fibers Mosseri, Andrea Sancho-Albero, María Mercurio, Flavia Anna Leone, Marilisa De Cola, Luisa Romanelli, Alessandra Bioconjug Chem [Image: see text] Peptide nucleic acids and their conjugates to peptides can self-assemble and generate complex architectures. In this work, we explored the self-assembly of PNA dimers conjugated to the dipeptide WW. Our studies suggest that the indole ring of tryptophan promotes aggregation of the conjugates. The onset of fluorescence is observed upon self-assembly. The structure of self-assembled WWgc is concentration-dependent, being spherical at low concentrations and fibrous at high concentrations. As suggested by molecular modeling studies, fibers are stabilized by stacking interactions between tryptophans and Watson-Crick hydrogen bonds between nucleobases. American Chemical Society 2023-07-24 /pmc/articles/PMC10436247/ /pubmed/37486977 http://dx.doi.org/10.1021/acs.bioconjchem.3c00200 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Mosseri, Andrea Sancho-Albero, María Mercurio, Flavia Anna Leone, Marilisa De Cola, Luisa Romanelli, Alessandra Tryptophan-PNA gc Conjugates Self-Assemble to Form Fibers |
title | Tryptophan-PNA
gc Conjugates Self-Assemble to Form
Fibers |
title_full | Tryptophan-PNA
gc Conjugates Self-Assemble to Form
Fibers |
title_fullStr | Tryptophan-PNA
gc Conjugates Self-Assemble to Form
Fibers |
title_full_unstemmed | Tryptophan-PNA
gc Conjugates Self-Assemble to Form
Fibers |
title_short | Tryptophan-PNA
gc Conjugates Self-Assemble to Form
Fibers |
title_sort | tryptophan-pna
gc conjugates self-assemble to form
fibers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436247/ https://www.ncbi.nlm.nih.gov/pubmed/37486977 http://dx.doi.org/10.1021/acs.bioconjchem.3c00200 |
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