Cargando…
Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana
Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms c...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10437119/ https://www.ncbi.nlm.nih.gov/pubmed/37601384 http://dx.doi.org/10.3389/fmicb.2023.1211301 |
_version_ | 1785092439613112320 |
---|---|
author | Fan, Huacai He, Ping Xu, Shengtao Li, Shu Wang, Yongfen Zhang, Wenlong Li, Xundong Shang, Hui Zeng, Li Zheng, Si-Jun |
author_facet | Fan, Huacai He, Ping Xu, Shengtao Li, Shu Wang, Yongfen Zhang, Wenlong Li, Xundong Shang, Hui Zeng, Li Zheng, Si-Jun |
author_sort | Fan, Huacai |
collection | PubMed |
description | Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of Chujaibacter, Bacillus, and Sphingomonas were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen (Foc TR4) content showed that Bacillus was significantly negatively correlated with pathogen content. Therefore, we isolated and identified Bacillus from the disease-suppressive soils, and obtained a B. velezensis strain YN1910. In vitro and pot experiments showed that YN1910 had a significant control effect (78.43–81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants. |
format | Online Article Text |
id | pubmed-10437119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104371192023-08-19 Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana Fan, Huacai He, Ping Xu, Shengtao Li, Shu Wang, Yongfen Zhang, Wenlong Li, Xundong Shang, Hui Zeng, Li Zheng, Si-Jun Front Microbiol Microbiology Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of Chujaibacter, Bacillus, and Sphingomonas were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen (Foc TR4) content showed that Bacillus was significantly negatively correlated with pathogen content. Therefore, we isolated and identified Bacillus from the disease-suppressive soils, and obtained a B. velezensis strain YN1910. In vitro and pot experiments showed that YN1910 had a significant control effect (78.43–81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants. Frontiers Media S.A. 2023-08-03 /pmc/articles/PMC10437119/ /pubmed/37601384 http://dx.doi.org/10.3389/fmicb.2023.1211301 Text en Copyright © 2023 Fan, He, Xu, Li, Wang, Zhang, Li, Shang, Zeng and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Fan, Huacai He, Ping Xu, Shengtao Li, Shu Wang, Yongfen Zhang, Wenlong Li, Xundong Shang, Hui Zeng, Li Zheng, Si-Jun Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana |
title | Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana |
title_full | Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana |
title_fullStr | Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana |
title_full_unstemmed | Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana |
title_short | Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana |
title_sort | banana disease-suppressive soil drives bacillus assembled to defense fusarium wilt of banana |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10437119/ https://www.ncbi.nlm.nih.gov/pubmed/37601384 http://dx.doi.org/10.3389/fmicb.2023.1211301 |
work_keys_str_mv | AT fanhuacai bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT heping bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT xushengtao bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT lishu bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT wangyongfen bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT zhangwenlong bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT lixundong bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT shanghui bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT zengli bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana AT zhengsijun bananadiseasesuppressivesoildrivesbacillusassembledtodefensefusariumwiltofbanana |