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Parallel genetic adaptation of Bacillus subtilis to different plant species

Plant growth-promoting rhizobacteria benefit plants by stimulating their growth or protecting them against phytopathogens. Rhizobacteria must colonize and persist on plant roots to exert their benefits. However, little is known regarding the processes by which rhizobacteria adapt to different plant...

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Autores principales: Hu, Guohai, Wang, Yue, Blake, Christopher, Nordgaard, Mathilde, Liu, Xin, Wang, Bo, Kovács, Ákos T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10438812/
https://www.ncbi.nlm.nih.gov/pubmed/37466402
http://dx.doi.org/10.1099/mgen.0.001064
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author Hu, Guohai
Wang, Yue
Blake, Christopher
Nordgaard, Mathilde
Liu, Xin
Wang, Bo
Kovács, Ákos T.
author_facet Hu, Guohai
Wang, Yue
Blake, Christopher
Nordgaard, Mathilde
Liu, Xin
Wang, Bo
Kovács, Ákos T.
author_sort Hu, Guohai
collection PubMed
description Plant growth-promoting rhizobacteria benefit plants by stimulating their growth or protecting them against phytopathogens. Rhizobacteria must colonize and persist on plant roots to exert their benefits. However, little is known regarding the processes by which rhizobacteria adapt to different plant species, or behave under alternating host plant regimes. Here, we used experimental evolution and whole-population whole-genome sequencing to analyse how Bacillus subtilis evolves on Arabidopsis thaliana and tomato seedlings, and under an alternating host plant regime, in a static hydroponic setup. We observed parallel evolution across multiple levels of biological organization in all conditions, which was greatest for the two heterogeneous, multi-resource, spatially structured environments at the genetic level. Species-specific adaptation at the genetic level was also observed, possibly caused by the selection stress imposed by different host plants. Furthermore, a trade-off between motility and biofilm development was supported by mutational changes in motility- and biofilm-related genes. Finally, we identified several condition-specific and common targeted genes in different environments by comparing three different B. subtilis biofilm adaptation settings. The results demonstrate a common evolutionary pattern when B. subtilis is adapting to the plant rhizosphere in similar conditions, and reveal differences in genetic mechanisms between different host plants. These findings will likely support strain improvements for sustainable agriculture.
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spelling pubmed-104388122023-08-19 Parallel genetic adaptation of Bacillus subtilis to different plant species Hu, Guohai Wang, Yue Blake, Christopher Nordgaard, Mathilde Liu, Xin Wang, Bo Kovács, Ákos T. Microb Genom Research Articles Plant growth-promoting rhizobacteria benefit plants by stimulating their growth or protecting them against phytopathogens. Rhizobacteria must colonize and persist on plant roots to exert their benefits. However, little is known regarding the processes by which rhizobacteria adapt to different plant species, or behave under alternating host plant regimes. Here, we used experimental evolution and whole-population whole-genome sequencing to analyse how Bacillus subtilis evolves on Arabidopsis thaliana and tomato seedlings, and under an alternating host plant regime, in a static hydroponic setup. We observed parallel evolution across multiple levels of biological organization in all conditions, which was greatest for the two heterogeneous, multi-resource, spatially structured environments at the genetic level. Species-specific adaptation at the genetic level was also observed, possibly caused by the selection stress imposed by different host plants. Furthermore, a trade-off between motility and biofilm development was supported by mutational changes in motility- and biofilm-related genes. Finally, we identified several condition-specific and common targeted genes in different environments by comparing three different B. subtilis biofilm adaptation settings. The results demonstrate a common evolutionary pattern when B. subtilis is adapting to the plant rhizosphere in similar conditions, and reveal differences in genetic mechanisms between different host plants. These findings will likely support strain improvements for sustainable agriculture. Microbiology Society 2023-07-19 /pmc/articles/PMC10438812/ /pubmed/37466402 http://dx.doi.org/10.1099/mgen.0.001064 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Articles
Hu, Guohai
Wang, Yue
Blake, Christopher
Nordgaard, Mathilde
Liu, Xin
Wang, Bo
Kovács, Ákos T.
Parallel genetic adaptation of Bacillus subtilis to different plant species
title Parallel genetic adaptation of Bacillus subtilis to different plant species
title_full Parallel genetic adaptation of Bacillus subtilis to different plant species
title_fullStr Parallel genetic adaptation of Bacillus subtilis to different plant species
title_full_unstemmed Parallel genetic adaptation of Bacillus subtilis to different plant species
title_short Parallel genetic adaptation of Bacillus subtilis to different plant species
title_sort parallel genetic adaptation of bacillus subtilis to different plant species
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10438812/
https://www.ncbi.nlm.nih.gov/pubmed/37466402
http://dx.doi.org/10.1099/mgen.0.001064
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