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Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
Reef-building corals thrive in oligotrophic environments due to their possession of endosymbiotic algae. Confined to the low pH interior of the symbiosome within the cell, the algal symbiont provides the coral host with photosynthetically fixed carbon. However, it remains unknown how carbon is relea...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10438907/ https://www.ncbi.nlm.nih.gov/pubmed/37594171 http://dx.doi.org/10.7554/eLife.80628 |
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author | Ishii, Yuu Ishii, Hironori Kuroha, Takeshi Yokoyama, Ryusuke Deguchi, Ryusaku Nishitani, Kazuhiko Minagawa, Jun Kawata, Masakado Takahashi, Shunichi Maruyama, Shinichiro |
author_facet | Ishii, Yuu Ishii, Hironori Kuroha, Takeshi Yokoyama, Ryusuke Deguchi, Ryusaku Nishitani, Kazuhiko Minagawa, Jun Kawata, Masakado Takahashi, Shunichi Maruyama, Shinichiro |
author_sort | Ishii, Yuu |
collection | PubMed |
description | Reef-building corals thrive in oligotrophic environments due to their possession of endosymbiotic algae. Confined to the low pH interior of the symbiosome within the cell, the algal symbiont provides the coral host with photosynthetically fixed carbon. However, it remains unknown how carbon is released from the algal symbiont for uptake by the host. Here we show, using cultured symbiotic dinoflagellate, Breviolum sp., that decreases in pH directly accelerates the release of monosaccharides, that is, glucose and galactose, into the ambient environment. Under low pH conditions, the cell surface structures were deformed and genes related to cellulase were significantly upregulated in Breviolum. Importantly, the release of monosaccharides was suppressed by the cellulase inhibitor, glucopyranoside, linking the release of carbon to degradation of the agal cell wall. Our results suggest that the low pH signals the cellulase-mediated release of monosaccharides from the algal cell wall as an environmental response in coral reef ecosystems. |
format | Online Article Text |
id | pubmed-10438907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-104389072023-08-19 Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga Ishii, Yuu Ishii, Hironori Kuroha, Takeshi Yokoyama, Ryusuke Deguchi, Ryusaku Nishitani, Kazuhiko Minagawa, Jun Kawata, Masakado Takahashi, Shunichi Maruyama, Shinichiro eLife Microbiology and Infectious Disease Reef-building corals thrive in oligotrophic environments due to their possession of endosymbiotic algae. Confined to the low pH interior of the symbiosome within the cell, the algal symbiont provides the coral host with photosynthetically fixed carbon. However, it remains unknown how carbon is released from the algal symbiont for uptake by the host. Here we show, using cultured symbiotic dinoflagellate, Breviolum sp., that decreases in pH directly accelerates the release of monosaccharides, that is, glucose and galactose, into the ambient environment. Under low pH conditions, the cell surface structures were deformed and genes related to cellulase were significantly upregulated in Breviolum. Importantly, the release of monosaccharides was suppressed by the cellulase inhibitor, glucopyranoside, linking the release of carbon to degradation of the agal cell wall. Our results suggest that the low pH signals the cellulase-mediated release of monosaccharides from the algal cell wall as an environmental response in coral reef ecosystems. eLife Sciences Publications, Ltd 2023-08-18 /pmc/articles/PMC10438907/ /pubmed/37594171 http://dx.doi.org/10.7554/eLife.80628 Text en © 2023, Ishii et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Ishii, Yuu Ishii, Hironori Kuroha, Takeshi Yokoyama, Ryusuke Deguchi, Ryusaku Nishitani, Kazuhiko Minagawa, Jun Kawata, Masakado Takahashi, Shunichi Maruyama, Shinichiro Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga |
title | Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga |
title_full | Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga |
title_fullStr | Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga |
title_full_unstemmed | Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga |
title_short | Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga |
title_sort | environmental ph signals the release of monosaccharides from cell wall in coral symbiotic alga |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10438907/ https://www.ncbi.nlm.nih.gov/pubmed/37594171 http://dx.doi.org/10.7554/eLife.80628 |
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