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Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga

Reef-building corals thrive in oligotrophic environments due to their possession of endosymbiotic algae. Confined to the low pH interior of the symbiosome within the cell, the algal symbiont provides the coral host with photosynthetically fixed carbon. However, it remains unknown how carbon is relea...

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Autores principales: Ishii, Yuu, Ishii, Hironori, Kuroha, Takeshi, Yokoyama, Ryusuke, Deguchi, Ryusaku, Nishitani, Kazuhiko, Minagawa, Jun, Kawata, Masakado, Takahashi, Shunichi, Maruyama, Shinichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10438907/
https://www.ncbi.nlm.nih.gov/pubmed/37594171
http://dx.doi.org/10.7554/eLife.80628
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author Ishii, Yuu
Ishii, Hironori
Kuroha, Takeshi
Yokoyama, Ryusuke
Deguchi, Ryusaku
Nishitani, Kazuhiko
Minagawa, Jun
Kawata, Masakado
Takahashi, Shunichi
Maruyama, Shinichiro
author_facet Ishii, Yuu
Ishii, Hironori
Kuroha, Takeshi
Yokoyama, Ryusuke
Deguchi, Ryusaku
Nishitani, Kazuhiko
Minagawa, Jun
Kawata, Masakado
Takahashi, Shunichi
Maruyama, Shinichiro
author_sort Ishii, Yuu
collection PubMed
description Reef-building corals thrive in oligotrophic environments due to their possession of endosymbiotic algae. Confined to the low pH interior of the symbiosome within the cell, the algal symbiont provides the coral host with photosynthetically fixed carbon. However, it remains unknown how carbon is released from the algal symbiont for uptake by the host. Here we show, using cultured symbiotic dinoflagellate, Breviolum sp., that decreases in pH directly accelerates the release of monosaccharides, that is, glucose and galactose, into the ambient environment. Under low pH conditions, the cell surface structures were deformed and genes related to cellulase were significantly upregulated in Breviolum. Importantly, the release of monosaccharides was suppressed by the cellulase inhibitor, glucopyranoside, linking the release of carbon to degradation of the agal cell wall. Our results suggest that the low pH signals the cellulase-mediated release of monosaccharides from the algal cell wall as an environmental response in coral reef ecosystems.
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spelling pubmed-104389072023-08-19 Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga Ishii, Yuu Ishii, Hironori Kuroha, Takeshi Yokoyama, Ryusuke Deguchi, Ryusaku Nishitani, Kazuhiko Minagawa, Jun Kawata, Masakado Takahashi, Shunichi Maruyama, Shinichiro eLife Microbiology and Infectious Disease Reef-building corals thrive in oligotrophic environments due to their possession of endosymbiotic algae. Confined to the low pH interior of the symbiosome within the cell, the algal symbiont provides the coral host with photosynthetically fixed carbon. However, it remains unknown how carbon is released from the algal symbiont for uptake by the host. Here we show, using cultured symbiotic dinoflagellate, Breviolum sp., that decreases in pH directly accelerates the release of monosaccharides, that is, glucose and galactose, into the ambient environment. Under low pH conditions, the cell surface structures were deformed and genes related to cellulase were significantly upregulated in Breviolum. Importantly, the release of monosaccharides was suppressed by the cellulase inhibitor, glucopyranoside, linking the release of carbon to degradation of the agal cell wall. Our results suggest that the low pH signals the cellulase-mediated release of monosaccharides from the algal cell wall as an environmental response in coral reef ecosystems. eLife Sciences Publications, Ltd 2023-08-18 /pmc/articles/PMC10438907/ /pubmed/37594171 http://dx.doi.org/10.7554/eLife.80628 Text en © 2023, Ishii et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Ishii, Yuu
Ishii, Hironori
Kuroha, Takeshi
Yokoyama, Ryusuke
Deguchi, Ryusaku
Nishitani, Kazuhiko
Minagawa, Jun
Kawata, Masakado
Takahashi, Shunichi
Maruyama, Shinichiro
Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
title Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
title_full Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
title_fullStr Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
title_full_unstemmed Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
title_short Environmental pH signals the release of monosaccharides from cell wall in coral symbiotic alga
title_sort environmental ph signals the release of monosaccharides from cell wall in coral symbiotic alga
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10438907/
https://www.ncbi.nlm.nih.gov/pubmed/37594171
http://dx.doi.org/10.7554/eLife.80628
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