Cargando…

Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes

The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioav...

Descripción completa

Detalles Bibliográficos
Autores principales: Chylinski, Caroline, Degnes, Kristin Fløgstad, Aasen, Inga Marie, Ptochos, Sokratis, Blomstrand, Berit Marie, Mahnert, Karl-Christian, Enemark, Heidi Larsen, Thamsborg, Stig Milan, Steinshamn, Håvard, Athanasiadou, Spiridoula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439207/
https://www.ncbi.nlm.nih.gov/pubmed/37596334
http://dx.doi.org/10.1038/s41598-023-38476-0
_version_ 1785092895465799680
author Chylinski, Caroline
Degnes, Kristin Fløgstad
Aasen, Inga Marie
Ptochos, Sokratis
Blomstrand, Berit Marie
Mahnert, Karl-Christian
Enemark, Heidi Larsen
Thamsborg, Stig Milan
Steinshamn, Håvard
Athanasiadou, Spiridoula
author_facet Chylinski, Caroline
Degnes, Kristin Fløgstad
Aasen, Inga Marie
Ptochos, Sokratis
Blomstrand, Berit Marie
Mahnert, Karl-Christian
Enemark, Heidi Larsen
Thamsborg, Stig Milan
Steinshamn, Håvard
Athanasiadou, Spiridoula
author_sort Chylinski, Caroline
collection PubMed
description The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock.
format Online
Article
Text
id pubmed-10439207
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-104392072023-08-20 Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes Chylinski, Caroline Degnes, Kristin Fløgstad Aasen, Inga Marie Ptochos, Sokratis Blomstrand, Berit Marie Mahnert, Karl-Christian Enemark, Heidi Larsen Thamsborg, Stig Milan Steinshamn, Håvard Athanasiadou, Spiridoula Sci Rep Article The antiparasitic potential of plants could offer a vital solution to alleviating the costs of gastrointestinal nematode (GIN) infections in ruminant production globally. Leveraging known bioactive molecules, however, is complex, where plant species, extraction processes and seasonality impact bioavailability and efficacy. This study assessed the impact of a comprehensive set of factors on the antiparasitic activity of Norwegian conifers to identify bark compounds specific against GIN. Antiparasitic activity was determined using in vitro assays targeting morphologically distinct life stages of ovine GIN: the egg hatch assay and larval motility assay. In depth characterisation of the chemical composition of the bark extracts was carried out using chromatographic separation, UV-absorbance, and molecular mass profiles to identify compounds implicated in the activity. Three key findings emerged: (1) the activity of bark extracts varied markedly from 0 to 100% antiparasitic efficacy, owing to tree species, extraction solvent and seasonality; (2) the GIN exhibited species-and stage-specific susceptibility to the bark extracts; (3) the presence of condensed tannins, amongst other compounds, was associated with anthelmintic activity. These findings add new insights into urgently needed alternative parasite control strategies in livestock. Nature Publishing Group UK 2023-08-18 /pmc/articles/PMC10439207/ /pubmed/37596334 http://dx.doi.org/10.1038/s41598-023-38476-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chylinski, Caroline
Degnes, Kristin Fløgstad
Aasen, Inga Marie
Ptochos, Sokratis
Blomstrand, Berit Marie
Mahnert, Karl-Christian
Enemark, Heidi Larsen
Thamsborg, Stig Milan
Steinshamn, Håvard
Athanasiadou, Spiridoula
Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes
title Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes
title_full Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes
title_fullStr Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes
title_full_unstemmed Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes
title_short Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes
title_sort condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of nordic conifer bark against gastrointestinal nematodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439207/
https://www.ncbi.nlm.nih.gov/pubmed/37596334
http://dx.doi.org/10.1038/s41598-023-38476-0
work_keys_str_mv AT chylinskicaroline condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT degneskristinfløgstad condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT aaseningamarie condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT ptochossokratis condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT blomstrandberitmarie condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT mahnertkarlchristian condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT enemarkheidilarsen condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT thamsborgstigmilan condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT steinshamnhavard condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes
AT athanasiadouspiridoula condensedtanninsnovelcompoundsandsourcesofvariationdeterminetheantiparasiticactivityofnordicconiferbarkagainstgastrointestinalnematodes