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mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals

SARS-CoV-2 infection and mRNA vaccination both elicit spike (S)-specific T cell responses. To analyze how T cell memory from prior infection influences T cell responses to vaccination, we evaluated functional T cell responses in naive and previously infected vaccine recipients. Pre-vaccine S-specifi...

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Autores principales: Mayer-Blackwell, Koshlan, Ryu, Heeju, Codd, Amy S., Parks, K. Rachael, MacMillan, Hugh R., Cohen, Kristen W., Stewart, Terri L., Seese, Aaron, Lemos, Maria P., De Rosa, Stephen C., Czartoski, Julie L., Moodie, Zoe, Nguyen, Long T., McGuire, Donald J., Ahmed, Rafi, Fiore-Gartland, Andrew, McElrath, M. Juliana, Newell, Evan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439252/
https://www.ncbi.nlm.nih.gov/pubmed/37552991
http://dx.doi.org/10.1016/j.xcrm.2023.101149
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author Mayer-Blackwell, Koshlan
Ryu, Heeju
Codd, Amy S.
Parks, K. Rachael
MacMillan, Hugh R.
Cohen, Kristen W.
Stewart, Terri L.
Seese, Aaron
Lemos, Maria P.
De Rosa, Stephen C.
Czartoski, Julie L.
Moodie, Zoe
Nguyen, Long T.
McGuire, Donald J.
Ahmed, Rafi
Fiore-Gartland, Andrew
McElrath, M. Juliana
Newell, Evan W.
author_facet Mayer-Blackwell, Koshlan
Ryu, Heeju
Codd, Amy S.
Parks, K. Rachael
MacMillan, Hugh R.
Cohen, Kristen W.
Stewart, Terri L.
Seese, Aaron
Lemos, Maria P.
De Rosa, Stephen C.
Czartoski, Julie L.
Moodie, Zoe
Nguyen, Long T.
McGuire, Donald J.
Ahmed, Rafi
Fiore-Gartland, Andrew
McElrath, M. Juliana
Newell, Evan W.
author_sort Mayer-Blackwell, Koshlan
collection PubMed
description SARS-CoV-2 infection and mRNA vaccination both elicit spike (S)-specific T cell responses. To analyze how T cell memory from prior infection influences T cell responses to vaccination, we evaluated functional T cell responses in naive and previously infected vaccine recipients. Pre-vaccine S-specific responses are predictive of subsequent CD8(+) T cell vaccine-response magnitudes. Comparing baseline with post-vaccination TCRβ repertoires, we observed large clonotypic expansions correlated with the frequency of spike-specific T cells. Epitope mapping the largest CD8(+) T cell responses confirms that an HLA-A∗03:01 epitope was highly immunodominant. Peptide-MHC tetramer staining together with mass cytometry and single-cell sequencing permit detailed phenotyping and clonotypic tracking of these S-specific CD8(+) T cells. Our results demonstrate that infection-induced S-specific CD8(+) T cell memory plays a significant role in shaping the magnitude and clonal composition of the circulating T cell repertoire after vaccination, with mRNA vaccination promoting CD8(+) memory T cells to a T(EMRA)-like phenotype.
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spelling pubmed-104392522023-08-20 mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals Mayer-Blackwell, Koshlan Ryu, Heeju Codd, Amy S. Parks, K. Rachael MacMillan, Hugh R. Cohen, Kristen W. Stewart, Terri L. Seese, Aaron Lemos, Maria P. De Rosa, Stephen C. Czartoski, Julie L. Moodie, Zoe Nguyen, Long T. McGuire, Donald J. Ahmed, Rafi Fiore-Gartland, Andrew McElrath, M. Juliana Newell, Evan W. Cell Rep Med Article SARS-CoV-2 infection and mRNA vaccination both elicit spike (S)-specific T cell responses. To analyze how T cell memory from prior infection influences T cell responses to vaccination, we evaluated functional T cell responses in naive and previously infected vaccine recipients. Pre-vaccine S-specific responses are predictive of subsequent CD8(+) T cell vaccine-response magnitudes. Comparing baseline with post-vaccination TCRβ repertoires, we observed large clonotypic expansions correlated with the frequency of spike-specific T cells. Epitope mapping the largest CD8(+) T cell responses confirms that an HLA-A∗03:01 epitope was highly immunodominant. Peptide-MHC tetramer staining together with mass cytometry and single-cell sequencing permit detailed phenotyping and clonotypic tracking of these S-specific CD8(+) T cells. Our results demonstrate that infection-induced S-specific CD8(+) T cell memory plays a significant role in shaping the magnitude and clonal composition of the circulating T cell repertoire after vaccination, with mRNA vaccination promoting CD8(+) memory T cells to a T(EMRA)-like phenotype. Elsevier 2023-08-07 /pmc/articles/PMC10439252/ /pubmed/37552991 http://dx.doi.org/10.1016/j.xcrm.2023.101149 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Mayer-Blackwell, Koshlan
Ryu, Heeju
Codd, Amy S.
Parks, K. Rachael
MacMillan, Hugh R.
Cohen, Kristen W.
Stewart, Terri L.
Seese, Aaron
Lemos, Maria P.
De Rosa, Stephen C.
Czartoski, Julie L.
Moodie, Zoe
Nguyen, Long T.
McGuire, Donald J.
Ahmed, Rafi
Fiore-Gartland, Andrew
McElrath, M. Juliana
Newell, Evan W.
mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals
title mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals
title_full mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals
title_fullStr mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals
title_full_unstemmed mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals
title_short mRNA vaccination boosts S-specific T cell memory and promotes expansion of CD45RA(int) T(EMRA)-like CD8(+) T cells in COVID-19 recovered individuals
title_sort mrna vaccination boosts s-specific t cell memory and promotes expansion of cd45ra(int) t(emra)-like cd8(+) t cells in covid-19 recovered individuals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439252/
https://www.ncbi.nlm.nih.gov/pubmed/37552991
http://dx.doi.org/10.1016/j.xcrm.2023.101149
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