A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance

BACKGROUND: Phloem protein 2 (PP2) proteins play a vital role in the Phloem-based defense (PBD) and participate in many abiotic and biotic stress. However, research on PP2 proteins in cotton is still lacking. RESULTS: A total of 25, 23, 43, and 47 PP2 genes were comprehensively identified and charac...

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Autores principales: Wei, Fei, Chen, Pengyun, Jian, Hongliang, Guo, Xiaohao, Lv, Xiaoyan, Lian, Boying, Sun, Mengxi, An, Li, Dang, Xinyu, Yang, Miaoqian, Wu, Hongmei, Zhang, Nan, Wu, Aimin, Wang, Hantao, Ma, Liang, Fu, Xiaokang, Lu, Jianhua, Yu, Shuxun, Wei, Hengling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439568/
https://www.ncbi.nlm.nih.gov/pubmed/37596513
http://dx.doi.org/10.1186/s12864-023-09546-4
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author Wei, Fei
Chen, Pengyun
Jian, Hongliang
Guo, Xiaohao
Lv, Xiaoyan
Lian, Boying
Sun, Mengxi
An, Li
Dang, Xinyu
Yang, Miaoqian
Wu, Hongmei
Zhang, Nan
Wu, Aimin
Wang, Hantao
Ma, Liang
Fu, Xiaokang
Lu, Jianhua
Yu, Shuxun
Wei, Hengling
author_facet Wei, Fei
Chen, Pengyun
Jian, Hongliang
Guo, Xiaohao
Lv, Xiaoyan
Lian, Boying
Sun, Mengxi
An, Li
Dang, Xinyu
Yang, Miaoqian
Wu, Hongmei
Zhang, Nan
Wu, Aimin
Wang, Hantao
Ma, Liang
Fu, Xiaokang
Lu, Jianhua
Yu, Shuxun
Wei, Hengling
author_sort Wei, Fei
collection PubMed
description BACKGROUND: Phloem protein 2 (PP2) proteins play a vital role in the Phloem-based defense (PBD) and participate in many abiotic and biotic stress. However, research on PP2 proteins in cotton is still lacking. RESULTS: A total of 25, 23, 43, and 47 PP2 genes were comprehensively identified and characterized in G.arboretum, G.raimondii, G.barbadense, and G.hirsutum. The whole genome duplication (WGD) and allopolyploidization events play essential roles in the expansion of PP2 genes. The promoter regions of GhPP2 genes contain many cis-acting elements related to abiotic stress and the weighted gene co-expression network analysis (WGCNA) analysis displayed that GhPP2s could be related to salt stress. The qRT-PCR assays further confirmed that GhPP2-33 could be dramatically upregulated during the salt treatment. And the virus-induced gene silencing (VIGS) experiment proved that the silencing of GhPP2-33 could decrease salt tolerance. CONCLUSIONS: The results in this study not only offer new perspectives for understanding the evolution of PP2 genes in cotton but also further explore their function under salt stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09546-4.
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spelling pubmed-104395682023-08-20 A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance Wei, Fei Chen, Pengyun Jian, Hongliang Guo, Xiaohao Lv, Xiaoyan Lian, Boying Sun, Mengxi An, Li Dang, Xinyu Yang, Miaoqian Wu, Hongmei Zhang, Nan Wu, Aimin Wang, Hantao Ma, Liang Fu, Xiaokang Lu, Jianhua Yu, Shuxun Wei, Hengling BMC Genomics Research BACKGROUND: Phloem protein 2 (PP2) proteins play a vital role in the Phloem-based defense (PBD) and participate in many abiotic and biotic stress. However, research on PP2 proteins in cotton is still lacking. RESULTS: A total of 25, 23, 43, and 47 PP2 genes were comprehensively identified and characterized in G.arboretum, G.raimondii, G.barbadense, and G.hirsutum. The whole genome duplication (WGD) and allopolyploidization events play essential roles in the expansion of PP2 genes. The promoter regions of GhPP2 genes contain many cis-acting elements related to abiotic stress and the weighted gene co-expression network analysis (WGCNA) analysis displayed that GhPP2s could be related to salt stress. The qRT-PCR assays further confirmed that GhPP2-33 could be dramatically upregulated during the salt treatment. And the virus-induced gene silencing (VIGS) experiment proved that the silencing of GhPP2-33 could decrease salt tolerance. CONCLUSIONS: The results in this study not only offer new perspectives for understanding the evolution of PP2 genes in cotton but also further explore their function under salt stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09546-4. BioMed Central 2023-08-18 /pmc/articles/PMC10439568/ /pubmed/37596513 http://dx.doi.org/10.1186/s12864-023-09546-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wei, Fei
Chen, Pengyun
Jian, Hongliang
Guo, Xiaohao
Lv, Xiaoyan
Lian, Boying
Sun, Mengxi
An, Li
Dang, Xinyu
Yang, Miaoqian
Wu, Hongmei
Zhang, Nan
Wu, Aimin
Wang, Hantao
Ma, Liang
Fu, Xiaokang
Lu, Jianhua
Yu, Shuxun
Wei, Hengling
A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance
title A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance
title_full A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance
title_fullStr A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance
title_full_unstemmed A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance
title_short A systematic analysis of the phloem protein 2 (PP2) proteins in Gossypium hirsutum reveals that GhPP2-33 regulates salt tolerance
title_sort systematic analysis of the phloem protein 2 (pp2) proteins in gossypium hirsutum reveals that ghpp2-33 regulates salt tolerance
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439568/
https://www.ncbi.nlm.nih.gov/pubmed/37596513
http://dx.doi.org/10.1186/s12864-023-09546-4
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