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Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate

BACKGROUND: The coexistence of bla(NDM-1) with other resistance determinants is rarely reported for Providencia rettgeri. Therefore, this study investigates the phenotypic and genetic characteristics of a multidrug-resistant P. rettgeri strain YQ150713. METHODS: P. rettgeri YQ150713 was identified a...

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Autores principales: Li, Yaling, Shao, Kaiyang, Cai, Ruyi, Liu, Yi, Liu, Xiaojing, Ni, Feihua, Zheng, Huiyan, Hu, Ruying, Sun, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439778/
https://www.ncbi.nlm.nih.gov/pubmed/37601562
http://dx.doi.org/10.2147/IDR.S418131
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author Li, Yaling
Shao, Kaiyang
Cai, Ruyi
Liu, Yi
Liu, Xiaojing
Ni, Feihua
Zheng, Huiyan
Hu, Ruying
Sun, Ting
author_facet Li, Yaling
Shao, Kaiyang
Cai, Ruyi
Liu, Yi
Liu, Xiaojing
Ni, Feihua
Zheng, Huiyan
Hu, Ruying
Sun, Ting
author_sort Li, Yaling
collection PubMed
description BACKGROUND: The coexistence of bla(NDM-1) with other resistance determinants is rarely reported for Providencia rettgeri. Therefore, this study investigates the phenotypic and genetic characteristics of a multidrug-resistant P. rettgeri strain YQ150713. METHODS: P. rettgeri YQ150713 was identified as carrying bla(NDM-1). S1-pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, and conjugation experiments were used to determine plasmid characteristics. An antimicrobial susceptibility test was conducted. The complete genomic sequence of YQ150713 was obtained using Illumina NovaSeq 6000 and Oxford nanopore platforms. To further characterize the phylogenetic structure of P. rettgeri YQ150713, average nucleotide identity (ANI) and phylogenetic analyses were conducted. RESULTS: The S1-PFGE, Southern blot, and conjugation assays have confirmed that the isolate P. rettgeri YQ150713 contains the bla(NDM-1) gene on a conjugative plasmid pYQ150713-NDM-1. Antimicrobial susceptibility testing has indicated that strain YQ150713 was resistant to various common antibiotics, except aztreonam and fosfomycin. Bioinformatics analysis has further shown that pYQ150713-NDM-1 was a novel plasmid with a size of 265,883 bp, and bla(NDM-1) and bla(OXA-10) were co-located on it. Phylogenetic analysis suggesting P. rettgeri has spread widely throughout the world. CONCLUSION: In this study, bla(NDM-1) and bla(OXA-10) were co-localized on a novel plasmid pYQ150713-NDM-1 with a horizontal transfer function. To reduce the risk of the dissemination of such P. rettgeri isolates in clinical settings, more surveillance will be required in the future.
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spelling pubmed-104397782023-08-20 Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate Li, Yaling Shao, Kaiyang Cai, Ruyi Liu, Yi Liu, Xiaojing Ni, Feihua Zheng, Huiyan Hu, Ruying Sun, Ting Infect Drug Resist Short Report BACKGROUND: The coexistence of bla(NDM-1) with other resistance determinants is rarely reported for Providencia rettgeri. Therefore, this study investigates the phenotypic and genetic characteristics of a multidrug-resistant P. rettgeri strain YQ150713. METHODS: P. rettgeri YQ150713 was identified as carrying bla(NDM-1). S1-pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, and conjugation experiments were used to determine plasmid characteristics. An antimicrobial susceptibility test was conducted. The complete genomic sequence of YQ150713 was obtained using Illumina NovaSeq 6000 and Oxford nanopore platforms. To further characterize the phylogenetic structure of P. rettgeri YQ150713, average nucleotide identity (ANI) and phylogenetic analyses were conducted. RESULTS: The S1-PFGE, Southern blot, and conjugation assays have confirmed that the isolate P. rettgeri YQ150713 contains the bla(NDM-1) gene on a conjugative plasmid pYQ150713-NDM-1. Antimicrobial susceptibility testing has indicated that strain YQ150713 was resistant to various common antibiotics, except aztreonam and fosfomycin. Bioinformatics analysis has further shown that pYQ150713-NDM-1 was a novel plasmid with a size of 265,883 bp, and bla(NDM-1) and bla(OXA-10) were co-located on it. Phylogenetic analysis suggesting P. rettgeri has spread widely throughout the world. CONCLUSION: In this study, bla(NDM-1) and bla(OXA-10) were co-localized on a novel plasmid pYQ150713-NDM-1 with a horizontal transfer function. To reduce the risk of the dissemination of such P. rettgeri isolates in clinical settings, more surveillance will be required in the future. Dove 2023-08-15 /pmc/articles/PMC10439778/ /pubmed/37601562 http://dx.doi.org/10.2147/IDR.S418131 Text en © 2023 Li et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Short Report
Li, Yaling
Shao, Kaiyang
Cai, Ruyi
Liu, Yi
Liu, Xiaojing
Ni, Feihua
Zheng, Huiyan
Hu, Ruying
Sun, Ting
Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate
title Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate
title_full Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate
title_fullStr Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate
title_full_unstemmed Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate
title_short Detection of NDM-1 and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate
title_sort detection of ndm-1 and oxa-10 co-producing providencia rettgeri clinical isolate
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439778/
https://www.ncbi.nlm.nih.gov/pubmed/37601562
http://dx.doi.org/10.2147/IDR.S418131
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