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Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Trichosporon asahii is an opportunistic pathogenic fungus that causes severe and sometimes fatal infections in immunocompromised patients. Hog1, a mitogen-activated protein kinase, regulates the stress resistance of some pathogenic fungi, however its role in T. asahii has not been investigated. Here...

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Autores principales: Matsumoto, Yasuhiko, Sugiyama, Yu, Nagamachi, Tae, Yoshikawa, Asami, Sugita, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439922/
https://www.ncbi.nlm.nih.gov/pubmed/37598230
http://dx.doi.org/10.1038/s41598-023-40825-y
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author Matsumoto, Yasuhiko
Sugiyama, Yu
Nagamachi, Tae
Yoshikawa, Asami
Sugita, Takashi
author_facet Matsumoto, Yasuhiko
Sugiyama, Yu
Nagamachi, Tae
Yoshikawa, Asami
Sugita, Takashi
author_sort Matsumoto, Yasuhiko
collection PubMed
description Trichosporon asahii is an opportunistic pathogenic fungus that causes severe and sometimes fatal infections in immunocompromised patients. Hog1, a mitogen-activated protein kinase, regulates the stress resistance of some pathogenic fungi, however its role in T. asahii has not been investigated. Here, we demonstrated that the hog1 gene-deficient T. asahii mutant is sensitive to high temperature, cell membrane stress, oxidative stress, and antifungal drugs. Growth of the hog1 gene-deficient T. asahii mutant was delayed at 40 °C. The hog1 gene-deficient T. asahii mutant also exhibited sensitivity to sodium dodecyl sulfate, hydrogen peroxide, menadione, methyl methanesulfonate, UV exposure, and antifungal drugs such as amphotericin B under a glucose-rich condition. Under a glucose-restricted condition, the hog1 gene-deficient mutant exhibited sensitivity to NaCl and KCl. The virulence of the hog1 gene-deficient mutant against silkworms was attenuated. Moreover, the viability of the hog1 gene-deficient mutant decreased in the silkworm hemolymph. These phenotypes were restored by re-introducing the hog1 gene into the gene-deficient mutant. Our findings suggest that Hog1 plays a critical role in regulating cellular stress responses in T. asahii.
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spelling pubmed-104399222023-08-21 Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii Matsumoto, Yasuhiko Sugiyama, Yu Nagamachi, Tae Yoshikawa, Asami Sugita, Takashi Sci Rep Article Trichosporon asahii is an opportunistic pathogenic fungus that causes severe and sometimes fatal infections in immunocompromised patients. Hog1, a mitogen-activated protein kinase, regulates the stress resistance of some pathogenic fungi, however its role in T. asahii has not been investigated. Here, we demonstrated that the hog1 gene-deficient T. asahii mutant is sensitive to high temperature, cell membrane stress, oxidative stress, and antifungal drugs. Growth of the hog1 gene-deficient T. asahii mutant was delayed at 40 °C. The hog1 gene-deficient T. asahii mutant also exhibited sensitivity to sodium dodecyl sulfate, hydrogen peroxide, menadione, methyl methanesulfonate, UV exposure, and antifungal drugs such as amphotericin B under a glucose-rich condition. Under a glucose-restricted condition, the hog1 gene-deficient mutant exhibited sensitivity to NaCl and KCl. The virulence of the hog1 gene-deficient mutant against silkworms was attenuated. Moreover, the viability of the hog1 gene-deficient mutant decreased in the silkworm hemolymph. These phenotypes were restored by re-introducing the hog1 gene into the gene-deficient mutant. Our findings suggest that Hog1 plays a critical role in regulating cellular stress responses in T. asahii. Nature Publishing Group UK 2023-08-19 /pmc/articles/PMC10439922/ /pubmed/37598230 http://dx.doi.org/10.1038/s41598-023-40825-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Matsumoto, Yasuhiko
Sugiyama, Yu
Nagamachi, Tae
Yoshikawa, Asami
Sugita, Takashi
Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
title Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
title_full Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
title_fullStr Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
title_full_unstemmed Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
title_short Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
title_sort hog1-mediated stress tolerance in the pathogenic fungus trichosporon asahii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439922/
https://www.ncbi.nlm.nih.gov/pubmed/37598230
http://dx.doi.org/10.1038/s41598-023-40825-y
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