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Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila

RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution micro...

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Autores principales: Curnutte, Harrison A., Lan, Xinyue, Sargen, Manuel, Ao Ieong, Si Man, Campbell, Dylan, Kim, Hyosik, Liao, Yijun, Lazar, Sarah Bailah, Trcek, Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439980/
https://www.ncbi.nlm.nih.gov/pubmed/37384531
http://dx.doi.org/10.1016/j.celrep.2023.112723
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author Curnutte, Harrison A.
Lan, Xinyue
Sargen, Manuel
Ao Ieong, Si Man
Campbell, Dylan
Kim, Hyosik
Liao, Yijun
Lazar, Sarah Bailah
Trcek, Tatjana
author_facet Curnutte, Harrison A.
Lan, Xinyue
Sargen, Manuel
Ao Ieong, Si Man
Campbell, Dylan
Kim, Hyosik
Liao, Yijun
Lazar, Sarah Bailah
Trcek, Tatjana
author_sort Curnutte, Harrison A.
collection PubMed
description RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies.
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spelling pubmed-104399802023-08-20 Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila Curnutte, Harrison A. Lan, Xinyue Sargen, Manuel Ao Ieong, Si Man Campbell, Dylan Kim, Hyosik Liao, Yijun Lazar, Sarah Bailah Trcek, Tatjana Cell Rep Article RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies. 2023-07-25 2023-06-28 /pmc/articles/PMC10439980/ /pubmed/37384531 http://dx.doi.org/10.1016/j.celrep.2023.112723 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Curnutte, Harrison A.
Lan, Xinyue
Sargen, Manuel
Ao Ieong, Si Man
Campbell, Dylan
Kim, Hyosik
Liao, Yijun
Lazar, Sarah Bailah
Trcek, Tatjana
Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
title Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
title_full Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
title_fullStr Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
title_full_unstemmed Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
title_short Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
title_sort proteins rather than mrnas regulate nucleation and persistence of oskar germ granules in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439980/
https://www.ncbi.nlm.nih.gov/pubmed/37384531
http://dx.doi.org/10.1016/j.celrep.2023.112723
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