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Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila
RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution micro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439980/ https://www.ncbi.nlm.nih.gov/pubmed/37384531 http://dx.doi.org/10.1016/j.celrep.2023.112723 |
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author | Curnutte, Harrison A. Lan, Xinyue Sargen, Manuel Ao Ieong, Si Man Campbell, Dylan Kim, Hyosik Liao, Yijun Lazar, Sarah Bailah Trcek, Tatjana |
author_facet | Curnutte, Harrison A. Lan, Xinyue Sargen, Manuel Ao Ieong, Si Man Campbell, Dylan Kim, Hyosik Liao, Yijun Lazar, Sarah Bailah Trcek, Tatjana |
author_sort | Curnutte, Harrison A. |
collection | PubMed |
description | RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies. |
format | Online Article Text |
id | pubmed-10439980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-104399802023-08-20 Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila Curnutte, Harrison A. Lan, Xinyue Sargen, Manuel Ao Ieong, Si Man Campbell, Dylan Kim, Hyosik Liao, Yijun Lazar, Sarah Bailah Trcek, Tatjana Cell Rep Article RNA granules are membraneless condensates that provide functional compartmentalization within cells. The mechanisms by which RNA granules form are under intense investigation. Here, we characterize the role of mRNAs and proteins in the formation of germ granules in Drosophila. Super-resolution microscopy reveals that the number, size, and distribution of germ granules is precisely controlled. Surprisingly, germ granule mRNAs are not required for the nucleation or the persistence of germ granules but instead control their size and composition. Using an RNAi screen, we determine that RNA regulators, helicases, and mitochondrial proteins regulate germ granule number and size, while the proteins of the endoplasmic reticulum, nuclear pore complex, and cytoskeleton control their distribution. Therefore, the protein-driven formation of Drosophila germ granules is mechanistically distinct from the RNA-dependent condensation observed for other RNA granules such as stress granules and P-bodies. 2023-07-25 2023-06-28 /pmc/articles/PMC10439980/ /pubmed/37384531 http://dx.doi.org/10.1016/j.celrep.2023.112723 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Curnutte, Harrison A. Lan, Xinyue Sargen, Manuel Ao Ieong, Si Man Campbell, Dylan Kim, Hyosik Liao, Yijun Lazar, Sarah Bailah Trcek, Tatjana Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
title | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
title_full | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
title_fullStr | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
title_full_unstemmed | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
title_short | Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila |
title_sort | proteins rather than mrnas regulate nucleation and persistence of oskar germ granules in drosophila |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439980/ https://www.ncbi.nlm.nih.gov/pubmed/37384531 http://dx.doi.org/10.1016/j.celrep.2023.112723 |
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