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Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations

We present a protocol for generating a precise deletion, without altering the genetic background of the strain, of a transposable element (TE) in a natural population of Drosophila melanogaster using two steps of CRISPR-Cas9 homology-directed repair. We describe steps for replacing the TE by a fluor...

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Detalles Bibliográficos
Autores principales:	Merenciano, Miriam, Aguilera, Laura, González, Josefa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440348/ https://www.ncbi.nlm.nih.gov/pubmed/37590151 http://dx.doi.org/10.1016/j.xpro.2023.102501

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