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Profiling and verifying the substrates of E3 ubiquitin ligase Rsp5 in yeast cells

Yeast is an essential model organism for studying protein ubiquitination pathways; however, identifying the direct substrates of E3 in the cell presents a challenge. Here, we present a protocol for using the orthogonal ubiquitin transfer (OUT) cascade to profile the substrate specificity of yeast E3...

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Detalles Bibliográficos
Autores principales:	Fang, Shuai, Chen, Geng, Wang, Yiyang, Ganti, Rakhee, Chernova, Tatiana A., Zhou, Li, Jacobs, Savannah E., Duong, Duc, Kiyokawa, Hiroaki, Chernoff, Yury O., Li, Ming, Shcherbik, Natalia, Zhao, Bo, Yin, Jun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440593/ https://www.ncbi.nlm.nih.gov/pubmed/37561636 http://dx.doi.org/10.1016/j.xpro.2023.102489

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