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Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives
Alkannin, shikonin and their derivatives (A/S) are secondary metabolites produced in the roots of certain plants of the Boraginaceae family such as Lithospermum erythrorhizon Siebold & Zucc. and Alkanna tinctoria (L.) Tausch. These naphthoquinones express anti-cancer, wound healing, and antimicr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440953/ https://www.ncbi.nlm.nih.gov/pubmed/37608899 http://dx.doi.org/10.3389/fphar.2023.1244270 |
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author | Rat, Angélique Koletti, Antigoni E. Rodić, Nebojša Papageorgiou, Vassilios P. Willems, Anne Assimopoulou, Andreana N. |
author_facet | Rat, Angélique Koletti, Antigoni E. Rodić, Nebojša Papageorgiou, Vassilios P. Willems, Anne Assimopoulou, Andreana N. |
author_sort | Rat, Angélique |
collection | PubMed |
description | Alkannin, shikonin and their derivatives (A/S) are secondary metabolites produced in the roots of certain plants of the Boraginaceae family such as Lithospermum erythrorhizon Siebold & Zucc. and Alkanna tinctoria (L.) Tausch. These naphthoquinones express anti-cancer, wound healing, and antimicrobial activities. To study the interactions between endophytic bacteria isolated from A. tinctoria and the antimicrobials A/S, endophytic bacteria known to be resistant to the compounds were screened for their effect on A/S in liquid medium. Thereafter, the strain Pseudomonas sp. R-72008, was selected and tested for its ability to modify A/S in nutrient medium and minimal medium with A/S as sole carbon source. Bacterial growth was recorded, and high performance liquid chromatography-diode array and ultra-high performance liquid chromatography-electrospray ionization-mass spectrometry analyses were performed to detect and quantify metabolites. In nutrient medium inoculated with R-72008, a decrease in the amount of A/S monomers initially present was observed and correlated with an increase of A/S oligomers. Moreover, a significant decrease of initial A/S monomers in minimal medium was correlated with bacterial growth, showing for the first time that a bacterial strain, Pseudomonas sp. R-72008, was able to use the naphthoquinones A/S as sole carbon source. This study opens new perspectives on the interactions between bacteria and plant antimicrobials. |
format | Online Article Text |
id | pubmed-10440953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104409532023-08-22 Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives Rat, Angélique Koletti, Antigoni E. Rodić, Nebojša Papageorgiou, Vassilios P. Willems, Anne Assimopoulou, Andreana N. Front Pharmacol Pharmacology Alkannin, shikonin and their derivatives (A/S) are secondary metabolites produced in the roots of certain plants of the Boraginaceae family such as Lithospermum erythrorhizon Siebold & Zucc. and Alkanna tinctoria (L.) Tausch. These naphthoquinones express anti-cancer, wound healing, and antimicrobial activities. To study the interactions between endophytic bacteria isolated from A. tinctoria and the antimicrobials A/S, endophytic bacteria known to be resistant to the compounds were screened for their effect on A/S in liquid medium. Thereafter, the strain Pseudomonas sp. R-72008, was selected and tested for its ability to modify A/S in nutrient medium and minimal medium with A/S as sole carbon source. Bacterial growth was recorded, and high performance liquid chromatography-diode array and ultra-high performance liquid chromatography-electrospray ionization-mass spectrometry analyses were performed to detect and quantify metabolites. In nutrient medium inoculated with R-72008, a decrease in the amount of A/S monomers initially present was observed and correlated with an increase of A/S oligomers. Moreover, a significant decrease of initial A/S monomers in minimal medium was correlated with bacterial growth, showing for the first time that a bacterial strain, Pseudomonas sp. R-72008, was able to use the naphthoquinones A/S as sole carbon source. This study opens new perspectives on the interactions between bacteria and plant antimicrobials. Frontiers Media S.A. 2023-08-07 /pmc/articles/PMC10440953/ /pubmed/37608899 http://dx.doi.org/10.3389/fphar.2023.1244270 Text en Copyright © 2023 Rat, Koletti, Rodić, Papageorgiou, Willems and Assimopoulou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Rat, Angélique Koletti, Antigoni E. Rodić, Nebojša Papageorgiou, Vassilios P. Willems, Anne Assimopoulou, Andreana N. Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
title | Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
title_full | Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
title_fullStr | Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
title_full_unstemmed | Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
title_short | Bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
title_sort | bacterial responses to plant antimicrobials: the case of alkannin and shikonin derivatives |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440953/ https://www.ncbi.nlm.nih.gov/pubmed/37608899 http://dx.doi.org/10.3389/fphar.2023.1244270 |
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