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Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement

Pennycress (Thlaspi arvense L.), a member of the Brassicaceae family, produces seed oil high in erucic acid, suitable for biodiesel and aviation fuel. Although pennycress, a winter annual, could be grown as a dedicated bioenergy crop, an increase in its seed oil content is required to improve its ec...

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Autores principales: Arias, Cintia Lucía, García Navarrete, Leidy Tatiana, Mukundi, Eric, Swanson, Tyler, Yang, Fan, Hernandez, Jonathan, Grotewold, Erich, Alonso, Ana Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440992/
https://www.ncbi.nlm.nih.gov/pubmed/37335591
http://dx.doi.org/10.1111/pbi.14101
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author Arias, Cintia Lucía
García Navarrete, Leidy Tatiana
Mukundi, Eric
Swanson, Tyler
Yang, Fan
Hernandez, Jonathan
Grotewold, Erich
Alonso, Ana Paula
author_facet Arias, Cintia Lucía
García Navarrete, Leidy Tatiana
Mukundi, Eric
Swanson, Tyler
Yang, Fan
Hernandez, Jonathan
Grotewold, Erich
Alonso, Ana Paula
author_sort Arias, Cintia Lucía
collection PubMed
description Pennycress (Thlaspi arvense L.), a member of the Brassicaceae family, produces seed oil high in erucic acid, suitable for biodiesel and aviation fuel. Although pennycress, a winter annual, could be grown as a dedicated bioenergy crop, an increase in its seed oil content is required to improve its economic competitiveness. The success of crop improvement relies upon finding the right combination of biomarkers and targets, and the best genetic engineering and/or breeding strategies. In this work, we combined biomass composition with metabolomic and transcriptomic studies of developing embryos from 22 pennycress natural variants to identify targets for oil improvement. The selected accession collection presented diverse levels of fatty acids at maturity ranging from 29% to 41%. Pearson correlation analyses, weighted gene co‐expression network analysis and biomarker identifications were used as complementary approaches to detect associations between metabolite level or gene expression and oil content at maturity. The results indicated that improving seed oil content can lead to a concomitant increase in the proportion of erucic acid without affecting the weight of embryos. Processes, such as carbon partitioning towards the chloroplast, lipid metabolism, photosynthesis, and a tight control of nitrogen availability, were found to be key for oil improvement in pennycress. Besides identifying specific targets, our results also provide guidance regarding the best timing for their modification, early or middle maturation. Thus, this work lays out promising strategies, specific for pennycress, to accelerate the successful development of lines with increased seed oil content for biofuel applications.
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spelling pubmed-104409922023-08-22 Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement Arias, Cintia Lucía García Navarrete, Leidy Tatiana Mukundi, Eric Swanson, Tyler Yang, Fan Hernandez, Jonathan Grotewold, Erich Alonso, Ana Paula Plant Biotechnol J Research Articles Pennycress (Thlaspi arvense L.), a member of the Brassicaceae family, produces seed oil high in erucic acid, suitable for biodiesel and aviation fuel. Although pennycress, a winter annual, could be grown as a dedicated bioenergy crop, an increase in its seed oil content is required to improve its economic competitiveness. The success of crop improvement relies upon finding the right combination of biomarkers and targets, and the best genetic engineering and/or breeding strategies. In this work, we combined biomass composition with metabolomic and transcriptomic studies of developing embryos from 22 pennycress natural variants to identify targets for oil improvement. The selected accession collection presented diverse levels of fatty acids at maturity ranging from 29% to 41%. Pearson correlation analyses, weighted gene co‐expression network analysis and biomarker identifications were used as complementary approaches to detect associations between metabolite level or gene expression and oil content at maturity. The results indicated that improving seed oil content can lead to a concomitant increase in the proportion of erucic acid without affecting the weight of embryos. Processes, such as carbon partitioning towards the chloroplast, lipid metabolism, photosynthesis, and a tight control of nitrogen availability, were found to be key for oil improvement in pennycress. Besides identifying specific targets, our results also provide guidance regarding the best timing for their modification, early or middle maturation. Thus, this work lays out promising strategies, specific for pennycress, to accelerate the successful development of lines with increased seed oil content for biofuel applications. John Wiley and Sons Inc. 2023-06-19 2023-09 /pmc/articles/PMC10440992/ /pubmed/37335591 http://dx.doi.org/10.1111/pbi.14101 Text en © 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Arias, Cintia Lucía
García Navarrete, Leidy Tatiana
Mukundi, Eric
Swanson, Tyler
Yang, Fan
Hernandez, Jonathan
Grotewold, Erich
Alonso, Ana Paula
Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
title Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
title_full Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
title_fullStr Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
title_full_unstemmed Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
title_short Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
title_sort metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440992/
https://www.ncbi.nlm.nih.gov/pubmed/37335591
http://dx.doi.org/10.1111/pbi.14101
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