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Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium
Introduction: Polysaccharides, key components present in Grifola frondosa, can be divided into those derived from fruiting bodies, mycelium, and fermentation broth based on their source. The structure of G. frondosa fruiting body-derived polysaccharides has been fully characterized. However, the str...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441114/ https://www.ncbi.nlm.nih.gov/pubmed/37608863 http://dx.doi.org/10.3389/fchem.2023.1227288 |
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author | Geng, Jie Wang, Guining Guo, Jiao Han, Xiao Qu, Yunhe Zhou, Yifa Tai, Guihua Sun, Lin Cheng, Hairong |
author_facet | Geng, Jie Wang, Guining Guo, Jiao Han, Xiao Qu, Yunhe Zhou, Yifa Tai, Guihua Sun, Lin Cheng, Hairong |
author_sort | Geng, Jie |
collection | PubMed |
description | Introduction: Polysaccharides, key components present in Grifola frondosa, can be divided into those derived from fruiting bodies, mycelium, and fermentation broth based on their source. The structure of G. frondosa fruiting body-derived polysaccharides has been fully characterized. However, the structure of G. frondosa mycelium-derived polysaccharides remains to be elucidated. Methods: In this study, we obtained mycelia from G. frondosa by liquid fermentation and extracted them with water and alkaline solution. Then, the mycelia were isolated and purified to obtain homogeneity and systematically characterized by methylation and FT infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy. Results and discussion: Structural analysis showed that two neutral fractions (WGFP-N-a and AGFP-N-a(1)) have a common backbone composed of α-1,6-D-Me-Galp and α-1,6-D-Galp that were substituted at O-2 by 1,2-Manp, α-1,3-L-Fucp, and α-T-D-Manp and thus are identified as fucomannogalactans. WGFP-A-a, AGFP-A-b, and AGFP-A-c are β-1,6-glucans with different molecular weights and are branched with β-1,3-D-Glcp and T-D-Glcp at the O-3 of Glc. Our results provide important structural information about G. frondosa mycelium-derived polysaccharides and provide the basis for their further development and application. |
format | Online Article Text |
id | pubmed-10441114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104411142023-08-22 Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium Geng, Jie Wang, Guining Guo, Jiao Han, Xiao Qu, Yunhe Zhou, Yifa Tai, Guihua Sun, Lin Cheng, Hairong Front Chem Chemistry Introduction: Polysaccharides, key components present in Grifola frondosa, can be divided into those derived from fruiting bodies, mycelium, and fermentation broth based on their source. The structure of G. frondosa fruiting body-derived polysaccharides has been fully characterized. However, the structure of G. frondosa mycelium-derived polysaccharides remains to be elucidated. Methods: In this study, we obtained mycelia from G. frondosa by liquid fermentation and extracted them with water and alkaline solution. Then, the mycelia were isolated and purified to obtain homogeneity and systematically characterized by methylation and FT infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy. Results and discussion: Structural analysis showed that two neutral fractions (WGFP-N-a and AGFP-N-a(1)) have a common backbone composed of α-1,6-D-Me-Galp and α-1,6-D-Galp that were substituted at O-2 by 1,2-Manp, α-1,3-L-Fucp, and α-T-D-Manp and thus are identified as fucomannogalactans. WGFP-A-a, AGFP-A-b, and AGFP-A-c are β-1,6-glucans with different molecular weights and are branched with β-1,3-D-Glcp and T-D-Glcp at the O-3 of Glc. Our results provide important structural information about G. frondosa mycelium-derived polysaccharides and provide the basis for their further development and application. Frontiers Media S.A. 2023-08-07 /pmc/articles/PMC10441114/ /pubmed/37608863 http://dx.doi.org/10.3389/fchem.2023.1227288 Text en Copyright © 2023 Geng, Wang, Guo, Han, Qu, Zhou, Tai, Sun and Cheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Geng, Jie Wang, Guining Guo, Jiao Han, Xiao Qu, Yunhe Zhou, Yifa Tai, Guihua Sun, Lin Cheng, Hairong Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
title | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
title_full | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
title_fullStr | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
title_full_unstemmed | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
title_short | Preparation and structural analysis of fucomannogalactan and β-1,6-glucan from Grifola frondosa mycelium |
title_sort | preparation and structural analysis of fucomannogalactan and β-1,6-glucan from grifola frondosa mycelium |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441114/ https://www.ncbi.nlm.nih.gov/pubmed/37608863 http://dx.doi.org/10.3389/fchem.2023.1227288 |
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