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Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring
Understanding the intricate interplay and inter-connectivity of biological processes across an entire organism is important in various fields of biology, including cardiovascular research, neuroscience, and developmental biology. Here, we present a mesoscopic oblique plane microscope (OPM) that enab...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441428/ https://www.ncbi.nlm.nih.gov/pubmed/37609162 http://dx.doi.org/10.1101/2023.08.10.552834 |
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author | DAETWYLER, STEPHAN CHANG, BO-JUI CHEN, BINGYING ZHOU, FELIX FIOLKA, RETO |
author_facet | DAETWYLER, STEPHAN CHANG, BO-JUI CHEN, BINGYING ZHOU, FELIX FIOLKA, RETO |
author_sort | DAETWYLER, STEPHAN |
collection | PubMed |
description | Understanding the intricate interplay and inter-connectivity of biological processes across an entire organism is important in various fields of biology, including cardiovascular research, neuroscience, and developmental biology. Here, we present a mesoscopic oblique plane microscope (OPM) that enables whole organism imaging with high speed and subcellular resolution. A microprism underneath the sample enhances the axial resolution and optical sectioning through total internal reflection of the light-sheet. Through rapid refocusing of the light-sheet, the imaging depth is extended up to threefold while keeping the axial resolution constant. Using low magnification objectives with a large field of view, we realize mesoscopic imaging over a volume of 3.7×1.5×1 mm(3) with ~2.3 microns lateral and ~9.2 microns axial resolution. Applying the mesoscopic OPM, we demonstrate in vivo and in toto whole organism imaging of the zebrafish vasculature and its endothelial nuclei, and blood flow dynamics at 12 Hz acquisition rate, resulting in a quantitative map of blood flow across the entire organism. |
format | Online Article Text |
id | pubmed-10441428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-104414282023-08-22 Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring DAETWYLER, STEPHAN CHANG, BO-JUI CHEN, BINGYING ZHOU, FELIX FIOLKA, RETO bioRxiv Article Understanding the intricate interplay and inter-connectivity of biological processes across an entire organism is important in various fields of biology, including cardiovascular research, neuroscience, and developmental biology. Here, we present a mesoscopic oblique plane microscope (OPM) that enables whole organism imaging with high speed and subcellular resolution. A microprism underneath the sample enhances the axial resolution and optical sectioning through total internal reflection of the light-sheet. Through rapid refocusing of the light-sheet, the imaging depth is extended up to threefold while keeping the axial resolution constant. Using low magnification objectives with a large field of view, we realize mesoscopic imaging over a volume of 3.7×1.5×1 mm(3) with ~2.3 microns lateral and ~9.2 microns axial resolution. Applying the mesoscopic OPM, we demonstrate in vivo and in toto whole organism imaging of the zebrafish vasculature and its endothelial nuclei, and blood flow dynamics at 12 Hz acquisition rate, resulting in a quantitative map of blood flow across the entire organism. Cold Spring Harbor Laboratory 2023-08-11 /pmc/articles/PMC10441428/ /pubmed/37609162 http://dx.doi.org/10.1101/2023.08.10.552834 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article DAETWYLER, STEPHAN CHANG, BO-JUI CHEN, BINGYING ZHOU, FELIX FIOLKA, RETO Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring |
title | Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring |
title_full | Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring |
title_fullStr | Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring |
title_full_unstemmed | Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring |
title_short | Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring |
title_sort | mesoscopic oblique plane microscopy via light-sheet mirroring |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441428/ https://www.ncbi.nlm.nih.gov/pubmed/37609162 http://dx.doi.org/10.1101/2023.08.10.552834 |
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