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VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desir...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441944/ https://www.ncbi.nlm.nih.gov/pubmed/37676518 http://dx.doi.org/10.1007/s44154-021-00026-x |
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author | Gentzel, Irene N. Ohlson, Erik W. Redinbaugh, Margaret G. Wang, Guo-Liang |
author_facet | Gentzel, Irene N. Ohlson, Erik W. Redinbaugh, Margaret G. Wang, Guo-Liang |
author_sort | Gentzel, Irene N. |
collection | PubMed |
description | Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desirable cultivars may not be amenable to genetic transformation, necessitating inferior cultivar usage and time-consuming introgression through backcrossing to the preferred variety. Overcoming these limitations will greatly facilitate the development of disease, insect, and abiotic stress tolerant crops. One such avenue for rapid crop improvement is the development of viral systems to deliver CRISPR/Cas-based genome editing technology to plants to generate targeted beneficial mutations. Viral delivery of genomic editing constructs can theoretically be applied to span the entire host range of the virus utilized, circumventing the challenges associated with traditional transformation and breeding techniques. Here we explore the types of viruses that have been optimized for CRISPR/Cas9 delivery, the phenotypic outcomes achieved in recent studies, and discuss the future potential of this rapidly advancing technology. |
format | Online Article Text |
id | pubmed-10441944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-104419442023-08-28 VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants Gentzel, Irene N. Ohlson, Erik W. Redinbaugh, Margaret G. Wang, Guo-Liang Stress Biol Review Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desirable cultivars may not be amenable to genetic transformation, necessitating inferior cultivar usage and time-consuming introgression through backcrossing to the preferred variety. Overcoming these limitations will greatly facilitate the development of disease, insect, and abiotic stress tolerant crops. One such avenue for rapid crop improvement is the development of viral systems to deliver CRISPR/Cas-based genome editing technology to plants to generate targeted beneficial mutations. Viral delivery of genomic editing constructs can theoretically be applied to span the entire host range of the virus utilized, circumventing the challenges associated with traditional transformation and breeding techniques. Here we explore the types of viruses that have been optimized for CRISPR/Cas9 delivery, the phenotypic outcomes achieved in recent studies, and discuss the future potential of this rapidly advancing technology. Springer Singapore 2022-01-07 /pmc/articles/PMC10441944/ /pubmed/37676518 http://dx.doi.org/10.1007/s44154-021-00026-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Gentzel, Irene N. Ohlson, Erik W. Redinbaugh, Margaret G. Wang, Guo-Liang VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
title | VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
title_full | VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
title_fullStr | VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
title_full_unstemmed | VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
title_short | VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
title_sort | vige: virus-induced genome editing for improving abiotic and biotic stress traits in plants |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441944/ https://www.ncbi.nlm.nih.gov/pubmed/37676518 http://dx.doi.org/10.1007/s44154-021-00026-x |
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