Cargando…

VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants

Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desir...

Descripción completa

Detalles Bibliográficos
Autores principales: Gentzel, Irene N., Ohlson, Erik W., Redinbaugh, Margaret G., Wang, Guo-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441944/
https://www.ncbi.nlm.nih.gov/pubmed/37676518
http://dx.doi.org/10.1007/s44154-021-00026-x
_version_ 1785093481930162176
author Gentzel, Irene N.
Ohlson, Erik W.
Redinbaugh, Margaret G.
Wang, Guo-Liang
author_facet Gentzel, Irene N.
Ohlson, Erik W.
Redinbaugh, Margaret G.
Wang, Guo-Liang
author_sort Gentzel, Irene N.
collection PubMed
description Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desirable cultivars may not be amenable to genetic transformation, necessitating inferior cultivar usage and time-consuming introgression through backcrossing to the preferred variety. Overcoming these limitations will greatly facilitate the development of disease, insect, and abiotic stress tolerant crops. One such avenue for rapid crop improvement is the development of viral systems to deliver CRISPR/Cas-based genome editing technology to plants to generate targeted beneficial mutations. Viral delivery of genomic editing constructs can theoretically be applied to span the entire host range of the virus utilized, circumventing the challenges associated with traditional transformation and breeding techniques. Here we explore the types of viruses that have been optimized for CRISPR/Cas9 delivery, the phenotypic outcomes achieved in recent studies, and discuss the future potential of this rapidly advancing technology.
format Online
Article
Text
id pubmed-10441944
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Singapore
record_format MEDLINE/PubMed
spelling pubmed-104419442023-08-28 VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants Gentzel, Irene N. Ohlson, Erik W. Redinbaugh, Margaret G. Wang, Guo-Liang Stress Biol Review Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desirable cultivars may not be amenable to genetic transformation, necessitating inferior cultivar usage and time-consuming introgression through backcrossing to the preferred variety. Overcoming these limitations will greatly facilitate the development of disease, insect, and abiotic stress tolerant crops. One such avenue for rapid crop improvement is the development of viral systems to deliver CRISPR/Cas-based genome editing technology to plants to generate targeted beneficial mutations. Viral delivery of genomic editing constructs can theoretically be applied to span the entire host range of the virus utilized, circumventing the challenges associated with traditional transformation and breeding techniques. Here we explore the types of viruses that have been optimized for CRISPR/Cas9 delivery, the phenotypic outcomes achieved in recent studies, and discuss the future potential of this rapidly advancing technology. Springer Singapore 2022-01-07 /pmc/articles/PMC10441944/ /pubmed/37676518 http://dx.doi.org/10.1007/s44154-021-00026-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Gentzel, Irene N.
Ohlson, Erik W.
Redinbaugh, Margaret G.
Wang, Guo-Liang
VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
title VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
title_full VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
title_fullStr VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
title_full_unstemmed VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
title_short VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants
title_sort vige: virus-induced genome editing for improving abiotic and biotic stress traits in plants
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441944/
https://www.ncbi.nlm.nih.gov/pubmed/37676518
http://dx.doi.org/10.1007/s44154-021-00026-x
work_keys_str_mv AT gentzelirenen vigevirusinducedgenomeeditingforimprovingabioticandbioticstresstraitsinplants
AT ohlsonerikw vigevirusinducedgenomeeditingforimprovingabioticandbioticstresstraitsinplants
AT redinbaughmargaretg vigevirusinducedgenomeeditingforimprovingabioticandbioticstresstraitsinplants
AT wangguoliang vigevirusinducedgenomeeditingforimprovingabioticandbioticstresstraitsinplants