Cargando…

A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress

Maintenance of root elongation is beneficial for the growth and survival of plants under salt stress, but currently the cellular components involved in the regulation of root growth under high salinity are not fully understood. In this study, we identified an Arabidopsis mutant, rres1, which exhibit...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Zheping, Ren, Yuying, Liu, Jianwei, Zhu, Jian-Kang, Zhao, Chunzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441957/
https://www.ncbi.nlm.nih.gov/pubmed/37676421
http://dx.doi.org/10.1007/s44154-022-00036-3
_version_ 1785093484313575424
author Yu, Zheping
Ren, Yuying
Liu, Jianwei
Zhu, Jian-Kang
Zhao, Chunzhao
author_facet Yu, Zheping
Ren, Yuying
Liu, Jianwei
Zhu, Jian-Kang
Zhao, Chunzhao
author_sort Yu, Zheping
collection PubMed
description Maintenance of root elongation is beneficial for the growth and survival of plants under salt stress, but currently the cellular components involved in the regulation of root growth under high salinity are not fully understood. In this study, we identified an Arabidopsis mutant, rres1, which exhibited reduced root elongation under treatment of a variety of salts, including NaCl, NaNO(3), KCl, and KNO(3). RRES1 encodes a novel mitochondrial protein and its molecular function is still unknown. Under salt stress, the root meristem length was shorter in the rres1 mutant compared to the wild type, which was correlated with a reduced auxin accumulation in the mutant. Reactive oxygen species (ROS), as important signals that regulate root elongation, were accumulated to higher levels in the rres1 mutant than the wild type after salt treatment. Measurement of monosaccharides in the cell wall showed that arabinose and xylose contents were decreased in the rres1 mutant under salt stress, and application of boric acid, which is required for the crosslinking of pectic polysaccharide rhamnogalacturonan-II (RG-II), largely rescued the root growth arrest of the rres1 mutant, suggesting that RRES1 participates in the maintenance of cell wall integrity under salt stress. GUS staining assay indicated that the RRES1 gene was expressed in leaves and weakly in root tip under normal conditions, but its expression was dramatically increased in leaves and roots after salt treatment. Together, our study reveals a novel mitochondrial protein that regulates root elongation under salt stress via the modulation of cell wall integrity, auxin accumulation, and ROS homeostasis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s44154-022-00036-3.
format Online
Article
Text
id pubmed-10441957
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Nature Singapore
record_format MEDLINE/PubMed
spelling pubmed-104419572023-08-28 A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress Yu, Zheping Ren, Yuying Liu, Jianwei Zhu, Jian-Kang Zhao, Chunzhao Stress Biol Original Paper Maintenance of root elongation is beneficial for the growth and survival of plants under salt stress, but currently the cellular components involved in the regulation of root growth under high salinity are not fully understood. In this study, we identified an Arabidopsis mutant, rres1, which exhibited reduced root elongation under treatment of a variety of salts, including NaCl, NaNO(3), KCl, and KNO(3). RRES1 encodes a novel mitochondrial protein and its molecular function is still unknown. Under salt stress, the root meristem length was shorter in the rres1 mutant compared to the wild type, which was correlated with a reduced auxin accumulation in the mutant. Reactive oxygen species (ROS), as important signals that regulate root elongation, were accumulated to higher levels in the rres1 mutant than the wild type after salt treatment. Measurement of monosaccharides in the cell wall showed that arabinose and xylose contents were decreased in the rres1 mutant under salt stress, and application of boric acid, which is required for the crosslinking of pectic polysaccharide rhamnogalacturonan-II (RG-II), largely rescued the root growth arrest of the rres1 mutant, suggesting that RRES1 participates in the maintenance of cell wall integrity under salt stress. GUS staining assay indicated that the RRES1 gene was expressed in leaves and weakly in root tip under normal conditions, but its expression was dramatically increased in leaves and roots after salt treatment. Together, our study reveals a novel mitochondrial protein that regulates root elongation under salt stress via the modulation of cell wall integrity, auxin accumulation, and ROS homeostasis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s44154-022-00036-3. Springer Nature Singapore 2022-02-08 /pmc/articles/PMC10441957/ /pubmed/37676421 http://dx.doi.org/10.1007/s44154-022-00036-3 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Yu, Zheping
Ren, Yuying
Liu, Jianwei
Zhu, Jian-Kang
Zhao, Chunzhao
A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress
title A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress
title_full A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress
title_fullStr A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress
title_full_unstemmed A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress
title_short A novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in Arabidopsis under salt stress
title_sort novel mitochondrial protein is required for cell wall integrity, auxin accumulation and root elongation in arabidopsis under salt stress
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10441957/
https://www.ncbi.nlm.nih.gov/pubmed/37676421
http://dx.doi.org/10.1007/s44154-022-00036-3
work_keys_str_mv AT yuzheping anovelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT renyuying anovelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT liujianwei anovelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT zhujiankang anovelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT zhaochunzhao anovelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT yuzheping novelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT renyuying novelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT liujianwei novelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT zhujiankang novelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress
AT zhaochunzhao novelmitochondrialproteinisrequiredforcellwallintegrityauxinaccumulationandrootelongationinarabidopsisundersaltstress