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Root-knot nematode assessment: species identification, distribution, and new host records in Portugal

Considered one of the most devastating plant parasitic nematodes worldwide, Meloidogyne spp. (commonly known as the root-knot nematodes (RKNs)) are obligate sedentary endoparasites that establish in the roots, causing hyperplasia and hypertrophy of surrounding cells, triggering the formation of gall...

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Autores principales: Rusinque, Leidy, Camacho, Maria João, Serra, Clara, Nóbrega, Filomena, Inácio, Maria L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10442539/
https://www.ncbi.nlm.nih.gov/pubmed/37615023
http://dx.doi.org/10.3389/fpls.2023.1230968
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author Rusinque, Leidy
Camacho, Maria João
Serra, Clara
Nóbrega, Filomena
Inácio, Maria L.
author_facet Rusinque, Leidy
Camacho, Maria João
Serra, Clara
Nóbrega, Filomena
Inácio, Maria L.
author_sort Rusinque, Leidy
collection PubMed
description Considered one of the most devastating plant parasitic nematodes worldwide, Meloidogyne spp. (commonly known as the root-knot nematodes (RKNs)) are obligate sedentary endoparasites that establish in the roots, causing hyperplasia and hypertrophy of surrounding cells, triggering the formation of galls. These galls will affect root development and physiology, leading to substantial yield losses. During 2017–2022, an extensive survey of Meloidogyne species was undertaken in Portugal (mainland and islands). A total of 1,071 samples were collected by the National Plant Protection Organization (DGAV) and private farmers from different regions of the country and were analysed at the Laboratory of Nematology (NemaINIAV). Samples in which the presence of Meloidogyne sp. was detected were used to perform bioassays to obtain females and juveniles for further studies. Since the accurate identification of RKNs is an important aspect of crop management, morphological and biochemical characterisation was performed. The most common morphological features were observed, showing consistency with previous descriptions of the genus. The biochemical identification using the esterase (EST) phenotype revealed the phenotypes of Meloidogyne arenaria, M enterolobi, M. hispanica, M. hapla, M. incognita, M javanica, and M. luci. Meloidogyne incognita and M. javanica were found to be the most prevalent species in the different regions followed by M. arenaria and M. hapla. This is the first distribution report performed in Portugal on RKNs, contributing to the development of management strategies and to updated information on the status of these pests in Europe.
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spelling pubmed-104425392023-08-23 Root-knot nematode assessment: species identification, distribution, and new host records in Portugal Rusinque, Leidy Camacho, Maria João Serra, Clara Nóbrega, Filomena Inácio, Maria L. Front Plant Sci Plant Science Considered one of the most devastating plant parasitic nematodes worldwide, Meloidogyne spp. (commonly known as the root-knot nematodes (RKNs)) are obligate sedentary endoparasites that establish in the roots, causing hyperplasia and hypertrophy of surrounding cells, triggering the formation of galls. These galls will affect root development and physiology, leading to substantial yield losses. During 2017–2022, an extensive survey of Meloidogyne species was undertaken in Portugal (mainland and islands). A total of 1,071 samples were collected by the National Plant Protection Organization (DGAV) and private farmers from different regions of the country and were analysed at the Laboratory of Nematology (NemaINIAV). Samples in which the presence of Meloidogyne sp. was detected were used to perform bioassays to obtain females and juveniles for further studies. Since the accurate identification of RKNs is an important aspect of crop management, morphological and biochemical characterisation was performed. The most common morphological features were observed, showing consistency with previous descriptions of the genus. The biochemical identification using the esterase (EST) phenotype revealed the phenotypes of Meloidogyne arenaria, M enterolobi, M. hispanica, M. hapla, M. incognita, M javanica, and M. luci. Meloidogyne incognita and M. javanica were found to be the most prevalent species in the different regions followed by M. arenaria and M. hapla. This is the first distribution report performed in Portugal on RKNs, contributing to the development of management strategies and to updated information on the status of these pests in Europe. Frontiers Media S.A. 2023-08-08 /pmc/articles/PMC10442539/ /pubmed/37615023 http://dx.doi.org/10.3389/fpls.2023.1230968 Text en Copyright © 2023 Rusinque, Camacho, Serra, Nóbrega and Inácio https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Rusinque, Leidy
Camacho, Maria João
Serra, Clara
Nóbrega, Filomena
Inácio, Maria L.
Root-knot nematode assessment: species identification, distribution, and new host records in Portugal
title Root-knot nematode assessment: species identification, distribution, and new host records in Portugal
title_full Root-knot nematode assessment: species identification, distribution, and new host records in Portugal
title_fullStr Root-knot nematode assessment: species identification, distribution, and new host records in Portugal
title_full_unstemmed Root-knot nematode assessment: species identification, distribution, and new host records in Portugal
title_short Root-knot nematode assessment: species identification, distribution, and new host records in Portugal
title_sort root-knot nematode assessment: species identification, distribution, and new host records in portugal
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10442539/
https://www.ncbi.nlm.nih.gov/pubmed/37615023
http://dx.doi.org/10.3389/fpls.2023.1230968
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