Cargando…

In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines

Treatment with extracts from whole herbs has been reported to synergistically enhance the anticancer activities of therapeutic agents in recent studies. The present study evaluated the antioxidant and anticancer activities of Smilax corbularia Kunth (S. corbularia) and Phellinus linteus (P. linteus)...

Descripción completa

Detalles Bibliográficos
Autores principales: Syukriya, Alvi Jauharotus, Bankeeree, Wichanee, Prasongsuk, Sehanat, Yanatatsaneejit, Pattamawadee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10442767/
https://www.ncbi.nlm.nih.gov/pubmed/37614981
http://dx.doi.org/10.3892/br.2023.1645
_version_ 1785093674619633664
author Syukriya, Alvi Jauharotus
Bankeeree, Wichanee
Prasongsuk, Sehanat
Yanatatsaneejit, Pattamawadee
author_facet Syukriya, Alvi Jauharotus
Bankeeree, Wichanee
Prasongsuk, Sehanat
Yanatatsaneejit, Pattamawadee
author_sort Syukriya, Alvi Jauharotus
collection PubMed
description Treatment with extracts from whole herbs has been reported to synergistically enhance the anticancer activities of therapeutic agents in recent studies. The present study evaluated the antioxidant and anticancer activities of Smilax corbularia Kunth (S. corbularia) and Phellinus linteus (P. linteus) crude extracts individually and in combination. S. corbularia was extracted using ethanol, whereas P. linteus was extracted using hot water. Both crude extracts underwent physiochemical characterization. Subsequently, the possible antioxidant activities of both crude extracts, individually and in combination, were evaluated using 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays. Their effects on breast cancer cell cytotoxicity, proliferation and apoptosis were then assessed. The crude S. corbularia extract obtained was found to have a high level of total phenolic content, whilst the crude P. linteus extract had high levels of total polysaccharide content. The total phenolic content and total polysaccharide content results of the combinations depended on the respective ratios of the individual extracts. S. corbularia alone and combination 3 (which contained 75% S. corbularia: 25% P. linteus) demonstrated the greatest radical scavenging activity, followed by combination 1 (50% S. corbularia: 50% P. linteus), combination 2 (25% S. corbularia: 75% P. linteus) and P. linteus. The toxicity results of the extract samples on the cancer cells corresponded with their antioxidant activity. In particular, certain combinations demonstrated clearer inhibitory effects on cell proliferation against three types of breast cancer cells compared with those exerted by the two individual extracts. However, induction of apoptosis was limited, with the degree of apoptosis observed to be #x003C;5%. These findings suggested that treatment with combinations of these two extracts could confer enhanced antioxidant and antiproliferative effects on breast cancer cells. Therefore, the potential of these two extracts in combination as anticancer agents warrants further investigation.
format Online
Article
Text
id pubmed-10442767
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-104427672023-08-23 In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines Syukriya, Alvi Jauharotus Bankeeree, Wichanee Prasongsuk, Sehanat Yanatatsaneejit, Pattamawadee Biomed Rep Articles Treatment with extracts from whole herbs has been reported to synergistically enhance the anticancer activities of therapeutic agents in recent studies. The present study evaluated the antioxidant and anticancer activities of Smilax corbularia Kunth (S. corbularia) and Phellinus linteus (P. linteus) crude extracts individually and in combination. S. corbularia was extracted using ethanol, whereas P. linteus was extracted using hot water. Both crude extracts underwent physiochemical characterization. Subsequently, the possible antioxidant activities of both crude extracts, individually and in combination, were evaluated using 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays. Their effects on breast cancer cell cytotoxicity, proliferation and apoptosis were then assessed. The crude S. corbularia extract obtained was found to have a high level of total phenolic content, whilst the crude P. linteus extract had high levels of total polysaccharide content. The total phenolic content and total polysaccharide content results of the combinations depended on the respective ratios of the individual extracts. S. corbularia alone and combination 3 (which contained 75% S. corbularia: 25% P. linteus) demonstrated the greatest radical scavenging activity, followed by combination 1 (50% S. corbularia: 50% P. linteus), combination 2 (25% S. corbularia: 75% P. linteus) and P. linteus. The toxicity results of the extract samples on the cancer cells corresponded with their antioxidant activity. In particular, certain combinations demonstrated clearer inhibitory effects on cell proliferation against three types of breast cancer cells compared with those exerted by the two individual extracts. However, induction of apoptosis was limited, with the degree of apoptosis observed to be #x003C;5%. These findings suggested that treatment with combinations of these two extracts could confer enhanced antioxidant and antiproliferative effects on breast cancer cells. Therefore, the potential of these two extracts in combination as anticancer agents warrants further investigation. D.A. Spandidos 2023-08-07 /pmc/articles/PMC10442767/ /pubmed/37614981 http://dx.doi.org/10.3892/br.2023.1645 Text en Copyright: © Syukriya et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Syukriya, Alvi Jauharotus
Bankeeree, Wichanee
Prasongsuk, Sehanat
Yanatatsaneejit, Pattamawadee
In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines
title In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines
title_full In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines
title_fullStr In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines
title_full_unstemmed In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines
title_short In vitro antioxidant and anticancer activities of Smilax corbularia extract combined with Phellinus linteus extract against breast cancer cell lines
title_sort in vitro antioxidant and anticancer activities of smilax corbularia extract combined with phellinus linteus extract against breast cancer cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10442767/
https://www.ncbi.nlm.nih.gov/pubmed/37614981
http://dx.doi.org/10.3892/br.2023.1645
work_keys_str_mv AT syukriyaalvijauharotus invitroantioxidantandanticanceractivitiesofsmilaxcorbulariaextractcombinedwithphellinuslinteusextractagainstbreastcancercelllines
AT bankeereewichanee invitroantioxidantandanticanceractivitiesofsmilaxcorbulariaextractcombinedwithphellinuslinteusextractagainstbreastcancercelllines
AT prasongsuksehanat invitroantioxidantandanticanceractivitiesofsmilaxcorbulariaextractcombinedwithphellinuslinteusextractagainstbreastcancercelllines
AT yanatatsaneejitpattamawadee invitroantioxidantandanticanceractivitiesofsmilaxcorbulariaextractcombinedwithphellinuslinteusextractagainstbreastcancercelllines