Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in Saccharomyces cerevisiae

[Image: see text] Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology, with its ability to target a specific DNA locus using guide RNAs (gRNAs), is particularly suited for targeted mutagenesis. The targeted diversification of nucleotides in Saccharomyces cerevisiae usi...

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Detalles Bibliográficos
Autores principales: Gossing, Michael, Limeta, Angelo, Skrekas, Christos, Wigglesworth, Mark, Davis, Andrew, Siewers, Verena, David, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443033/
https://www.ncbi.nlm.nih.gov/pubmed/37486342
http://dx.doi.org/10.1021/acssynbio.2c00689

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