SARS-CoV-2 Virus-like Particles with Plasmonic Au Cores and S1-Spike Protein Coronas

[Image: see text] The COVID-19 pandemic has stimulated the scientific world to intensify virus-related studies aimed at the development of quick and safe ways of detecting viruses in the human body, studying the virus–antibody and virus–cell interactions, and designing nanocarriers for targeted antiviral therapies. However, research on dangerous viruses can only be performed in certified laboratories that follow strict safety procedures. Thus, developing deactivated virus constructs or safe-to-use virus-like objects, which imitate real viruses and allow performing virus-related studies in any research laboratory, constitutes an important scientific challenge. Such species, called virus-like particles (VLPs), contain instead of capsids with viral DNA/RNA empty or synthetic cores with real virus proteins attached to them. We have developed a method for the preparation of VLPs imitating the virus responsible for the COVID-19 disease: the SARS-CoV-2. The particles have Au cores surrounded by “coronas” of S1 domains of the virus’s spike protein. Importantly, they are safe to use and specifically interact with SARS-CoV-2 antibodies. Moreover, Au cores exhibit localized surface plasmon resonance (LSPR), which makes the synthesized VLPs suitable for biosensing applications. During the studies, the effect allowed us to visualize the interaction between the VLPs and the antibodies and identify the characteristic vibrational signals. What is more, additional functionalization of the particles with a fluorescent label revealed their potential in studying specific virus-related interactions. Notably, the universal character of the developed synthesis method makes it potentially applicable for fabricating VLPs imitating other life-threatening viruses.