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Biofilm formation on the surface of monazite and xenotime during bioleaching

Microbial attachment and biofilm formation is a ubiquitous behaviour of microorganisms and is the most crucial prerequisite of contact bioleaching. Monazite and xenotime are two commercially exploitable minerals containing rare earth elements (REEs). Bioleaching using phosphate solubilizing microorg...

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Autores principales: van Alin, Arya, Corbett, Melissa K., Fathollahzadeh, Homayoun, Tjiam, M. Christian, Rickard, William D. A., Sun, Xiao, Putnis, Andrew, Eksteen, Jacques, Kaksonen, Anna H., Watkin, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443343/
https://www.ncbi.nlm.nih.gov/pubmed/37291762
http://dx.doi.org/10.1111/1751-7915.14260
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author van Alin, Arya
Corbett, Melissa K.
Fathollahzadeh, Homayoun
Tjiam, M. Christian
Rickard, William D. A.
Sun, Xiao
Putnis, Andrew
Eksteen, Jacques
Kaksonen, Anna H.
Watkin, Elizabeth
author_facet van Alin, Arya
Corbett, Melissa K.
Fathollahzadeh, Homayoun
Tjiam, M. Christian
Rickard, William D. A.
Sun, Xiao
Putnis, Andrew
Eksteen, Jacques
Kaksonen, Anna H.
Watkin, Elizabeth
author_sort van Alin, Arya
collection PubMed
description Microbial attachment and biofilm formation is a ubiquitous behaviour of microorganisms and is the most crucial prerequisite of contact bioleaching. Monazite and xenotime are two commercially exploitable minerals containing rare earth elements (REEs). Bioleaching using phosphate solubilizing microorganisms is a green biotechnological approach for the extraction of REEs. In this study, microbial attachment and biofilm formation of Klebsiella aerogenes ATCC 13048 on the surface of these minerals were investigated using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). In a batch culture system, K. aerogenes was able to attach and form biofilms on the surface of three phosphate minerals. The microscopy records showed three distinctive stages of biofilm development for K. aerogenes commencing with initial attachment to the surface occurring in the first minutes of microbial inoculation. This was followed by colonization of the surface and formation of a mature biofilm as the second distinguishable stage, with progression to dispersion as the final stage. The biofilm had a thin‐layer structure. The colonization and biofilm formation were localized toward physical surface imperfections such as cracks, pits, grooves and dents. In comparison to monazite and xenotime crystals, a higher proportion of the surface of the high‐grade monazite ore was covered by biofilm which could be due to its higher surface roughness. No selective attachment or colonization toward specific mineralogy or chemical composition of the minerals was detected. Finally, in contrast to abiotic leaching of control samples, microbial activity resulted in extensive microbial erosion on the high‐grade monazite ore.
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spelling pubmed-104433432023-08-23 Biofilm formation on the surface of monazite and xenotime during bioleaching van Alin, Arya Corbett, Melissa K. Fathollahzadeh, Homayoun Tjiam, M. Christian Rickard, William D. A. Sun, Xiao Putnis, Andrew Eksteen, Jacques Kaksonen, Anna H. Watkin, Elizabeth Microb Biotechnol Research Articles Microbial attachment and biofilm formation is a ubiquitous behaviour of microorganisms and is the most crucial prerequisite of contact bioleaching. Monazite and xenotime are two commercially exploitable minerals containing rare earth elements (REEs). Bioleaching using phosphate solubilizing microorganisms is a green biotechnological approach for the extraction of REEs. In this study, microbial attachment and biofilm formation of Klebsiella aerogenes ATCC 13048 on the surface of these minerals were investigated using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). In a batch culture system, K. aerogenes was able to attach and form biofilms on the surface of three phosphate minerals. The microscopy records showed three distinctive stages of biofilm development for K. aerogenes commencing with initial attachment to the surface occurring in the first minutes of microbial inoculation. This was followed by colonization of the surface and formation of a mature biofilm as the second distinguishable stage, with progression to dispersion as the final stage. The biofilm had a thin‐layer structure. The colonization and biofilm formation were localized toward physical surface imperfections such as cracks, pits, grooves and dents. In comparison to monazite and xenotime crystals, a higher proportion of the surface of the high‐grade monazite ore was covered by biofilm which could be due to its higher surface roughness. No selective attachment or colonization toward specific mineralogy or chemical composition of the minerals was detected. Finally, in contrast to abiotic leaching of control samples, microbial activity resulted in extensive microbial erosion on the high‐grade monazite ore. John Wiley and Sons Inc. 2023-06-08 /pmc/articles/PMC10443343/ /pubmed/37291762 http://dx.doi.org/10.1111/1751-7915.14260 Text en © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
van Alin, Arya
Corbett, Melissa K.
Fathollahzadeh, Homayoun
Tjiam, M. Christian
Rickard, William D. A.
Sun, Xiao
Putnis, Andrew
Eksteen, Jacques
Kaksonen, Anna H.
Watkin, Elizabeth
Biofilm formation on the surface of monazite and xenotime during bioleaching
title Biofilm formation on the surface of monazite and xenotime during bioleaching
title_full Biofilm formation on the surface of monazite and xenotime during bioleaching
title_fullStr Biofilm formation on the surface of monazite and xenotime during bioleaching
title_full_unstemmed Biofilm formation on the surface of monazite and xenotime during bioleaching
title_short Biofilm formation on the surface of monazite and xenotime during bioleaching
title_sort biofilm formation on the surface of monazite and xenotime during bioleaching
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443343/
https://www.ncbi.nlm.nih.gov/pubmed/37291762
http://dx.doi.org/10.1111/1751-7915.14260
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