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Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443378/ https://www.ncbi.nlm.nih.gov/pubmed/37606419 http://dx.doi.org/10.3390/proteomes11030023 |
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author | Jimenez, Dianny Elizabeth Tahir, Muhammad Faheem, Muhammad Alves, Wellington Bruno dos Santos Correa, Barbara de Lucena de Andrade, Gabriel Rocha Larsen, Martin R. de Oliveira, Getulio Pereira Pereira, Rinaldo Wellerson |
author_facet | Jimenez, Dianny Elizabeth Tahir, Muhammad Faheem, Muhammad Alves, Wellington Bruno dos Santos Correa, Barbara de Lucena de Andrade, Gabriel Rocha Larsen, Martin R. de Oliveira, Getulio Pereira Pereira, Rinaldo Wellerson |
author_sort | Jimenez, Dianny Elizabeth |
collection | PubMed |
description | In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are also moldable tools for the controlled release of bioactive substances in clinical and therapeutic applications. However, one of the significant challenges when studying these exciting and versatile vesicles is the purification process, which presents significant difficulties in terms of lack of purity, yield, and reproducibility, reflected in unreliable data. Therefore, our objective in the present study was to compare the proteomic profile of serum-derived EVs purified using ExoQuick™ (Systems Biosciences), Total Isolation Kit (Life Technologies), Ultracentrifugation, and Ultrafiltration. Each technique utilized for purification has shown different concentrations and populations of purified particles. The results showed marked differences in distribution, size, and protein content, demonstrating the need to develop reproducible and reliable protocols to isolate extracellular vesicles for their clinical application. |
format | Online Article Text |
id | pubmed-10443378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104433782023-08-23 Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics Jimenez, Dianny Elizabeth Tahir, Muhammad Faheem, Muhammad Alves, Wellington Bruno dos Santos Correa, Barbara de Lucena de Andrade, Gabriel Rocha Larsen, Martin R. de Oliveira, Getulio Pereira Pereira, Rinaldo Wellerson Proteomes Article In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are also moldable tools for the controlled release of bioactive substances in clinical and therapeutic applications. However, one of the significant challenges when studying these exciting and versatile vesicles is the purification process, which presents significant difficulties in terms of lack of purity, yield, and reproducibility, reflected in unreliable data. Therefore, our objective in the present study was to compare the proteomic profile of serum-derived EVs purified using ExoQuick™ (Systems Biosciences), Total Isolation Kit (Life Technologies), Ultracentrifugation, and Ultrafiltration. Each technique utilized for purification has shown different concentrations and populations of purified particles. The results showed marked differences in distribution, size, and protein content, demonstrating the need to develop reproducible and reliable protocols to isolate extracellular vesicles for their clinical application. MDPI 2023-07-25 /pmc/articles/PMC10443378/ /pubmed/37606419 http://dx.doi.org/10.3390/proteomes11030023 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jimenez, Dianny Elizabeth Tahir, Muhammad Faheem, Muhammad Alves, Wellington Bruno dos Santos Correa, Barbara de Lucena de Andrade, Gabriel Rocha Larsen, Martin R. de Oliveira, Getulio Pereira Pereira, Rinaldo Wellerson Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics |
title | Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics |
title_full | Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics |
title_fullStr | Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics |
title_full_unstemmed | Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics |
title_short | Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics |
title_sort | comparison of four purification methods on serum extracellular vesicle recovery, size distribution, and proteomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443378/ https://www.ncbi.nlm.nih.gov/pubmed/37606419 http://dx.doi.org/10.3390/proteomes11030023 |
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