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Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics

In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are...

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Autores principales: Jimenez, Dianny Elizabeth, Tahir, Muhammad, Faheem, Muhammad, Alves, Wellington Bruno dos Santos, Correa, Barbara de Lucena, de Andrade, Gabriel Rocha, Larsen, Martin R., de Oliveira, Getulio Pereira, Pereira, Rinaldo Wellerson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443378/
https://www.ncbi.nlm.nih.gov/pubmed/37606419
http://dx.doi.org/10.3390/proteomes11030023
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author Jimenez, Dianny Elizabeth
Tahir, Muhammad
Faheem, Muhammad
Alves, Wellington Bruno dos Santos
Correa, Barbara de Lucena
de Andrade, Gabriel Rocha
Larsen, Martin R.
de Oliveira, Getulio Pereira
Pereira, Rinaldo Wellerson
author_facet Jimenez, Dianny Elizabeth
Tahir, Muhammad
Faheem, Muhammad
Alves, Wellington Bruno dos Santos
Correa, Barbara de Lucena
de Andrade, Gabriel Rocha
Larsen, Martin R.
de Oliveira, Getulio Pereira
Pereira, Rinaldo Wellerson
author_sort Jimenez, Dianny Elizabeth
collection PubMed
description In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are also moldable tools for the controlled release of bioactive substances in clinical and therapeutic applications. However, one of the significant challenges when studying these exciting and versatile vesicles is the purification process, which presents significant difficulties in terms of lack of purity, yield, and reproducibility, reflected in unreliable data. Therefore, our objective in the present study was to compare the proteomic profile of serum-derived EVs purified using ExoQuick™ (Systems Biosciences), Total Isolation Kit (Life Technologies), Ultracentrifugation, and Ultrafiltration. Each technique utilized for purification has shown different concentrations and populations of purified particles. The results showed marked differences in distribution, size, and protein content, demonstrating the need to develop reproducible and reliable protocols to isolate extracellular vesicles for their clinical application.
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spelling pubmed-104433782023-08-23 Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics Jimenez, Dianny Elizabeth Tahir, Muhammad Faheem, Muhammad Alves, Wellington Bruno dos Santos Correa, Barbara de Lucena de Andrade, Gabriel Rocha Larsen, Martin R. de Oliveira, Getulio Pereira Pereira, Rinaldo Wellerson Proteomes Article In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are also moldable tools for the controlled release of bioactive substances in clinical and therapeutic applications. However, one of the significant challenges when studying these exciting and versatile vesicles is the purification process, which presents significant difficulties in terms of lack of purity, yield, and reproducibility, reflected in unreliable data. Therefore, our objective in the present study was to compare the proteomic profile of serum-derived EVs purified using ExoQuick™ (Systems Biosciences), Total Isolation Kit (Life Technologies), Ultracentrifugation, and Ultrafiltration. Each technique utilized for purification has shown different concentrations and populations of purified particles. The results showed marked differences in distribution, size, and protein content, demonstrating the need to develop reproducible and reliable protocols to isolate extracellular vesicles for their clinical application. MDPI 2023-07-25 /pmc/articles/PMC10443378/ /pubmed/37606419 http://dx.doi.org/10.3390/proteomes11030023 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jimenez, Dianny Elizabeth
Tahir, Muhammad
Faheem, Muhammad
Alves, Wellington Bruno dos Santos
Correa, Barbara de Lucena
de Andrade, Gabriel Rocha
Larsen, Martin R.
de Oliveira, Getulio Pereira
Pereira, Rinaldo Wellerson
Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
title Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
title_full Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
title_fullStr Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
title_full_unstemmed Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
title_short Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics
title_sort comparison of four purification methods on serum extracellular vesicle recovery, size distribution, and proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443378/
https://www.ncbi.nlm.nih.gov/pubmed/37606419
http://dx.doi.org/10.3390/proteomes11030023
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