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Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study

BACKGROUND: Preeclampsia is a pregnancy complication Aim of this study was to investigate expression of Beclin1 and tumor necrosis factor (TNF)-α in normotensive and preeclamptic placentas of pregnant women patients. METHODS: Twenty normotensive and 20 preeclamptic patients placentas were dissected...

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Autores principales: Öcal, Ece, Toprak, Veysel, Akalin, Senem Alkan, Aşir, Firat, Deveci, Engin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443776/
https://www.ncbi.nlm.nih.gov/pubmed/37603530
http://dx.doi.org/10.1097/MD.0000000000034757
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author Öcal, Ece
Toprak, Veysel
Akalin, Senem Alkan
Aşir, Firat
Deveci, Engin
author_facet Öcal, Ece
Toprak, Veysel
Akalin, Senem Alkan
Aşir, Firat
Deveci, Engin
author_sort Öcal, Ece
collection PubMed
description BACKGROUND: Preeclampsia is a pregnancy complication Aim of this study was to investigate expression of Beclin1 and tumor necrosis factor (TNF)-α in normotensive and preeclamptic placentas of pregnant women patients. METHODS: Twenty normotensive and 20 preeclamptic patients placentas were dissected for paraffin- wax processing. Placental samples were embedded in parafin blocks. Sections were stained with Hematoxylin-Eosin staining and TNF-α and Beclin1 immunostaining. RESULTS: In control group, root and floating villi were normal in histological perspectives, syncytial node number was low, vessels were normal with connective tissue. No hemorrhage was observed in the intervillous area. In preeclampsia group, decidual cell degeneration and fibrinoid accumulation increased. Vascular dilatation and congestion with mononuclear cell infiltration were observed. Beclin1 reaction was generally negative in control group. In preeclampsia group, Beclin1 reaction was increased in decidual cells, syncytial nodes and bridges and in chorionic villi and in some Hoffbauer cells. In control group, TNF-α expression was mainly negative but only in some decidual cells. In preeclampsia, TNF-α reaction was observed in degenerated decidua cells, in leukocytes and in villi. CONCLUSION: In preeclampsia placentas, degenerated decidua cells and inflammation increased. It was thought that Beclin1 and TNF-α signals could be used as a marker in affecting the fetal structure of blood flow in preeclamptic placentas.
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spelling pubmed-104437762023-08-23 Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study Öcal, Ece Toprak, Veysel Akalin, Senem Alkan Aşir, Firat Deveci, Engin Medicine (Baltimore) 5600 BACKGROUND: Preeclampsia is a pregnancy complication Aim of this study was to investigate expression of Beclin1 and tumor necrosis factor (TNF)-α in normotensive and preeclamptic placentas of pregnant women patients. METHODS: Twenty normotensive and 20 preeclamptic patients placentas were dissected for paraffin- wax processing. Placental samples were embedded in parafin blocks. Sections were stained with Hematoxylin-Eosin staining and TNF-α and Beclin1 immunostaining. RESULTS: In control group, root and floating villi were normal in histological perspectives, syncytial node number was low, vessels were normal with connective tissue. No hemorrhage was observed in the intervillous area. In preeclampsia group, decidual cell degeneration and fibrinoid accumulation increased. Vascular dilatation and congestion with mononuclear cell infiltration were observed. Beclin1 reaction was generally negative in control group. In preeclampsia group, Beclin1 reaction was increased in decidual cells, syncytial nodes and bridges and in chorionic villi and in some Hoffbauer cells. In control group, TNF-α expression was mainly negative but only in some decidual cells. In preeclampsia, TNF-α reaction was observed in degenerated decidua cells, in leukocytes and in villi. CONCLUSION: In preeclampsia placentas, degenerated decidua cells and inflammation increased. It was thought that Beclin1 and TNF-α signals could be used as a marker in affecting the fetal structure of blood flow in preeclamptic placentas. Lippincott Williams & Wilkins 2023-08-18 /pmc/articles/PMC10443776/ /pubmed/37603530 http://dx.doi.org/10.1097/MD.0000000000034757 Text en Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle 5600
Öcal, Ece
Toprak, Veysel
Akalin, Senem Alkan
Aşir, Firat
Deveci, Engin
Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study
title Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study
title_full Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study
title_fullStr Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study
title_full_unstemmed Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study
title_short Investigation of Beclin 1 and TNF-α expressions in preeclampsia placentas: Immunohistochemical study
title_sort investigation of beclin 1 and tnf-α expressions in preeclampsia placentas: immunohistochemical study
topic 5600
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443776/
https://www.ncbi.nlm.nih.gov/pubmed/37603530
http://dx.doi.org/10.1097/MD.0000000000034757
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