Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay

We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had...

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Detalles Bibliográficos
Autores principales: Slott, Sofie, Krüger-Jensen, Cecilie Schiøth, Ferreira da Silva, Izabela, Pedersen, Nadia Bom, Astakhova, Kira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443835/
https://www.ncbi.nlm.nih.gov/pubmed/37607185
http://dx.doi.org/10.1371/journal.pone.0289556
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author Slott, Sofie
Krüger-Jensen, Cecilie Schiøth
Ferreira da Silva, Izabela
Pedersen, Nadia Bom
Astakhova, Kira
author_facet Slott, Sofie
Krüger-Jensen, Cecilie Schiøth
Ferreira da Silva, Izabela
Pedersen, Nadia Bom
Astakhova, Kira
author_sort Slott, Sofie
collection PubMed
description We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.
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spelling pubmed-104438352023-08-23 Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay Slott, Sofie Krüger-Jensen, Cecilie Schiøth Ferreira da Silva, Izabela Pedersen, Nadia Bom Astakhova, Kira PLoS One Research Article We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer. Public Library of Science 2023-08-22 /pmc/articles/PMC10443835/ /pubmed/37607185 http://dx.doi.org/10.1371/journal.pone.0289556 Text en © 2023 Slott et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Slott, Sofie
Krüger-Jensen, Cecilie Schiøth
Ferreira da Silva, Izabela
Pedersen, Nadia Bom
Astakhova, Kira
Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
title Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
title_full Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
title_fullStr Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
title_full_unstemmed Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
title_short Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
title_sort mutations in microrna-128-2-3p identified with amplification-free hybridization assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443835/
https://www.ncbi.nlm.nih.gov/pubmed/37607185
http://dx.doi.org/10.1371/journal.pone.0289556
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