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Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats

Paracetamol (PAR) is a drug widely used in human and veterinary medicine as an analgesic and antipyretic, often involved in cases of intoxication. The most common clinical signs result from damage to red blood cells and hepatocytes, and this intoxication is considered a model for the induction of ac...

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Autores principales: da Silva, Rayanne Henrique Santana, de Moura, Mariana, de Paula, Larissa, Arantes, Kelly Carolina, da Silva, Marina, de Amorim, Jaqueline, Miguel, Marina Pacheco, Martins, Danieli Brolo, de Melo e Silva, Daniela, Melo, Marília Martins, Botelho, Ana Flávia Machado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443853/
https://www.ncbi.nlm.nih.gov/pubmed/37607187
http://dx.doi.org/10.1371/journal.pone.0290268
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author da Silva, Rayanne Henrique Santana
de Moura, Mariana
de Paula, Larissa
Arantes, Kelly Carolina
da Silva, Marina
de Amorim, Jaqueline
Miguel, Marina Pacheco
Martins, Danieli Brolo
de Melo e Silva, Daniela
Melo, Marília Martins
Botelho, Ana Flávia Machado
author_facet da Silva, Rayanne Henrique Santana
de Moura, Mariana
de Paula, Larissa
Arantes, Kelly Carolina
da Silva, Marina
de Amorim, Jaqueline
Miguel, Marina Pacheco
Martins, Danieli Brolo
de Melo e Silva, Daniela
Melo, Marília Martins
Botelho, Ana Flávia Machado
author_sort da Silva, Rayanne Henrique Santana
collection PubMed
description Paracetamol (PAR) is a drug widely used in human and veterinary medicine as an analgesic and antipyretic, often involved in cases of intoxication. The most common clinical signs result from damage to red blood cells and hepatocytes, and this intoxication is considered a model for the induction of acute liver failure. In the present study, the hepatoprotective effects of coenzyme Q10 (CoQ10) and N-acetylcysteine (NAC) against experimental paracetamol (PAR) poisoning were analysed. Thirty-five adult Wistar rats (Rattus novergicus albinus) were randomly assigned to five groups, and thirty-one of these survived the treatments. Negative control group (CON-) received 1mL of 0.9% NaCl orally (PO). Other groups received 1.2g/kg of PAR (PO). Positive control group (CON+) received only PAR. NAC group received 800 mg/kg intraperitoneally (IP) of NAC 1h after the administration of PAR and at 12 h received 1mL of 0.9% NaCl, IP. The fourth group (CoQ10) received 1h and 12 h after intoxication, CoQ10 (10mg/kg IP). And the fifth group (NAC+CoQ10) received NAC (800mg/kg, IP) and CoQ10 (10mg/kg, IP). After 12 hours, the rats were euthanized and necropsied to collect liver and kidney tissues for histopathological evaluation and electronic microscopy. A single dose of PAR caused severe acute hepatitis. NAC couldn’t reverse the liver and kidney damages. The group that received CoQ10 and NAC had moderate liver damage, while the group that received only CoQ10 had lower values of liver enzymes and mild liver and kidney damage. Animals that received treatment with CoQ10 or NAC+CoQ10 presented normal hepatocyte mitochondria and nuclei. Although CoQ10 couldn’t reverse PAR organ damage, results indicate promising hepatoprotection in Wistar rats.
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spelling pubmed-104438532023-08-23 Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats da Silva, Rayanne Henrique Santana de Moura, Mariana de Paula, Larissa Arantes, Kelly Carolina da Silva, Marina de Amorim, Jaqueline Miguel, Marina Pacheco Martins, Danieli Brolo de Melo e Silva, Daniela Melo, Marília Martins Botelho, Ana Flávia Machado PLoS One Research Article Paracetamol (PAR) is a drug widely used in human and veterinary medicine as an analgesic and antipyretic, often involved in cases of intoxication. The most common clinical signs result from damage to red blood cells and hepatocytes, and this intoxication is considered a model for the induction of acute liver failure. In the present study, the hepatoprotective effects of coenzyme Q10 (CoQ10) and N-acetylcysteine (NAC) against experimental paracetamol (PAR) poisoning were analysed. Thirty-five adult Wistar rats (Rattus novergicus albinus) were randomly assigned to five groups, and thirty-one of these survived the treatments. Negative control group (CON-) received 1mL of 0.9% NaCl orally (PO). Other groups received 1.2g/kg of PAR (PO). Positive control group (CON+) received only PAR. NAC group received 800 mg/kg intraperitoneally (IP) of NAC 1h after the administration of PAR and at 12 h received 1mL of 0.9% NaCl, IP. The fourth group (CoQ10) received 1h and 12 h after intoxication, CoQ10 (10mg/kg IP). And the fifth group (NAC+CoQ10) received NAC (800mg/kg, IP) and CoQ10 (10mg/kg, IP). After 12 hours, the rats were euthanized and necropsied to collect liver and kidney tissues for histopathological evaluation and electronic microscopy. A single dose of PAR caused severe acute hepatitis. NAC couldn’t reverse the liver and kidney damages. The group that received CoQ10 and NAC had moderate liver damage, while the group that received only CoQ10 had lower values of liver enzymes and mild liver and kidney damage. Animals that received treatment with CoQ10 or NAC+CoQ10 presented normal hepatocyte mitochondria and nuclei. Although CoQ10 couldn’t reverse PAR organ damage, results indicate promising hepatoprotection in Wistar rats. Public Library of Science 2023-08-22 /pmc/articles/PMC10443853/ /pubmed/37607187 http://dx.doi.org/10.1371/journal.pone.0290268 Text en © 2023 da Silva et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
da Silva, Rayanne Henrique Santana
de Moura, Mariana
de Paula, Larissa
Arantes, Kelly Carolina
da Silva, Marina
de Amorim, Jaqueline
Miguel, Marina Pacheco
Martins, Danieli Brolo
de Melo e Silva, Daniela
Melo, Marília Martins
Botelho, Ana Flávia Machado
Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats
title Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats
title_full Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats
title_fullStr Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats
title_full_unstemmed Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats
title_short Effects of coenzyme Q10 and N-acetylcysteine on experimental poisoning by paracetamol in Wistar rats
title_sort effects of coenzyme q10 and n-acetylcysteine on experimental poisoning by paracetamol in wistar rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443853/
https://www.ncbi.nlm.nih.gov/pubmed/37607187
http://dx.doi.org/10.1371/journal.pone.0290268
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