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The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical st...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444840/ https://www.ncbi.nlm.nih.gov/pubmed/37608122 http://dx.doi.org/10.1038/s41598-023-41019-2 |
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author | Shigemi, Shohei Sato, Tadasu Sakamoto, Mayuri Yajima, Takehiro Honda, Takahiro Tsumaki, Hiroka Deguchi, Toru Ichikawa, Hiroyuki Fukunaga, Tomohiro Mizoguchi, Itaru |
author_facet | Shigemi, Shohei Sato, Tadasu Sakamoto, Mayuri Yajima, Takehiro Honda, Takahiro Tsumaki, Hiroka Deguchi, Toru Ichikawa, Hiroyuki Fukunaga, Tomohiro Mizoguchi, Itaru |
author_sort | Shigemi, Shohei |
collection | PubMed |
description | When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement. |
format | Online Article Text |
id | pubmed-10444840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104448402023-08-24 The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats Shigemi, Shohei Sato, Tadasu Sakamoto, Mayuri Yajima, Takehiro Honda, Takahiro Tsumaki, Hiroka Deguchi, Toru Ichikawa, Hiroyuki Fukunaga, Tomohiro Mizoguchi, Itaru Sci Rep Article When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement. Nature Publishing Group UK 2023-08-22 /pmc/articles/PMC10444840/ /pubmed/37608122 http://dx.doi.org/10.1038/s41598-023-41019-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Shigemi, Shohei Sato, Tadasu Sakamoto, Mayuri Yajima, Takehiro Honda, Takahiro Tsumaki, Hiroka Deguchi, Toru Ichikawa, Hiroyuki Fukunaga, Tomohiro Mizoguchi, Itaru The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
title | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
title_full | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
title_fullStr | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
title_full_unstemmed | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
title_short | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
title_sort | role of trpv2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444840/ https://www.ncbi.nlm.nih.gov/pubmed/37608122 http://dx.doi.org/10.1038/s41598-023-41019-2 |
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