Cargando…

The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats

When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical st...

Descripción completa

Detalles Bibliográficos
Autores principales: Shigemi, Shohei, Sato, Tadasu, Sakamoto, Mayuri, Yajima, Takehiro, Honda, Takahiro, Tsumaki, Hiroka, Deguchi, Toru, Ichikawa, Hiroyuki, Fukunaga, Tomohiro, Mizoguchi, Itaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444840/
https://www.ncbi.nlm.nih.gov/pubmed/37608122
http://dx.doi.org/10.1038/s41598-023-41019-2
_version_ 1785094042174881792
author Shigemi, Shohei
Sato, Tadasu
Sakamoto, Mayuri
Yajima, Takehiro
Honda, Takahiro
Tsumaki, Hiroka
Deguchi, Toru
Ichikawa, Hiroyuki
Fukunaga, Tomohiro
Mizoguchi, Itaru
author_facet Shigemi, Shohei
Sato, Tadasu
Sakamoto, Mayuri
Yajima, Takehiro
Honda, Takahiro
Tsumaki, Hiroka
Deguchi, Toru
Ichikawa, Hiroyuki
Fukunaga, Tomohiro
Mizoguchi, Itaru
author_sort Shigemi, Shohei
collection PubMed
description When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement.
format Online
Article
Text
id pubmed-10444840
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-104448402023-08-24 The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats Shigemi, Shohei Sato, Tadasu Sakamoto, Mayuri Yajima, Takehiro Honda, Takahiro Tsumaki, Hiroka Deguchi, Toru Ichikawa, Hiroyuki Fukunaga, Tomohiro Mizoguchi, Itaru Sci Rep Article When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement. Nature Publishing Group UK 2023-08-22 /pmc/articles/PMC10444840/ /pubmed/37608122 http://dx.doi.org/10.1038/s41598-023-41019-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shigemi, Shohei
Sato, Tadasu
Sakamoto, Mayuri
Yajima, Takehiro
Honda, Takahiro
Tsumaki, Hiroka
Deguchi, Toru
Ichikawa, Hiroyuki
Fukunaga, Tomohiro
Mizoguchi, Itaru
The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
title The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
title_full The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
title_fullStr The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
title_full_unstemmed The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
title_short The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
title_sort role of trpv2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444840/
https://www.ncbi.nlm.nih.gov/pubmed/37608122
http://dx.doi.org/10.1038/s41598-023-41019-2
work_keys_str_mv AT shigemishohei theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT satotadasu theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT sakamotomayuri theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT yajimatakehiro theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT hondatakahiro theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT tsumakihiroka theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT deguchitoru theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT ichikawahiroyuki theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT fukunagatomohiro theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT mizoguchiitaru theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT shigemishohei roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT satotadasu roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT sakamotomayuri roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT yajimatakehiro roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT hondatakahiro roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT tsumakihiroka roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT deguchitoru roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT ichikawahiroyuki roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT fukunagatomohiro roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats
AT mizoguchiitaru roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats