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Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography

Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflec...

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Autores principales: Kang, Sungsam, Zhou, Renjie, Brelen, Marten, Mak, Heather K., Lin, Yuechuan, So, Peter T. C., Yaqoob, Zahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444882/
https://www.ncbi.nlm.nih.gov/pubmed/37607903
http://dx.doi.org/10.1038/s41377-023-01240-0
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author Kang, Sungsam
Zhou, Renjie
Brelen, Marten
Mak, Heather K.
Lin, Yuechuan
So, Peter T. C.
Yaqoob, Zahid
author_facet Kang, Sungsam
Zhou, Renjie
Brelen, Marten
Mak, Heather K.
Lin, Yuechuan
So, Peter T. C.
Yaqoob, Zahid
author_sort Kang, Sungsam
collection PubMed
description Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflection geometry. In SDT, multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry, while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations. Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT, we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths. Most importantly, we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties, including the mapping of nanoscale topographic features of Dua’s and Descemet’s membranes that had not been previously visualized.
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spelling pubmed-104448822023-08-24 Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography Kang, Sungsam Zhou, Renjie Brelen, Marten Mak, Heather K. Lin, Yuechuan So, Peter T. C. Yaqoob, Zahid Light Sci Appl Article Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflection geometry. In SDT, multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry, while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations. Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT, we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths. Most importantly, we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties, including the mapping of nanoscale topographic features of Dua’s and Descemet’s membranes that had not been previously visualized. Nature Publishing Group UK 2023-08-22 /pmc/articles/PMC10444882/ /pubmed/37607903 http://dx.doi.org/10.1038/s41377-023-01240-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kang, Sungsam
Zhou, Renjie
Brelen, Marten
Mak, Heather K.
Lin, Yuechuan
So, Peter T. C.
Yaqoob, Zahid
Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
title Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
title_full Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
title_fullStr Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
title_full_unstemmed Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
title_short Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
title_sort mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444882/
https://www.ncbi.nlm.nih.gov/pubmed/37607903
http://dx.doi.org/10.1038/s41377-023-01240-0
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