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SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility
The cytoplasmic droplet is a conserved dilated area of cytoplasm situated at the neck of the sperm flagellum. Viewed as residual cytoplasm inherited from late spermatids, the cytoplasmic droplet contains numerous saccular elements as its key content. However, the origin of these saccules and the fun...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444883/ https://www.ncbi.nlm.nih.gov/pubmed/37607933 http://dx.doi.org/10.1038/s41467-023-40862-1 |
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author | Liu, Jiali Hermo, Louis Ding, Deqiang Wei, Chao Mann, Jeffrey M. Yan, Xiaoyuan Melnick, Ashley F. Wu, Yingjie Withrow, Alicia Cibelli, Jose Hess, Rex A. Chen, Chen |
author_facet | Liu, Jiali Hermo, Louis Ding, Deqiang Wei, Chao Mann, Jeffrey M. Yan, Xiaoyuan Melnick, Ashley F. Wu, Yingjie Withrow, Alicia Cibelli, Jose Hess, Rex A. Chen, Chen |
author_sort | Liu, Jiali |
collection | PubMed |
description | The cytoplasmic droplet is a conserved dilated area of cytoplasm situated at the neck of the sperm flagellum. Viewed as residual cytoplasm inherited from late spermatids, the cytoplasmic droplet contains numerous saccular elements as its key content. However, the origin of these saccules and the function of the cytoplasmic droplet have long been speculative. Here, we identify the molecular origin of these cytoplasmic droplet components by uncovering a vesicle pathway essential for formation and sequestration of saccules within the cytoplasmic droplet. This process is governed by a transmembrane protein SYPL1 and its interaction with VAMP3. Genetic ablation of SYPL1 in mice reveals that SYPL1 dictates the formation and accumulation of saccular elements in the forming cytoplasmic droplet. Derived from the Golgi, SYPL1 vesicles are critical for segregation of key metabolic enzymes within the forming cytoplasmic droplet of late spermatids and epididymal sperm, which are required for sperm development and male fertility. Our results uncover a mechanism to actively form and segregate saccules within the cytoplasmic droplet to promote sperm fertility. |
format | Online Article Text |
id | pubmed-10444883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104448832023-08-24 SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility Liu, Jiali Hermo, Louis Ding, Deqiang Wei, Chao Mann, Jeffrey M. Yan, Xiaoyuan Melnick, Ashley F. Wu, Yingjie Withrow, Alicia Cibelli, Jose Hess, Rex A. Chen, Chen Nat Commun Article The cytoplasmic droplet is a conserved dilated area of cytoplasm situated at the neck of the sperm flagellum. Viewed as residual cytoplasm inherited from late spermatids, the cytoplasmic droplet contains numerous saccular elements as its key content. However, the origin of these saccules and the function of the cytoplasmic droplet have long been speculative. Here, we identify the molecular origin of these cytoplasmic droplet components by uncovering a vesicle pathway essential for formation and sequestration of saccules within the cytoplasmic droplet. This process is governed by a transmembrane protein SYPL1 and its interaction with VAMP3. Genetic ablation of SYPL1 in mice reveals that SYPL1 dictates the formation and accumulation of saccular elements in the forming cytoplasmic droplet. Derived from the Golgi, SYPL1 vesicles are critical for segregation of key metabolic enzymes within the forming cytoplasmic droplet of late spermatids and epididymal sperm, which are required for sperm development and male fertility. Our results uncover a mechanism to actively form and segregate saccules within the cytoplasmic droplet to promote sperm fertility. Nature Publishing Group UK 2023-08-22 /pmc/articles/PMC10444883/ /pubmed/37607933 http://dx.doi.org/10.1038/s41467-023-40862-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Liu, Jiali Hermo, Louis Ding, Deqiang Wei, Chao Mann, Jeffrey M. Yan, Xiaoyuan Melnick, Ashley F. Wu, Yingjie Withrow, Alicia Cibelli, Jose Hess, Rex A. Chen, Chen SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
title | SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
title_full | SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
title_fullStr | SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
title_full_unstemmed | SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
title_short | SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
title_sort | sypl1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444883/ https://www.ncbi.nlm.nih.gov/pubmed/37607933 http://dx.doi.org/10.1038/s41467-023-40862-1 |
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