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A functional sgRNA-CRISPR screening method for generating murine RET and NTRK1 rearranged oncogenes

CRISPR/Cas9 gene editing represents a powerful tool for investigating fusion oncogenes in cancer biology. Successful experiments require that sgRNAs correctly associate with their target sequence and initiate double stranded breaks which are subsequently repaired by endogenous DNA repair systems yie...

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Detalles Bibliográficos
Autores principales:	Schubert, Laura, Le, Anh T., Hinz, Trista K., Navarro, Andre C., Nelson-Taylor, Sarah K., Nemenoff, Raphael A., Heasley, Lynn E., Doebele, Robert C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Company of Biologists Ltd 2023
Materias:	Methods & Techniques
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445739/ https://www.ncbi.nlm.nih.gov/pubmed/37470475 http://dx.doi.org/10.1242/bio.059994

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