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Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase

Accurate genome segregation in mitosis requires that all chromosomes are bioriented on the spindle. Cells monitor biorientation by sensing tension across sister centromeres. Chromosomes that are not bioriented have low centromere tension, which allows Aurora B (yeast Ipl1) to perform error correctio...

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Autores principales: Edgerton, Heather D., Mukherjee, Soumya, Johansson, Marnie, Bachant, Jeff, Gardner, Melissa K., Clarke, Duncan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445749/
https://www.ncbi.nlm.nih.gov/pubmed/37519149
http://dx.doi.org/10.1242/jcs.261416
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author Edgerton, Heather D.
Mukherjee, Soumya
Johansson, Marnie
Bachant, Jeff
Gardner, Melissa K.
Clarke, Duncan J.
author_facet Edgerton, Heather D.
Mukherjee, Soumya
Johansson, Marnie
Bachant, Jeff
Gardner, Melissa K.
Clarke, Duncan J.
author_sort Edgerton, Heather D.
collection PubMed
description Accurate genome segregation in mitosis requires that all chromosomes are bioriented on the spindle. Cells monitor biorientation by sensing tension across sister centromeres. Chromosomes that are not bioriented have low centromere tension, which allows Aurora B (yeast Ipl1) to perform error correction that locally loosens kinetochore–microtubule attachments to allow detachment of microtubules and fresh attempts at achieving biorientation. However, it is not known whether low tension recruits Aurora B to centromeres or, alternatively, whether low tension directly activates Aurora B already localized at centromeres. In this work, we experimentally induced low tension in metaphase Saccharomyces cerevisiae yeast cells, then monitored Ipl1 localization. We find low tension recruits Ipl1 to centromeres. Furthermore, low tension-induced Ipl1 recruitment depended on Bub1, which is known to provide a binding site for Ipl1. In contrast, Top2, which can also recruit Ipl1 to centromeres, was not required. Our results demonstrate cells are sensitive to low tension at centromeres and respond by actively recruiting Ip1l for error correction.
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spelling pubmed-104457492023-08-24 Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase Edgerton, Heather D. Mukherjee, Soumya Johansson, Marnie Bachant, Jeff Gardner, Melissa K. Clarke, Duncan J. J Cell Sci Research Article Accurate genome segregation in mitosis requires that all chromosomes are bioriented on the spindle. Cells monitor biorientation by sensing tension across sister centromeres. Chromosomes that are not bioriented have low centromere tension, which allows Aurora B (yeast Ipl1) to perform error correction that locally loosens kinetochore–microtubule attachments to allow detachment of microtubules and fresh attempts at achieving biorientation. However, it is not known whether low tension recruits Aurora B to centromeres or, alternatively, whether low tension directly activates Aurora B already localized at centromeres. In this work, we experimentally induced low tension in metaphase Saccharomyces cerevisiae yeast cells, then monitored Ipl1 localization. We find low tension recruits Ipl1 to centromeres. Furthermore, low tension-induced Ipl1 recruitment depended on Bub1, which is known to provide a binding site for Ipl1. In contrast, Top2, which can also recruit Ipl1 to centromeres, was not required. Our results demonstrate cells are sensitive to low tension at centromeres and respond by actively recruiting Ip1l for error correction. The Company of Biologists Ltd 2023-08-17 /pmc/articles/PMC10445749/ /pubmed/37519149 http://dx.doi.org/10.1242/jcs.261416 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Edgerton, Heather D.
Mukherjee, Soumya
Johansson, Marnie
Bachant, Jeff
Gardner, Melissa K.
Clarke, Duncan J.
Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
title Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
title_full Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
title_fullStr Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
title_full_unstemmed Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
title_short Low tension recruits the yeast Aurora B protein Ipl1 to centromeres in metaphase
title_sort low tension recruits the yeast aurora b protein ipl1 to centromeres in metaphase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445749/
https://www.ncbi.nlm.nih.gov/pubmed/37519149
http://dx.doi.org/10.1242/jcs.261416
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