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Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage

The invasive trophoblast cell lineages in rat and human share crucial responsibilities in establishing the uterine-placental interface of the hemochorial placenta. These observations have led to the rat becoming an especially useful animal model for studying hemochorial placentation. However, our un...

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Autores principales: Vu, Ha T. H., Scott, Regan L., Iqbal, Khursheed, Soares, Michael J., Tuteja, Geetu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445752/
https://www.ncbi.nlm.nih.gov/pubmed/37417811
http://dx.doi.org/10.1242/dev.201826
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author Vu, Ha T. H.
Scott, Regan L.
Iqbal, Khursheed
Soares, Michael J.
Tuteja, Geetu
author_facet Vu, Ha T. H.
Scott, Regan L.
Iqbal, Khursheed
Soares, Michael J.
Tuteja, Geetu
author_sort Vu, Ha T. H.
collection PubMed
description The invasive trophoblast cell lineages in rat and human share crucial responsibilities in establishing the uterine-placental interface of the hemochorial placenta. These observations have led to the rat becoming an especially useful animal model for studying hemochorial placentation. However, our understanding of similarities or differences between regulatory mechanisms governing rat and human invasive trophoblast cell populations is limited. In this study, we generated single-nucleus ATAC-seq data from gestation day 15.5 and 19.5 rat uterine-placental interface tissues, and integrated the data with single-cell RNA-seq data generated at the same stages. We determined the chromatin accessibility profiles of invasive trophoblast, natural killer, macrophage, endothelial and smooth muscle cells, and compared invasive trophoblast chromatin accessibility with extravillous trophoblast cell accessibility. In comparing chromatin accessibility profiles between species, we found similarities in patterns of gene regulation and groups of motifs enriched in accessible regions. Finally, we identified a conserved gene regulatory network in invasive trophoblast cells. Our data, findings and analysis will facilitate future studies investigating regulatory mechanisms essential for the invasive trophoblast cell lineage.
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spelling pubmed-104457522023-08-24 Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage Vu, Ha T. H. Scott, Regan L. Iqbal, Khursheed Soares, Michael J. Tuteja, Geetu Development Techniques and Resources The invasive trophoblast cell lineages in rat and human share crucial responsibilities in establishing the uterine-placental interface of the hemochorial placenta. These observations have led to the rat becoming an especially useful animal model for studying hemochorial placentation. However, our understanding of similarities or differences between regulatory mechanisms governing rat and human invasive trophoblast cell populations is limited. In this study, we generated single-nucleus ATAC-seq data from gestation day 15.5 and 19.5 rat uterine-placental interface tissues, and integrated the data with single-cell RNA-seq data generated at the same stages. We determined the chromatin accessibility profiles of invasive trophoblast, natural killer, macrophage, endothelial and smooth muscle cells, and compared invasive trophoblast chromatin accessibility with extravillous trophoblast cell accessibility. In comparing chromatin accessibility profiles between species, we found similarities in patterns of gene regulation and groups of motifs enriched in accessible regions. Finally, we identified a conserved gene regulatory network in invasive trophoblast cells. Our data, findings and analysis will facilitate future studies investigating regulatory mechanisms essential for the invasive trophoblast cell lineage. The Company of Biologists Ltd 2023-07-31 /pmc/articles/PMC10445752/ /pubmed/37417811 http://dx.doi.org/10.1242/dev.201826 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Techniques and Resources
Vu, Ha T. H.
Scott, Regan L.
Iqbal, Khursheed
Soares, Michael J.
Tuteja, Geetu
Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
title Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
title_full Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
title_fullStr Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
title_full_unstemmed Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
title_short Core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
title_sort core conserved transcriptional regulatory networks define the invasive trophoblast cell lineage
topic Techniques and Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10445752/
https://www.ncbi.nlm.nih.gov/pubmed/37417811
http://dx.doi.org/10.1242/dev.201826
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