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Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction

BACKGROUND: Tregs plays a critical role in the development of secondary injuries in diseases. Accumulating evidence suggests an association between ischemic stroke and renal dysfunction; however, the underlying mechanisms remain unclear. This study aimed to investigate the potential of Tregs in inhi...

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Autores principales: Wang, Shuai, Shi, Yubin, Zhang, Yanqi, Yuan, Fengyun, Mao, Mintao, Ma, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10446222/
https://www.ncbi.nlm.nih.gov/pubmed/37622110
http://dx.doi.org/10.3389/fimmu.2023.1255316
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author Wang, Shuai
Shi, Yubin
Zhang, Yanqi
Yuan, Fengyun
Mao, Mintao
Ma, Jun
author_facet Wang, Shuai
Shi, Yubin
Zhang, Yanqi
Yuan, Fengyun
Mao, Mintao
Ma, Jun
author_sort Wang, Shuai
collection PubMed
description BACKGROUND: Tregs plays a critical role in the development of secondary injuries in diseases. Accumulating evidence suggests an association between ischemic stroke and renal dysfunction; however, the underlying mechanisms remain unclear. This study aimed to investigate the potential of Tregs in inhibiting the activation of astrocytes after focal cerebral infarction. METHODS: This study aimed to investigate the renal consequences of focal cerebral ischemia by subjecting a mouse model to transient middle cerebral artery occlusion (tMCAO). Subsequently, we assessed renal fibrosis, renal ferroptosis, Treg infiltration, astrocyte activation, as well as the expression levels of active GPX4, FSP1, IL-10, IL-6, and IL-2 after a 2-week period. RESULTS: In the tMCAO mouse model, depletion of tregs protected against activation of astrocyte and significantly decreased FSP1, IL-6, IL-2, and NLRP3 expression levels, while partially reversing the changes in Tregs. Mechanistically, tregs depletion attenuates renal fibrosis by modulating IL-10/GPX4 following cerebral infarction. CONCLUSION: Tregs depletion attenuates renal fibrosis by modulating IL-10/GPX4 following cerebral infarction.
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spelling pubmed-104462222023-08-24 Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction Wang, Shuai Shi, Yubin Zhang, Yanqi Yuan, Fengyun Mao, Mintao Ma, Jun Front Immunol Immunology BACKGROUND: Tregs plays a critical role in the development of secondary injuries in diseases. Accumulating evidence suggests an association between ischemic stroke and renal dysfunction; however, the underlying mechanisms remain unclear. This study aimed to investigate the potential of Tregs in inhibiting the activation of astrocytes after focal cerebral infarction. METHODS: This study aimed to investigate the renal consequences of focal cerebral ischemia by subjecting a mouse model to transient middle cerebral artery occlusion (tMCAO). Subsequently, we assessed renal fibrosis, renal ferroptosis, Treg infiltration, astrocyte activation, as well as the expression levels of active GPX4, FSP1, IL-10, IL-6, and IL-2 after a 2-week period. RESULTS: In the tMCAO mouse model, depletion of tregs protected against activation of astrocyte and significantly decreased FSP1, IL-6, IL-2, and NLRP3 expression levels, while partially reversing the changes in Tregs. Mechanistically, tregs depletion attenuates renal fibrosis by modulating IL-10/GPX4 following cerebral infarction. CONCLUSION: Tregs depletion attenuates renal fibrosis by modulating IL-10/GPX4 following cerebral infarction. Frontiers Media S.A. 2023-08-09 /pmc/articles/PMC10446222/ /pubmed/37622110 http://dx.doi.org/10.3389/fimmu.2023.1255316 Text en Copyright © 2023 Wang, Shi, Zhang, Yuan, Mao and Ma https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wang, Shuai
Shi, Yubin
Zhang, Yanqi
Yuan, Fengyun
Mao, Mintao
Ma, Jun
Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction
title Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction
title_full Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction
title_fullStr Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction
title_full_unstemmed Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction
title_short Tregs depletion aggravates activation of astrocytes by modulating IL-10/GXP4 following cerebral infarction
title_sort tregs depletion aggravates activation of astrocytes by modulating il-10/gxp4 following cerebral infarction
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10446222/
https://www.ncbi.nlm.nih.gov/pubmed/37622110
http://dx.doi.org/10.3389/fimmu.2023.1255316
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