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The genesis of human hematopoietic stem cells

Developmental hematopoiesis consists of multiple, partially overlapping hematopoietic waves that generate the differentiated blood cells required for embryonic development while establishing a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. This multilayered design in wh...

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Detalles Bibliográficos
Autores principales: Calvanese, Vincenzo, Mikkola, Hanna K. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Hematology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10447622/
https://www.ncbi.nlm.nih.gov/pubmed/37339578
http://dx.doi.org/10.1182/blood.2022017934
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author Calvanese, Vincenzo
Mikkola, Hanna K. A.
author_facet Calvanese, Vincenzo
Mikkola, Hanna K. A.
author_sort Calvanese, Vincenzo
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description Developmental hematopoiesis consists of multiple, partially overlapping hematopoietic waves that generate the differentiated blood cells required for embryonic development while establishing a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. This multilayered design in which active hematopoiesis migrates through diverse extra and intraembryonic tissues has made it difficult to define a roadmap for generating HSCs vs non–self-renewing progenitors, especially in humans. Recent single-cell studies have helped in identifying the rare human HSCs at stages when functional assays are unsuitable for distinguishing them from progenitors. This approach has made it possible to track the origin of human HSCs to the unique type of arterial endothelium in the aorta-gonad-mesonephros region and document novel benchmarks for HSC migration and maturation in the conceptus. These studies have delivered new insights into the intricate process of HSC generation and provided tools to inform the in vitro efforts to replicate the physiological developmental journey from pluripotent stem cells via distinct mesodermal and endothelial intermediates to HSCs.
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spelling pubmed-104476222023-08-25 The genesis of human hematopoietic stem cells Calvanese, Vincenzo Mikkola, Hanna K. A. Blood Hematopoietic Stem Cells Developmental hematopoiesis consists of multiple, partially overlapping hematopoietic waves that generate the differentiated blood cells required for embryonic development while establishing a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. This multilayered design in which active hematopoiesis migrates through diverse extra and intraembryonic tissues has made it difficult to define a roadmap for generating HSCs vs non–self-renewing progenitors, especially in humans. Recent single-cell studies have helped in identifying the rare human HSCs at stages when functional assays are unsuitable for distinguishing them from progenitors. This approach has made it possible to track the origin of human HSCs to the unique type of arterial endothelium in the aorta-gonad-mesonephros region and document novel benchmarks for HSC migration and maturation in the conceptus. These studies have delivered new insights into the intricate process of HSC generation and provided tools to inform the in vitro efforts to replicate the physiological developmental journey from pluripotent stem cells via distinct mesodermal and endothelial intermediates to HSCs. The American Society of Hematology 2023-08-10 2023-02-20 /pmc/articles/PMC10447622/ /pubmed/37339578 http://dx.doi.org/10.1182/blood.2022017934 Text en © 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Hematopoietic Stem Cells
Calvanese, Vincenzo
Mikkola, Hanna K. A.
The genesis of human hematopoietic stem cells
title The genesis of human hematopoietic stem cells
title_full The genesis of human hematopoietic stem cells
title_fullStr The genesis of human hematopoietic stem cells
title_full_unstemmed The genesis of human hematopoietic stem cells
title_short The genesis of human hematopoietic stem cells
title_sort genesis of human hematopoietic stem cells
topic Hematopoietic Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10447622/
https://www.ncbi.nlm.nih.gov/pubmed/37339578
http://dx.doi.org/10.1182/blood.2022017934
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