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Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas
Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons. Pseudomonas spp. have broad metabolic potential for degrading aromatic compounds. The objective of this study was to develop a “biological funneling” s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448021/ https://www.ncbi.nlm.nih.gov/pubmed/37637202 http://dx.doi.org/10.1016/j.synbio.2023.08.001 |
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author | He, Siyang Wang, Weiwei Wang, Weidong Hu, Haiyang Xu, Ping Tang, Hongzhi |
author_facet | He, Siyang Wang, Weiwei Wang, Weidong Hu, Haiyang Xu, Ping Tang, Hongzhi |
author_sort | He, Siyang |
collection | PubMed |
description | Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons. Pseudomonas spp. have broad metabolic potential for degrading aromatic compounds. The objective of this study was to develop a “biological funneling” strategy based on genetic modification to convert complex aromatic compounds into cis,cis-muconate (ccMA) using Pseudomonas putida B6-2 and P. brassicacearum MPDS as biocatalysts. The engineered strains B6-2 (B6-2ΔcatBΔsalC) and MPDS (MPDSΔsalC(pUCP18k-catA)) thrived with biphenyl or naphthalene as the sole carbon source and produced ccMA, attaining molar conversions of 95.3% (ccMA/biphenyl) and 100% (ccMA/naphthalene). Under mixed substrates, B6-2ΔcatBΔsalC grew on biphenyl as a carbon source and transformed ccMA from non-growth substrates benzoate or salicylate to obtain higher product concentration. Inserting exogenous clusters like bedDC(1)C(2)AB and xylCMAB allowed B6-2 recombinant strains to convert benzene and toluene to ccMA. In mixed substrates, constructed consortia of engineered strains B6-2 and MPDS specialized in catabolism of biphenyl and naphthalene; the highest molar conversion rate of ccMA from mixed substrates was 85.2% when B6-2ΔcatBΔsalC was added after 24 h of MPDSΔsalC(pUCP18k-catA) incubation with biphenyl and naphthalene. This study provides worthwhile insights into efficient production of ccMA from aromatic hydrocarbons by reusing complex pollutants. |
format | Online Article Text |
id | pubmed-10448021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-104480212023-08-25 Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas He, Siyang Wang, Weiwei Wang, Weidong Hu, Haiyang Xu, Ping Tang, Hongzhi Synth Syst Biotechnol Article Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons. Pseudomonas spp. have broad metabolic potential for degrading aromatic compounds. The objective of this study was to develop a “biological funneling” strategy based on genetic modification to convert complex aromatic compounds into cis,cis-muconate (ccMA) using Pseudomonas putida B6-2 and P. brassicacearum MPDS as biocatalysts. The engineered strains B6-2 (B6-2ΔcatBΔsalC) and MPDS (MPDSΔsalC(pUCP18k-catA)) thrived with biphenyl or naphthalene as the sole carbon source and produced ccMA, attaining molar conversions of 95.3% (ccMA/biphenyl) and 100% (ccMA/naphthalene). Under mixed substrates, B6-2ΔcatBΔsalC grew on biphenyl as a carbon source and transformed ccMA from non-growth substrates benzoate or salicylate to obtain higher product concentration. Inserting exogenous clusters like bedDC(1)C(2)AB and xylCMAB allowed B6-2 recombinant strains to convert benzene and toluene to ccMA. In mixed substrates, constructed consortia of engineered strains B6-2 and MPDS specialized in catabolism of biphenyl and naphthalene; the highest molar conversion rate of ccMA from mixed substrates was 85.2% when B6-2ΔcatBΔsalC was added after 24 h of MPDSΔsalC(pUCP18k-catA) incubation with biphenyl and naphthalene. This study provides worthwhile insights into efficient production of ccMA from aromatic hydrocarbons by reusing complex pollutants. KeAi Publishing 2023-08-09 /pmc/articles/PMC10448021/ /pubmed/37637202 http://dx.doi.org/10.1016/j.synbio.2023.08.001 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article He, Siyang Wang, Weiwei Wang, Weidong Hu, Haiyang Xu, Ping Tang, Hongzhi Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas |
title | Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas |
title_full | Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas |
title_fullStr | Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas |
title_full_unstemmed | Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas |
title_short | Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas |
title_sort | microbial production of cis,cis-muconic acid from aromatic compounds in engineered pseudomonas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448021/ https://www.ncbi.nlm.nih.gov/pubmed/37637202 http://dx.doi.org/10.1016/j.synbio.2023.08.001 |
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