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Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates

Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs). Here, we present a protocol to study rehabilitation techniques in NHPs using a photothrombotic technique, a...

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Autores principales: Stanis, Noah, Khateeb, Karam, Zhou, Jasmine, Wang, Ruikang K., Yazdan-Shahmorad, Azadeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448414/
https://www.ncbi.nlm.nih.gov/pubmed/37573501
http://dx.doi.org/10.1016/j.xpro.2023.102496
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author Stanis, Noah
Khateeb, Karam
Zhou, Jasmine
Wang, Ruikang K.
Yazdan-Shahmorad, Azadeh
author_facet Stanis, Noah
Khateeb, Karam
Zhou, Jasmine
Wang, Ruikang K.
Yazdan-Shahmorad, Azadeh
author_sort Stanis, Noah
collection PubMed
description Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs). Here, we present a protocol to study rehabilitation techniques in NHPs using a photothrombotic technique, a form of optical focal lesioning. We also describe steps for simultaneous neurophysiological recording and in vivo validation through vascular flow imaging. This interface can examine emerging neurorehabilitation strategies in the post-stroke environment in NHPs that are evolutionarily close to humans. For complete details on the use and execution of this protocol, please refer to Khateeb et al. (2022).(6)
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spelling pubmed-104484142023-08-25 Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates Stanis, Noah Khateeb, Karam Zhou, Jasmine Wang, Ruikang K. Yazdan-Shahmorad, Azadeh STAR Protoc Protocol Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs). Here, we present a protocol to study rehabilitation techniques in NHPs using a photothrombotic technique, a form of optical focal lesioning. We also describe steps for simultaneous neurophysiological recording and in vivo validation through vascular flow imaging. This interface can examine emerging neurorehabilitation strategies in the post-stroke environment in NHPs that are evolutionarily close to humans. For complete details on the use and execution of this protocol, please refer to Khateeb et al. (2022).(6) Elsevier 2023-08-11 /pmc/articles/PMC10448414/ /pubmed/37573501 http://dx.doi.org/10.1016/j.xpro.2023.102496 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Stanis, Noah
Khateeb, Karam
Zhou, Jasmine
Wang, Ruikang K.
Yazdan-Shahmorad, Azadeh
Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
title Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
title_full Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
title_fullStr Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
title_full_unstemmed Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
title_short Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
title_sort protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448414/
https://www.ncbi.nlm.nih.gov/pubmed/37573501
http://dx.doi.org/10.1016/j.xpro.2023.102496
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