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Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients
[Image: see text] For the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously me...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448438/ https://www.ncbi.nlm.nih.gov/pubmed/37486019 http://dx.doi.org/10.1021/acs.analchem.3c01982 |
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author | Dortez, Silvia DeGregorio-Rocasolano, Núria Millán, Mònica Gasull, Teresa Crevillen, Agustín G. Escarpa, Alberto |
author_facet | Dortez, Silvia DeGregorio-Rocasolano, Núria Millán, Mònica Gasull, Teresa Crevillen, Agustín G. Escarpa, Alberto |
author_sort | Dortez, Silvia |
collection | PubMed |
description | [Image: see text] For the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously measured on the same PAD using ferrozine as a chromophore and a smartphone as the color reader. To this end, Tf was first isolated from serum using anti-Tf immunomagnetic beads to ensure that only the Tf-bound iron was measured, improving the selectivity and accuracy of TSAT assessment. To demonstrate the practical utility of the device, it was validated by analyzing a certified reference material, showing excellent accuracy (E(r) < 4%) and good precision (RSD ≤ 6%). Finally, 18 diagnosed serum samples from ischemic stroke patients were analyzed by this approach, and the results were compared with those obtained by urea-PAGE, showing not only an excellent correlation (r = 0.93, p < 0.05) but that the PAD approach has become statistically identical to the free-interference urea-PAGE. In comparison with the slow, tedious, and non-miniaturized-PAGE, this PAD approach exhibited attractive characteristics such as low cost, disposability, and connectivity, showing great potential for future point-of-care testing, especially in developing countries and/or remote areas, where access to medical or clinical facilities is limited. |
format | Online Article Text |
id | pubmed-10448438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104484382023-08-25 Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients Dortez, Silvia DeGregorio-Rocasolano, Núria Millán, Mònica Gasull, Teresa Crevillen, Agustín G. Escarpa, Alberto Anal Chem [Image: see text] For the first time, a paper-based analytical device (PAD) was developed for the assessment of transferrin saturation (TSAT), which is defined as the ratio between iron bound to transferrin (Tf) and the total iron-binding capacity (TIBC) of Tf. Both parameters were simultaneously measured on the same PAD using ferrozine as a chromophore and a smartphone as the color reader. To this end, Tf was first isolated from serum using anti-Tf immunomagnetic beads to ensure that only the Tf-bound iron was measured, improving the selectivity and accuracy of TSAT assessment. To demonstrate the practical utility of the device, it was validated by analyzing a certified reference material, showing excellent accuracy (E(r) < 4%) and good precision (RSD ≤ 6%). Finally, 18 diagnosed serum samples from ischemic stroke patients were analyzed by this approach, and the results were compared with those obtained by urea-PAGE, showing not only an excellent correlation (r = 0.93, p < 0.05) but that the PAD approach has become statistically identical to the free-interference urea-PAGE. In comparison with the slow, tedious, and non-miniaturized-PAGE, this PAD approach exhibited attractive characteristics such as low cost, disposability, and connectivity, showing great potential for future point-of-care testing, especially in developing countries and/or remote areas, where access to medical or clinical facilities is limited. American Chemical Society 2023-07-24 /pmc/articles/PMC10448438/ /pubmed/37486019 http://dx.doi.org/10.1021/acs.analchem.3c01982 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Dortez, Silvia DeGregorio-Rocasolano, Núria Millán, Mònica Gasull, Teresa Crevillen, Agustín G. Escarpa, Alberto Paper-Based Analytical Devices for Accurate Assessment of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic Stroke Patients |
title | Paper-Based Analytical Devices for Accurate Assessment
of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic
Stroke Patients |
title_full | Paper-Based Analytical Devices for Accurate Assessment
of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic
Stroke Patients |
title_fullStr | Paper-Based Analytical Devices for Accurate Assessment
of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic
Stroke Patients |
title_full_unstemmed | Paper-Based Analytical Devices for Accurate Assessment
of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic
Stroke Patients |
title_short | Paper-Based Analytical Devices for Accurate Assessment
of Transferrin Saturation in Diagnosed Clinical Samples from Ischemic
Stroke Patients |
title_sort | paper-based analytical devices for accurate assessment
of transferrin saturation in diagnosed clinical samples from ischemic
stroke patients |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448438/ https://www.ncbi.nlm.nih.gov/pubmed/37486019 http://dx.doi.org/10.1021/acs.analchem.3c01982 |
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