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Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst

Soybean (Glycine max) is an increasingly relevant crop due to its economic importance and also a model plant for the study of root symbiotic associations with nodule forming rhizobia. Plant polyesters mediate plant-microbe interactions with both pathogenic and beneficial microbes; suberin has been h...

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Autores principales: Escórcio, Rita, Sandhu, Armaan K., Bento, Artur, Tomé, Ana S., Moreira, Carlos J. S., Brözel, Volker S., Silva Pereira, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448529/
https://www.ncbi.nlm.nih.gov/pubmed/37638099
http://dx.doi.org/10.3389/fchem.2023.1165234
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author Escórcio, Rita
Sandhu, Armaan K.
Bento, Artur
Tomé, Ana S.
Moreira, Carlos J. S.
Brözel, Volker S.
Silva Pereira, Cristina
author_facet Escórcio, Rita
Sandhu, Armaan K.
Bento, Artur
Tomé, Ana S.
Moreira, Carlos J. S.
Brözel, Volker S.
Silva Pereira, Cristina
author_sort Escórcio, Rita
collection PubMed
description Soybean (Glycine max) is an increasingly relevant crop due to its economic importance and also a model plant for the study of root symbiotic associations with nodule forming rhizobia. Plant polyesters mediate plant-microbe interactions with both pathogenic and beneficial microbes; suberin has been hypothesized to play a key role during the early steps of rhizobia attachment to the root. The downside is that suberin chemistry in soybean root is still scarcely studied. This study addresses this outstanding question by reporting a straightforward workflow for a speedy purification of suberin from soybean root and for its subsequent detailed chemical analysis. To purify suberin, cholinium hexanoate (an ionic liquid) was used as the catalyst. The ensuing suberin is highly esterified as observed by a precise Nuclear Magnetic Resonance quantification of each ester type, discriminating between primary and acylglycerol esters. Moreover, the composing hydrolysable monomers detected through GC-MS revealed that hexadecanoic acid is the most abundant monomer, similar to that reported before by others. Overall, this study highlights the adequacy of the ionic liquid catalyst for the isolation of suberin from soybean roots, where the polymer natural abundance is low, and builds new knowledge on the specificities of its chemistry; essential to better understand the biological roles of suberin in roots.
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spelling pubmed-104485292023-08-25 Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst Escórcio, Rita Sandhu, Armaan K. Bento, Artur Tomé, Ana S. Moreira, Carlos J. S. Brözel, Volker S. Silva Pereira, Cristina Front Chem Chemistry Soybean (Glycine max) is an increasingly relevant crop due to its economic importance and also a model plant for the study of root symbiotic associations with nodule forming rhizobia. Plant polyesters mediate plant-microbe interactions with both pathogenic and beneficial microbes; suberin has been hypothesized to play a key role during the early steps of rhizobia attachment to the root. The downside is that suberin chemistry in soybean root is still scarcely studied. This study addresses this outstanding question by reporting a straightforward workflow for a speedy purification of suberin from soybean root and for its subsequent detailed chemical analysis. To purify suberin, cholinium hexanoate (an ionic liquid) was used as the catalyst. The ensuing suberin is highly esterified as observed by a precise Nuclear Magnetic Resonance quantification of each ester type, discriminating between primary and acylglycerol esters. Moreover, the composing hydrolysable monomers detected through GC-MS revealed that hexadecanoic acid is the most abundant monomer, similar to that reported before by others. Overall, this study highlights the adequacy of the ionic liquid catalyst for the isolation of suberin from soybean roots, where the polymer natural abundance is low, and builds new knowledge on the specificities of its chemistry; essential to better understand the biological roles of suberin in roots. Frontiers Media S.A. 2023-08-10 /pmc/articles/PMC10448529/ /pubmed/37638099 http://dx.doi.org/10.3389/fchem.2023.1165234 Text en Copyright © 2023 Escórcio, Sandhu, Bento, Tomé, Moreira, Brözel and Silva Pereira. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Escórcio, Rita
Sandhu, Armaan K.
Bento, Artur
Tomé, Ana S.
Moreira, Carlos J. S.
Brözel, Volker S.
Silva Pereira, Cristina
Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_full Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_fullStr Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_full_unstemmed Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_short Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_sort purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448529/
https://www.ncbi.nlm.nih.gov/pubmed/37638099
http://dx.doi.org/10.3389/fchem.2023.1165234
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