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Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates

[Image: see text] Mesoporous silica microspheres (MPSMs) represent a promising material as a stationary phase for HPLC separations. The use of hard templates provides a preparation strategy for producing such monodisperse silica microspheres. Here, 15 MPSMs were systematically synthesized by varying...

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Autores principales: Steinbach, Julia C., Fait, Fabio, Mayer, Hermann A., Kandelbauer, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448668/
https://www.ncbi.nlm.nih.gov/pubmed/37636943
http://dx.doi.org/10.1021/acsomega.3c03098
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author Steinbach, Julia C.
Fait, Fabio
Mayer, Hermann A.
Kandelbauer, Andreas
author_facet Steinbach, Julia C.
Fait, Fabio
Mayer, Hermann A.
Kandelbauer, Andreas
author_sort Steinbach, Julia C.
collection PubMed
description [Image: see text] Mesoporous silica microspheres (MPSMs) represent a promising material as a stationary phase for HPLC separations. The use of hard templates provides a preparation strategy for producing such monodisperse silica microspheres. Here, 15 MPSMs were systematically synthesized by varying the sol–gel reaction parameters of water-to-precursor ratio and ammonia concentration in the presence of a porous p(GMA-co-EDMA) polymeric hard template. Changing the sol–gel process factors resulted in a wide range of MPSMs with varying particle sizes from smaller than one to several micrometers. The application of response surface methodology allowed to derive quantitative predictive models based on the process factor effects on particle size, pore size, pore volume, and specific surface area of the MPSMs. A narrow size distribution of the silica particles was maintained over the entire experimental space. Two larger-scale batches of MPSMs were prepared, and the particles were functionalized with trimethoxy(octadecyl) silane for the application as stationary phase in reversed-phases liquid chromatography. The separation of proteins and amino acids was successfully accomplished, and the effect of the pore properties of the silica particles on separation was demonstrated.
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spelling pubmed-104486682023-08-25 Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates Steinbach, Julia C. Fait, Fabio Mayer, Hermann A. Kandelbauer, Andreas ACS Omega [Image: see text] Mesoporous silica microspheres (MPSMs) represent a promising material as a stationary phase for HPLC separations. The use of hard templates provides a preparation strategy for producing such monodisperse silica microspheres. Here, 15 MPSMs were systematically synthesized by varying the sol–gel reaction parameters of water-to-precursor ratio and ammonia concentration in the presence of a porous p(GMA-co-EDMA) polymeric hard template. Changing the sol–gel process factors resulted in a wide range of MPSMs with varying particle sizes from smaller than one to several micrometers. The application of response surface methodology allowed to derive quantitative predictive models based on the process factor effects on particle size, pore size, pore volume, and specific surface area of the MPSMs. A narrow size distribution of the silica particles was maintained over the entire experimental space. Two larger-scale batches of MPSMs were prepared, and the particles were functionalized with trimethoxy(octadecyl) silane for the application as stationary phase in reversed-phases liquid chromatography. The separation of proteins and amino acids was successfully accomplished, and the effect of the pore properties of the silica particles on separation was demonstrated. American Chemical Society 2023-08-09 /pmc/articles/PMC10448668/ /pubmed/37636943 http://dx.doi.org/10.1021/acsomega.3c03098 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Steinbach, Julia C.
Fait, Fabio
Mayer, Hermann A.
Kandelbauer, Andreas
Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates
title Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates
title_full Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates
title_fullStr Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates
title_full_unstemmed Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates
title_short Sol–Gel-Controlled Size and Morphology of Mesoporous Silica Microspheres Using Hard Templates
title_sort sol–gel-controlled size and morphology of mesoporous silica microspheres using hard templates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448668/
https://www.ncbi.nlm.nih.gov/pubmed/37636943
http://dx.doi.org/10.1021/acsomega.3c03098
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