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Efficacy of Wex-cide 128 disinfectant against multiple prion strains

Prion diseases are transmissible, fatal neurologic diseases that include Creutzfeldt-Jakob Disease (CJD) in humans, chronic wasting disease (CWD) in cervids, bovine spongiform encephalopathy (BSE) in cattle and scrapie in sheep. Prions are extremely difficult to inactivate and established methods to...

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Autores principales: Baune, Chase, Groveman, Bradley R., Hughson, Andrew G., Thomas, Tina, Twardoski, Barry, Priola, Suzette, Chesebro, Bruce, Race, Brent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449212/
https://www.ncbi.nlm.nih.gov/pubmed/37616303
http://dx.doi.org/10.1371/journal.pone.0290325
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author Baune, Chase
Groveman, Bradley R.
Hughson, Andrew G.
Thomas, Tina
Twardoski, Barry
Priola, Suzette
Chesebro, Bruce
Race, Brent
author_facet Baune, Chase
Groveman, Bradley R.
Hughson, Andrew G.
Thomas, Tina
Twardoski, Barry
Priola, Suzette
Chesebro, Bruce
Race, Brent
author_sort Baune, Chase
collection PubMed
description Prion diseases are transmissible, fatal neurologic diseases that include Creutzfeldt-Jakob Disease (CJD) in humans, chronic wasting disease (CWD) in cervids, bovine spongiform encephalopathy (BSE) in cattle and scrapie in sheep. Prions are extremely difficult to inactivate and established methods to reduce prion infectivity are often dangerous, caustic, expensive, or impractical. Identifying viable and safe methods for treating prion contaminated materials is important for hospitals, research facilities, biologists, hunters, and meat-processors. For three decades, some prion researchers have used a phenolic product called Environ LpH (eLpH) to inactivate prions. ELpH has been discontinued, but a similar product, Wex-cide 128, containing the similar phenolic chemicals as eLpH is now available. In the current study, we directly compared the anti-prion efficacy of eLpH and Wex-cide 128 against prions from four different species (hamster 263K, cervid CWD, mouse 22L and human CJD). Decontamination was performed on either prion infected brain homogenates or prion contaminated steel wires and mouse bioassay was used to quantify the remaining prion infectivity. Our data show that both eLpH and Wex-cide 128 removed 4.0–5.5 logs of prion infectivity from 22L, CWD and 263K prion homogenates, but only about 1.25–1.50 logs of prion infectivity from human sporadic CJD. Wex-cide 128 is a viable substitute for inactivation of most prions from most species, but the resistance of CJD to phenolic inactivation is a concern and emphasizes the fact that inactivation methods should be confirmed for each target prion strain.
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spelling pubmed-104492122023-08-25 Efficacy of Wex-cide 128 disinfectant against multiple prion strains Baune, Chase Groveman, Bradley R. Hughson, Andrew G. Thomas, Tina Twardoski, Barry Priola, Suzette Chesebro, Bruce Race, Brent PLoS One Research Article Prion diseases are transmissible, fatal neurologic diseases that include Creutzfeldt-Jakob Disease (CJD) in humans, chronic wasting disease (CWD) in cervids, bovine spongiform encephalopathy (BSE) in cattle and scrapie in sheep. Prions are extremely difficult to inactivate and established methods to reduce prion infectivity are often dangerous, caustic, expensive, or impractical. Identifying viable and safe methods for treating prion contaminated materials is important for hospitals, research facilities, biologists, hunters, and meat-processors. For three decades, some prion researchers have used a phenolic product called Environ LpH (eLpH) to inactivate prions. ELpH has been discontinued, but a similar product, Wex-cide 128, containing the similar phenolic chemicals as eLpH is now available. In the current study, we directly compared the anti-prion efficacy of eLpH and Wex-cide 128 against prions from four different species (hamster 263K, cervid CWD, mouse 22L and human CJD). Decontamination was performed on either prion infected brain homogenates or prion contaminated steel wires and mouse bioassay was used to quantify the remaining prion infectivity. Our data show that both eLpH and Wex-cide 128 removed 4.0–5.5 logs of prion infectivity from 22L, CWD and 263K prion homogenates, but only about 1.25–1.50 logs of prion infectivity from human sporadic CJD. Wex-cide 128 is a viable substitute for inactivation of most prions from most species, but the resistance of CJD to phenolic inactivation is a concern and emphasizes the fact that inactivation methods should be confirmed for each target prion strain. Public Library of Science 2023-08-24 /pmc/articles/PMC10449212/ /pubmed/37616303 http://dx.doi.org/10.1371/journal.pone.0290325 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Baune, Chase
Groveman, Bradley R.
Hughson, Andrew G.
Thomas, Tina
Twardoski, Barry
Priola, Suzette
Chesebro, Bruce
Race, Brent
Efficacy of Wex-cide 128 disinfectant against multiple prion strains
title Efficacy of Wex-cide 128 disinfectant against multiple prion strains
title_full Efficacy of Wex-cide 128 disinfectant against multiple prion strains
title_fullStr Efficacy of Wex-cide 128 disinfectant against multiple prion strains
title_full_unstemmed Efficacy of Wex-cide 128 disinfectant against multiple prion strains
title_short Efficacy of Wex-cide 128 disinfectant against multiple prion strains
title_sort efficacy of wex-cide 128 disinfectant against multiple prion strains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449212/
https://www.ncbi.nlm.nih.gov/pubmed/37616303
http://dx.doi.org/10.1371/journal.pone.0290325
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