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Efficacy of Wex-cide 128 disinfectant against multiple prion strains
Prion diseases are transmissible, fatal neurologic diseases that include Creutzfeldt-Jakob Disease (CJD) in humans, chronic wasting disease (CWD) in cervids, bovine spongiform encephalopathy (BSE) in cattle and scrapie in sheep. Prions are extremely difficult to inactivate and established methods to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449212/ https://www.ncbi.nlm.nih.gov/pubmed/37616303 http://dx.doi.org/10.1371/journal.pone.0290325 |
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author | Baune, Chase Groveman, Bradley R. Hughson, Andrew G. Thomas, Tina Twardoski, Barry Priola, Suzette Chesebro, Bruce Race, Brent |
author_facet | Baune, Chase Groveman, Bradley R. Hughson, Andrew G. Thomas, Tina Twardoski, Barry Priola, Suzette Chesebro, Bruce Race, Brent |
author_sort | Baune, Chase |
collection | PubMed |
description | Prion diseases are transmissible, fatal neurologic diseases that include Creutzfeldt-Jakob Disease (CJD) in humans, chronic wasting disease (CWD) in cervids, bovine spongiform encephalopathy (BSE) in cattle and scrapie in sheep. Prions are extremely difficult to inactivate and established methods to reduce prion infectivity are often dangerous, caustic, expensive, or impractical. Identifying viable and safe methods for treating prion contaminated materials is important for hospitals, research facilities, biologists, hunters, and meat-processors. For three decades, some prion researchers have used a phenolic product called Environ LpH (eLpH) to inactivate prions. ELpH has been discontinued, but a similar product, Wex-cide 128, containing the similar phenolic chemicals as eLpH is now available. In the current study, we directly compared the anti-prion efficacy of eLpH and Wex-cide 128 against prions from four different species (hamster 263K, cervid CWD, mouse 22L and human CJD). Decontamination was performed on either prion infected brain homogenates or prion contaminated steel wires and mouse bioassay was used to quantify the remaining prion infectivity. Our data show that both eLpH and Wex-cide 128 removed 4.0–5.5 logs of prion infectivity from 22L, CWD and 263K prion homogenates, but only about 1.25–1.50 logs of prion infectivity from human sporadic CJD. Wex-cide 128 is a viable substitute for inactivation of most prions from most species, but the resistance of CJD to phenolic inactivation is a concern and emphasizes the fact that inactivation methods should be confirmed for each target prion strain. |
format | Online Article Text |
id | pubmed-10449212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104492122023-08-25 Efficacy of Wex-cide 128 disinfectant against multiple prion strains Baune, Chase Groveman, Bradley R. Hughson, Andrew G. Thomas, Tina Twardoski, Barry Priola, Suzette Chesebro, Bruce Race, Brent PLoS One Research Article Prion diseases are transmissible, fatal neurologic diseases that include Creutzfeldt-Jakob Disease (CJD) in humans, chronic wasting disease (CWD) in cervids, bovine spongiform encephalopathy (BSE) in cattle and scrapie in sheep. Prions are extremely difficult to inactivate and established methods to reduce prion infectivity are often dangerous, caustic, expensive, or impractical. Identifying viable and safe methods for treating prion contaminated materials is important for hospitals, research facilities, biologists, hunters, and meat-processors. For three decades, some prion researchers have used a phenolic product called Environ LpH (eLpH) to inactivate prions. ELpH has been discontinued, but a similar product, Wex-cide 128, containing the similar phenolic chemicals as eLpH is now available. In the current study, we directly compared the anti-prion efficacy of eLpH and Wex-cide 128 against prions from four different species (hamster 263K, cervid CWD, mouse 22L and human CJD). Decontamination was performed on either prion infected brain homogenates or prion contaminated steel wires and mouse bioassay was used to quantify the remaining prion infectivity. Our data show that both eLpH and Wex-cide 128 removed 4.0–5.5 logs of prion infectivity from 22L, CWD and 263K prion homogenates, but only about 1.25–1.50 logs of prion infectivity from human sporadic CJD. Wex-cide 128 is a viable substitute for inactivation of most prions from most species, but the resistance of CJD to phenolic inactivation is a concern and emphasizes the fact that inactivation methods should be confirmed for each target prion strain. Public Library of Science 2023-08-24 /pmc/articles/PMC10449212/ /pubmed/37616303 http://dx.doi.org/10.1371/journal.pone.0290325 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Baune, Chase Groveman, Bradley R. Hughson, Andrew G. Thomas, Tina Twardoski, Barry Priola, Suzette Chesebro, Bruce Race, Brent Efficacy of Wex-cide 128 disinfectant against multiple prion strains |
title | Efficacy of Wex-cide 128 disinfectant against multiple prion strains |
title_full | Efficacy of Wex-cide 128 disinfectant against multiple prion strains |
title_fullStr | Efficacy of Wex-cide 128 disinfectant against multiple prion strains |
title_full_unstemmed | Efficacy of Wex-cide 128 disinfectant against multiple prion strains |
title_short | Efficacy of Wex-cide 128 disinfectant against multiple prion strains |
title_sort | efficacy of wex-cide 128 disinfectant against multiple prion strains |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449212/ https://www.ncbi.nlm.nih.gov/pubmed/37616303 http://dx.doi.org/10.1371/journal.pone.0290325 |
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