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Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals

Desmodus rotundus, vampire bats, transmit dangerous infections, and brucellosis is a hazardous zoonotic disease, two adversities that coexist in the subtropical and tropical areas of the American continent. Here, we report a 47.89% Brucella infection prevalence in a colony of vampire bats inhabiting...

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Autores principales: Hernández-Mora, Gabriela, Chacón-Díaz, Carlos, Moreira-Soto, Andres, Barrantes-Granados, Osvaldo, Suárez-Esquivel, Marcela, Viquez-Ruiz, Eunice, Barquero-Calvo, Elías, Ruiz-Villalobos, Nazareth, Hidalgo-Montealegre, Daniela, González-Barrientos, Rocío, Demeter, Elena A., Estrella-Morales, Josimar, Zúñiga-Pereira, Ana-Mariel, Quesada-Gómez, Carlos, Chaves-Olarte, Esteban, Lomonte, Bruno, Guzmán-Verri, Caterina, Drexler, Jan Felix, Moreno, Edgardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449500/
https://www.ncbi.nlm.nih.gov/pubmed/37404031
http://dx.doi.org/10.1128/msphere.00061-23
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author Hernández-Mora, Gabriela
Chacón-Díaz, Carlos
Moreira-Soto, Andres
Barrantes-Granados, Osvaldo
Suárez-Esquivel, Marcela
Viquez-Ruiz, Eunice
Barquero-Calvo, Elías
Ruiz-Villalobos, Nazareth
Hidalgo-Montealegre, Daniela
González-Barrientos, Rocío
Demeter, Elena A.
Estrella-Morales, Josimar
Zúñiga-Pereira, Ana-Mariel
Quesada-Gómez, Carlos
Chaves-Olarte, Esteban
Lomonte, Bruno
Guzmán-Verri, Caterina
Drexler, Jan Felix
Moreno, Edgardo
author_facet Hernández-Mora, Gabriela
Chacón-Díaz, Carlos
Moreira-Soto, Andres
Barrantes-Granados, Osvaldo
Suárez-Esquivel, Marcela
Viquez-Ruiz, Eunice
Barquero-Calvo, Elías
Ruiz-Villalobos, Nazareth
Hidalgo-Montealegre, Daniela
González-Barrientos, Rocío
Demeter, Elena A.
Estrella-Morales, Josimar
Zúñiga-Pereira, Ana-Mariel
Quesada-Gómez, Carlos
Chaves-Olarte, Esteban
Lomonte, Bruno
Guzmán-Verri, Caterina
Drexler, Jan Felix
Moreno, Edgardo
author_sort Hernández-Mora, Gabriela
collection PubMed
description Desmodus rotundus, vampire bats, transmit dangerous infections, and brucellosis is a hazardous zoonotic disease, two adversities that coexist in the subtropical and tropical areas of the American continent. Here, we report a 47.89% Brucella infection prevalence in a colony of vampire bats inhabiting the tropical rainforest of Costa Rica. The bacterium induced placentitis and fetal death in bats. Wide-range phenotypic and genotypic characterization placed the Brucella organisms as a new pathogenic species named Brucella nosferati sp. nov., isolated from bat tissues, including the salivary glands, suggesting feeding behavior might favor transmission to their prey. Overall analyses placed B. nosferati as the etiological agent of a reported canine brucellosis case, demonstrating its potential for infecting other hosts. To assess the putative prey hosts, we analyzed the intestinal contents of 14 infected and 23 non-infected bats by proteomics. A total of 54,508 peptides sorted into 7,203 unique peptides corresponding to 1,521 proteins were identified. Twenty-three wildlife and domestic taxa, including humans, were foraged by B. nosferati-infected D. rotundus, suggesting contact of this bacterium with a broad range of hosts. Our approach is appropriate for detecting, in a single study, the prey preferences of vampire bats in a diverse area, demonstrating its suitability for control strategies where vampire bats thrive. IMPORTANCE: The discovery that a high proportion of vampire bats in a tropical area is infected with pathogenic Brucella nosferati and that bats forage on humans and many wild and domestic animals is relevant from the perspective of emerging disease prevention. Indeed, bats harboring B. nosferati in their salivary glands may transmit this pathogenic bacterium to other hosts. This potential is not trivial since, besides the demonstrated pathogenicity, this bacterium possesses all the required virulent arsenal of dangerous Brucella organisms, including those that are zoonotic for humans. Our work has settled the basis for future surveillance actions in brucellosis control programs where these infected bats thrive. Moreover, our strategy to identify the foraging range of bats may be adapted for exploring the feeding habits of diverse animals, including arthropod vectors of infectious diseases, and therefore of interest to a broader audience besides experts on Brucella and bats.
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spelling pubmed-104495002023-08-25 Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals Hernández-Mora, Gabriela Chacón-Díaz, Carlos Moreira-Soto, Andres Barrantes-Granados, Osvaldo Suárez-Esquivel, Marcela Viquez-Ruiz, Eunice Barquero-Calvo, Elías Ruiz-Villalobos, Nazareth Hidalgo-Montealegre, Daniela González-Barrientos, Rocío Demeter, Elena A. Estrella-Morales, Josimar Zúñiga-Pereira, Ana-Mariel Quesada-Gómez, Carlos Chaves-Olarte, Esteban Lomonte, Bruno Guzmán-Verri, Caterina Drexler, Jan Felix Moreno, Edgardo mSphere Research Article Desmodus rotundus, vampire bats, transmit dangerous infections, and brucellosis is a hazardous zoonotic disease, two adversities that coexist in the subtropical and tropical areas of the American continent. Here, we report a 47.89% Brucella infection prevalence in a colony of vampire bats inhabiting the tropical rainforest of Costa Rica. The bacterium induced placentitis and fetal death in bats. Wide-range phenotypic and genotypic characterization placed the Brucella organisms as a new pathogenic species named Brucella nosferati sp. nov., isolated from bat tissues, including the salivary glands, suggesting feeding behavior might favor transmission to their prey. Overall analyses placed B. nosferati as the etiological agent of a reported canine brucellosis case, demonstrating its potential for infecting other hosts. To assess the putative prey hosts, we analyzed the intestinal contents of 14 infected and 23 non-infected bats by proteomics. A total of 54,508 peptides sorted into 7,203 unique peptides corresponding to 1,521 proteins were identified. Twenty-three wildlife and domestic taxa, including humans, were foraged by B. nosferati-infected D. rotundus, suggesting contact of this bacterium with a broad range of hosts. Our approach is appropriate for detecting, in a single study, the prey preferences of vampire bats in a diverse area, demonstrating its suitability for control strategies where vampire bats thrive. IMPORTANCE: The discovery that a high proportion of vampire bats in a tropical area is infected with pathogenic Brucella nosferati and that bats forage on humans and many wild and domestic animals is relevant from the perspective of emerging disease prevention. Indeed, bats harboring B. nosferati in their salivary glands may transmit this pathogenic bacterium to other hosts. This potential is not trivial since, besides the demonstrated pathogenicity, this bacterium possesses all the required virulent arsenal of dangerous Brucella organisms, including those that are zoonotic for humans. Our work has settled the basis for future surveillance actions in brucellosis control programs where these infected bats thrive. Moreover, our strategy to identify the foraging range of bats may be adapted for exploring the feeding habits of diverse animals, including arthropod vectors of infectious diseases, and therefore of interest to a broader audience besides experts on Brucella and bats. American Society for Microbiology 2023-07-05 /pmc/articles/PMC10449500/ /pubmed/37404031 http://dx.doi.org/10.1128/msphere.00061-23 Text en Copyright © 2023 Hernández-Mora et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Hernández-Mora, Gabriela
Chacón-Díaz, Carlos
Moreira-Soto, Andres
Barrantes-Granados, Osvaldo
Suárez-Esquivel, Marcela
Viquez-Ruiz, Eunice
Barquero-Calvo, Elías
Ruiz-Villalobos, Nazareth
Hidalgo-Montealegre, Daniela
González-Barrientos, Rocío
Demeter, Elena A.
Estrella-Morales, Josimar
Zúñiga-Pereira, Ana-Mariel
Quesada-Gómez, Carlos
Chaves-Olarte, Esteban
Lomonte, Bruno
Guzmán-Verri, Caterina
Drexler, Jan Felix
Moreno, Edgardo
Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
title Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
title_full Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
title_fullStr Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
title_full_unstemmed Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
title_short Virulent Brucella nosferati infecting Desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
title_sort virulent brucella nosferati infecting desmodus rotundus has emerging potential due to the broad foraging range of its bat host for humans and wild and domestic animals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449500/
https://www.ncbi.nlm.nih.gov/pubmed/37404031
http://dx.doi.org/10.1128/msphere.00061-23
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