Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations

We determined cytokine levels in paired serum/CSF samples from first-episode schizophrenia (FES) participants (n = 20) and controls (n = 21) using a 13-plex immunoassay. Applying strictly-determined detection limits, 12 cytokines were found in serum and two in CSF. Higher serum MCP-1 levels (p = 0.0...

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Autores principales: Singh, Deepti, Guest, Paul C., Dobrowolny, Henrik, Fischbach, Tino, Meyer-Lotz, Gabriela, Breitling-Ziegler, Carolin, Haghikia, Aiden, Vielhaber, Stefan, Steiner, Johann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449694/
https://www.ncbi.nlm.nih.gov/pubmed/36773080
http://dx.doi.org/10.1007/s00406-023-01569-y
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author Singh, Deepti
Guest, Paul C.
Dobrowolny, Henrik
Fischbach, Tino
Meyer-Lotz, Gabriela
Breitling-Ziegler, Carolin
Haghikia, Aiden
Vielhaber, Stefan
Steiner, Johann
author_facet Singh, Deepti
Guest, Paul C.
Dobrowolny, Henrik
Fischbach, Tino
Meyer-Lotz, Gabriela
Breitling-Ziegler, Carolin
Haghikia, Aiden
Vielhaber, Stefan
Steiner, Johann
author_sort Singh, Deepti
collection PubMed
description We determined cytokine levels in paired serum/CSF samples from first-episode schizophrenia (FES) participants (n = 20) and controls (n = 21) using a 13-plex immunoassay. Applying strictly-determined detection limits, 12 cytokines were found in serum and two in CSF. Higher serum MCP-1 levels (p = 0.007) were present in FES versus controls, which correlated with serum IgG (R = − 0.750; p = 0.013). Finally, IL-18 levels correlated with body weight in FES (R = 0.691; p = 0.041). This study demonstrates potential limitations in the sensitivity of multiplex cytokine assays for CSF studies in mental disorders and suggests that some published studies in this area should be re-evaluated. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00406-023-01569-y.
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spelling pubmed-104496942023-08-26 Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations Singh, Deepti Guest, Paul C. Dobrowolny, Henrik Fischbach, Tino Meyer-Lotz, Gabriela Breitling-Ziegler, Carolin Haghikia, Aiden Vielhaber, Stefan Steiner, Johann Eur Arch Psychiatry Clin Neurosci Short Communication We determined cytokine levels in paired serum/CSF samples from first-episode schizophrenia (FES) participants (n = 20) and controls (n = 21) using a 13-plex immunoassay. Applying strictly-determined detection limits, 12 cytokines were found in serum and two in CSF. Higher serum MCP-1 levels (p = 0.007) were present in FES versus controls, which correlated with serum IgG (R = − 0.750; p = 0.013). Finally, IL-18 levels correlated with body weight in FES (R = 0.691; p = 0.041). This study demonstrates potential limitations in the sensitivity of multiplex cytokine assays for CSF studies in mental disorders and suggests that some published studies in this area should be re-evaluated. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00406-023-01569-y. Springer Berlin Heidelberg 2023-02-11 2023 /pmc/articles/PMC10449694/ /pubmed/36773080 http://dx.doi.org/10.1007/s00406-023-01569-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Short Communication
Singh, Deepti
Guest, Paul C.
Dobrowolny, Henrik
Fischbach, Tino
Meyer-Lotz, Gabriela
Breitling-Ziegler, Carolin
Haghikia, Aiden
Vielhaber, Stefan
Steiner, Johann
Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
title Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
title_full Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
title_fullStr Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
title_full_unstemmed Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
title_short Cytokine alterations in CSF and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
title_sort cytokine alterations in csf and serum samples of patients with a first episode of schizophrenia: results and methodological considerations
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449694/
https://www.ncbi.nlm.nih.gov/pubmed/36773080
http://dx.doi.org/10.1007/s00406-023-01569-y
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