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Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats

As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon d...

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Autores principales: Zhang, Meiling, Cheng, Jinjun, Luo, Juan, Li, Changxiang, Hou, Tingting, Zhao, Yan, Wang, Yaoxian, Qu, Huihua, Kong, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450154/
https://www.ncbi.nlm.nih.gov/pubmed/37637430
http://dx.doi.org/10.3389/fphar.2023.1118550
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author Zhang, Meiling
Cheng, Jinjun
Luo, Juan
Li, Changxiang
Hou, Tingting
Zhao, Yan
Wang, Yaoxian
Qu, Huihua
Kong, Hui
author_facet Zhang, Meiling
Cheng, Jinjun
Luo, Juan
Li, Changxiang
Hou, Tingting
Zhao, Yan
Wang, Yaoxian
Qu, Huihua
Kong, Hui
author_sort Zhang, Meiling
collection PubMed
description As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon dots (SRC-CDs) were extracted and purified from the aqueous solution of SRC, followed by physicochemical property assessment by series of technologies. The cooling blood and hemostatic effects of SRC-CDs were further evaluated via a blood-heat and hemorrhage (BHH) rat model. Results showed that the diameters of obtained fluorescent SRC-CDs ranged from 5.0 nm to 10.0 nm and possessed functional group-rich surfaces. Additionally, the as-prepared SRC-CDs showed remarkable cooling blood and hemostasis effects in BHH model, mainly manifested by significant improvement of elevated rectal temperature, inflammatory cytokines (TNF-α, IL-6, and IL-1β) levels, as well as protein expressions of myD88 and NF-κB p65, abnormal coagulation parameters (elevated APTT and FIB), hemogram parameters (RBC, HGB, and HCT), and histopathological changes in lung and gastric tissues. This study, for the first time, demonstrated that SRC-CDs were the cooling blood and hemostatic active components of SRC, which could inhibit the release of inflammatory cytokines by regulating myD88/NF-κB signaling pathway, and activating the fibrin system and endogenous coagulation pathway. These results not only provide a new perspective for the study of active ingredients of carbonized herbs represented by SRC, but also lay an experimental foundation for the development of next-generation nanomedicines.
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spelling pubmed-104501542023-08-26 Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats Zhang, Meiling Cheng, Jinjun Luo, Juan Li, Changxiang Hou, Tingting Zhao, Yan Wang, Yaoxian Qu, Huihua Kong, Hui Front Pharmacol Pharmacology As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon dots (SRC-CDs) were extracted and purified from the aqueous solution of SRC, followed by physicochemical property assessment by series of technologies. The cooling blood and hemostatic effects of SRC-CDs were further evaluated via a blood-heat and hemorrhage (BHH) rat model. Results showed that the diameters of obtained fluorescent SRC-CDs ranged from 5.0 nm to 10.0 nm and possessed functional group-rich surfaces. Additionally, the as-prepared SRC-CDs showed remarkable cooling blood and hemostasis effects in BHH model, mainly manifested by significant improvement of elevated rectal temperature, inflammatory cytokines (TNF-α, IL-6, and IL-1β) levels, as well as protein expressions of myD88 and NF-κB p65, abnormal coagulation parameters (elevated APTT and FIB), hemogram parameters (RBC, HGB, and HCT), and histopathological changes in lung and gastric tissues. This study, for the first time, demonstrated that SRC-CDs were the cooling blood and hemostatic active components of SRC, which could inhibit the release of inflammatory cytokines by regulating myD88/NF-κB signaling pathway, and activating the fibrin system and endogenous coagulation pathway. These results not only provide a new perspective for the study of active ingredients of carbonized herbs represented by SRC, but also lay an experimental foundation for the development of next-generation nanomedicines. Frontiers Media S.A. 2023-08-10 /pmc/articles/PMC10450154/ /pubmed/37637430 http://dx.doi.org/10.3389/fphar.2023.1118550 Text en Copyright © 2023 Zhang, Cheng, Luo, Li, Hou, Zhao, Wang, Qu and Kong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhang, Meiling
Cheng, Jinjun
Luo, Juan
Li, Changxiang
Hou, Tingting
Zhao, Yan
Wang, Yaoxian
Qu, Huihua
Kong, Hui
Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
title Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
title_full Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
title_fullStr Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
title_full_unstemmed Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
title_short Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
title_sort protective effects of scutellariae radix carbonisata-derived carbon dots on blood-heat and hemorrhage rats
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450154/
https://www.ncbi.nlm.nih.gov/pubmed/37637430
http://dx.doi.org/10.3389/fphar.2023.1118550
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