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Matrix scaffolds for endometrium-derived organoid models

The uterus-lining endometrium is essential to mammalian reproduction, receiving and accommodating the embryo for proper development. Despite its key role, mechanisms underlying endometrial biology (menstrual cycling, embryo interaction) and disease are not well understood. Its hidden location in the...

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Autores principales: De Vriendt, Silke, Casares, Celia Mesias, Rocha, Susana, Vankelecom, Hugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450215/
https://www.ncbi.nlm.nih.gov/pubmed/37635971
http://dx.doi.org/10.3389/fendo.2023.1240064
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author De Vriendt, Silke
Casares, Celia Mesias
Rocha, Susana
Vankelecom, Hugo
author_facet De Vriendt, Silke
Casares, Celia Mesias
Rocha, Susana
Vankelecom, Hugo
author_sort De Vriendt, Silke
collection PubMed
description The uterus-lining endometrium is essential to mammalian reproduction, receiving and accommodating the embryo for proper development. Despite its key role, mechanisms underlying endometrial biology (menstrual cycling, embryo interaction) and disease are not well understood. Its hidden location in the womb, and thereby-associated lack of suitable research models, contribute to this knowledge gap. Recently, 3D organoid models have been developed from both healthy and diseased endometrium. These organoids closely recapitulate the tissue’s epithelium phenotype and (patho)biology, including in vitro reproduction of the menstrual cycle. Typically, organoids are grown in a scaffold made of surrogate tissue extracellular matrix (ECM), with mouse tumor basement membrane extracts being the most commonly used. However, important limitations apply including their lack of standardization and xeno-derivation which strongly hinder clinical translation. Therefore, researchers are actively seeking better alternatives including fully defined matrices for faithful and efficient growth of organoids. Here, we summarize the state-of-the-art regarding matrix scaffolds to grow endometrium-derived organoids as well as more advanced organoid-based 3D models. We discuss remaining shortcomings and challenges to advance endometrial organoids toward defined and standardized tools for applications in basic research and translational/clinical fields.
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spelling pubmed-104502152023-08-26 Matrix scaffolds for endometrium-derived organoid models De Vriendt, Silke Casares, Celia Mesias Rocha, Susana Vankelecom, Hugo Front Endocrinol (Lausanne) Endocrinology The uterus-lining endometrium is essential to mammalian reproduction, receiving and accommodating the embryo for proper development. Despite its key role, mechanisms underlying endometrial biology (menstrual cycling, embryo interaction) and disease are not well understood. Its hidden location in the womb, and thereby-associated lack of suitable research models, contribute to this knowledge gap. Recently, 3D organoid models have been developed from both healthy and diseased endometrium. These organoids closely recapitulate the tissue’s epithelium phenotype and (patho)biology, including in vitro reproduction of the menstrual cycle. Typically, organoids are grown in a scaffold made of surrogate tissue extracellular matrix (ECM), with mouse tumor basement membrane extracts being the most commonly used. However, important limitations apply including their lack of standardization and xeno-derivation which strongly hinder clinical translation. Therefore, researchers are actively seeking better alternatives including fully defined matrices for faithful and efficient growth of organoids. Here, we summarize the state-of-the-art regarding matrix scaffolds to grow endometrium-derived organoids as well as more advanced organoid-based 3D models. We discuss remaining shortcomings and challenges to advance endometrial organoids toward defined and standardized tools for applications in basic research and translational/clinical fields. Frontiers Media S.A. 2023-08-10 /pmc/articles/PMC10450215/ /pubmed/37635971 http://dx.doi.org/10.3389/fendo.2023.1240064 Text en Copyright © 2023 De Vriendt, Casares, Rocha and Vankelecom https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
De Vriendt, Silke
Casares, Celia Mesias
Rocha, Susana
Vankelecom, Hugo
Matrix scaffolds for endometrium-derived organoid models
title Matrix scaffolds for endometrium-derived organoid models
title_full Matrix scaffolds for endometrium-derived organoid models
title_fullStr Matrix scaffolds for endometrium-derived organoid models
title_full_unstemmed Matrix scaffolds for endometrium-derived organoid models
title_short Matrix scaffolds for endometrium-derived organoid models
title_sort matrix scaffolds for endometrium-derived organoid models
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450215/
https://www.ncbi.nlm.nih.gov/pubmed/37635971
http://dx.doi.org/10.3389/fendo.2023.1240064
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