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Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development

BACKGROUND AND AIM: The temporomandibular joint (TMJ) is a synovial joint that allows the complex movements essential for life. It connects the jawbone to the skull, working as a sliding hinge. Moreover, pluripotent stem cells are a source of precursors and tissue-specific cells in developing organi...

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Autores principales: Pagni, Tacia Catharine, Cunha, Juliana Malta da, Saez, Daniel Martinez, Costa-Neves, Adriana da, Kerkis, Irina, Silva, Marcelo Cavenaghi Pereira da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450518/
https://www.ncbi.nlm.nih.gov/pubmed/37637855
http://dx.doi.org/10.1016/j.jobcr.2023.08.002
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author Pagni, Tacia Catharine
Cunha, Juliana Malta da
Saez, Daniel Martinez
Costa-Neves, Adriana da
Kerkis, Irina
Silva, Marcelo Cavenaghi Pereira da
author_facet Pagni, Tacia Catharine
Cunha, Juliana Malta da
Saez, Daniel Martinez
Costa-Neves, Adriana da
Kerkis, Irina
Silva, Marcelo Cavenaghi Pereira da
author_sort Pagni, Tacia Catharine
collection PubMed
description BACKGROUND AND AIM: The temporomandibular joint (TMJ) is a synovial joint that allows the complex movements essential for life. It connects the jawbone to the skull, working as a sliding hinge. Moreover, pluripotent stem cells are a source of precursors and tissue-specific cells in developing organisms, however, their biodistribution in developing fetal tissues is weakly studied. The aim of our study was analyse immunohistochemical expression of Nanog, Oct-4, Sox-2 and Stat-3 and Sox-5, in TMJ tissue samples from human fetuses aged between the 12th and 20th weeks of intrauterine life. MATERIALS AND METHODS: We fixed and processed TMJ tissue samples from human fetuses, histological sections and immunohistochemical procedures were carried out. RESULTS: TMJ histological studies examination did not reveal any difference in the tissue organization between the samples in the studied periods. Immunohistochemical analysis demonstrated that Oct-4 and Sox-2 lack their expression in TMJ. In contrast, Nanog was expressed in nucleous of proliferative layer of mandibular condyle, Stat-3 was expressed in nuclear cells of articular disc, Stat-3 and Sox-5 showed positive nuclear and cytoplasmic immunostaining in codrocyte layers and in ossification areas. CONCLUSIONS: Nanog acts in maintanence of pluripotency, Stat-3 in articular disc acts as a transcriptional factor. Stat-3 and Sox-2 act in chondrocyte and osteoblast diferentiation. Distribution of the cells, which express Nanog, Stat-3, and Sox-5 in TMJ tissue during fetal development, can help further understand its physiology, pathology, and repairing capacities.
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spelling pubmed-104505182023-08-26 Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development Pagni, Tacia Catharine Cunha, Juliana Malta da Saez, Daniel Martinez Costa-Neves, Adriana da Kerkis, Irina Silva, Marcelo Cavenaghi Pereira da J Oral Biol Craniofac Res Article BACKGROUND AND AIM: The temporomandibular joint (TMJ) is a synovial joint that allows the complex movements essential for life. It connects the jawbone to the skull, working as a sliding hinge. Moreover, pluripotent stem cells are a source of precursors and tissue-specific cells in developing organisms, however, their biodistribution in developing fetal tissues is weakly studied. The aim of our study was analyse immunohistochemical expression of Nanog, Oct-4, Sox-2 and Stat-3 and Sox-5, in TMJ tissue samples from human fetuses aged between the 12th and 20th weeks of intrauterine life. MATERIALS AND METHODS: We fixed and processed TMJ tissue samples from human fetuses, histological sections and immunohistochemical procedures were carried out. RESULTS: TMJ histological studies examination did not reveal any difference in the tissue organization between the samples in the studied periods. Immunohistochemical analysis demonstrated that Oct-4 and Sox-2 lack their expression in TMJ. In contrast, Nanog was expressed in nucleous of proliferative layer of mandibular condyle, Stat-3 was expressed in nuclear cells of articular disc, Stat-3 and Sox-5 showed positive nuclear and cytoplasmic immunostaining in codrocyte layers and in ossification areas. CONCLUSIONS: Nanog acts in maintanence of pluripotency, Stat-3 in articular disc acts as a transcriptional factor. Stat-3 and Sox-2 act in chondrocyte and osteoblast diferentiation. Distribution of the cells, which express Nanog, Stat-3, and Sox-5 in TMJ tissue during fetal development, can help further understand its physiology, pathology, and repairing capacities. Elsevier 2023 2023-08-14 /pmc/articles/PMC10450518/ /pubmed/37637855 http://dx.doi.org/10.1016/j.jobcr.2023.08.002 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Pagni, Tacia Catharine
Cunha, Juliana Malta da
Saez, Daniel Martinez
Costa-Neves, Adriana da
Kerkis, Irina
Silva, Marcelo Cavenaghi Pereira da
Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development
title Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development
title_full Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development
title_fullStr Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development
title_full_unstemmed Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development
title_short Nanog, Stat-3, and Sox-5 involvement in human fetal temporomandibular joint late development
title_sort nanog, stat-3, and sox-5 involvement in human fetal temporomandibular joint late development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450518/
https://www.ncbi.nlm.nih.gov/pubmed/37637855
http://dx.doi.org/10.1016/j.jobcr.2023.08.002
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