Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography

Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HP...

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Detalles Bibliográficos
Autores principales: Kaewbai-Ngam, Janine, Incharoensakdi, Aran, Monshupanee, Tanakarn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450728/
https://www.ncbi.nlm.nih.gov/pubmed/37638303
http://dx.doi.org/10.21769/BioProtoc.4790
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author Kaewbai-Ngam, Janine
Incharoensakdi, Aran
Monshupanee, Tanakarn
author_facet Kaewbai-Ngam, Janine
Incharoensakdi, Aran
Monshupanee, Tanakarn
author_sort Kaewbai-Ngam, Janine
collection PubMed
description Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HPLC analysis is rapid, with a running time of approximately 5 min per sample. The technique can accurately determine PHB concentrations in the range of 2–1,000 μg/mL PHB. However, this technique is not applicable for determining the contents of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in cyanobacteria.
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spelling pubmed-104507282023-08-26 Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography Kaewbai-Ngam, Janine Incharoensakdi, Aran Monshupanee, Tanakarn Bio Protoc Methods Article Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HPLC analysis is rapid, with a running time of approximately 5 min per sample. The technique can accurately determine PHB concentrations in the range of 2–1,000 μg/mL PHB. However, this technique is not applicable for determining the contents of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in cyanobacteria. Bio-Protocol 2023-08-20 /pmc/articles/PMC10450728/ /pubmed/37638303 http://dx.doi.org/10.21769/BioProtoc.4790 Text en ©Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Methods Article
Kaewbai-Ngam, Janine
Incharoensakdi, Aran
Monshupanee, Tanakarn
Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
title Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
title_full Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
title_fullStr Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
title_full_unstemmed Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
title_short Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
title_sort determination of poly(3-hydroxybutyrate) content in cyanobacterium synechocystis sp. pcc 6803 using acid hydrolysis followed by high-performance liquid chromatography
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450728/
https://www.ncbi.nlm.nih.gov/pubmed/37638303
http://dx.doi.org/10.21769/BioProtoc.4790
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